CDCA8 (NM_018101) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CDCA8 |
| Synonyms | BOR; BOREALIN; DasraB; MESRGP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_018101 |
| ORF Size | 840 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018101.4 |
| RefSeq Size | 2405 bp |
| RefSeq ORF | 843 bp |
| Locus ID | 55143 |
| UniProt ID | Q53HL2 |
| Cytogenetics | 1p34.3 |
| Protein Families | Druggable Genome |
| MW | 31.3 kDa |
| Summary | This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. provided by RefSeq, Apr 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018101" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201385L1 | Lenti ORF clone of Human cell division cycle associated 8 (CDCA8), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201385L2 | Lenti ORF clone of Human cell division cycle associated 8 (CDCA8), mGFP tagged | 10 ug |
$600.00
|
|
| RC201385L3 | Lenti ORF clone of Human cell division cycle associated 8 (CDCA8), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201385L4 | Lenti ORF clone of Human cell division cycle associated 8 (CDCA8), mGFP tagged | 10 ug |
$600.00
|
|
| RG201385 | CDCA8 (tGFP-tagged) - Human cell division cycle associated 8 (CDCA8) | 10 ug |
$489.00
$500.00
|
|
| SC113781 | CDCA8 (untagged)-Human cell division cycle associated 8 (CDCA8) | 10 ug |
$300.00
|
|
Citations
Silencing CDCA8 Suppresses Hepatocellular Carcinoma Growth and Stemness via Restoration of ATF3 Tumor Suppressor and Inactivation of AKT/β-Catenin Signaling
Jeon, Ko, Seo et al
Cancers (Basel) (2021) 13 (5) DOI: 10.3390/cancers13051055
Jeon, Ko, Seo et al
Cancers (Basel) (2021) 13 (5) DOI: 10.3390/cancers13051055
Cited by 64
AI Summary: In this study, OriGene products including CDCA8 shRNA (SR319051), CDCA8 cDNA clone (RC210184), and ATF3 antibody (TA500494) were used to manipulate and detect gene expression, enabling the authors to investigate the role of CDCA8 in hepatocellular carcinoma growth and stemness.
Silencing CDCA8 Suppresses Hepatocellular Carcinoma Growth and Stemness via Restoration of ATF3 Tumor Suppressor and Inactivation of AKT/β–Catenin Signaling
Jeon, Ko, Seo et al
Cancers (2021) 13 (5), 1055 DOI: 10.3390/cancers13051055
Jeon, Ko, Seo et al
Cancers (2021) 13 (5), 1055 DOI: 10.3390/cancers13051055
Cited by 64
AI Summary: OriGene’s CDCA8-expressing vector (CDCA8-vector, RC201385) was used in this study to overexpress CDCA8 in hepatocellular carcinoma cells, enabling the authors to investigate the functional effects of CDCA8 on tumor growth and stemness.
Snippet: CDCA8-expressing vector (CDCA8-vector, RC201385) (all from OriGene, Rockville, MD, USA) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol
Showing 1-2 of 2 papers.
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