CoCoA (CALCOCO1) (NM_020898) Human Tagged ORF Clone
SKU
RC201375
CALCOCO1 (Myc-DDK-tagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1
$633.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CoCoA |
| Synonyms | calphoglin; Cocoa; PP13275 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020898 |
| ORF Size | 2073 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020898.3 |
| RefSeq Size | 3046 bp |
| RefSeq ORF | 2076 bp |
| Locus ID | 57658 |
| UniProt ID | Q9P1Z2 |
| Cytogenetics | 12q13.13 |
| Protein Families | Transcription Factors |
| MW | 77.3 kDa |
| Summary | Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity). In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020898" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201375L3 | Lenti ORF clone of Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$933.00
|
|
| RC201375L4 | Lenti ORF clone of Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1, mGFP tagged | 10 ug |
$933.00
|
|
| RG201375 | CALCOCO1 (tGFP-tagged) - Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1 | 10 ug |
$789.00
$833.00
|
|
| SC108014 | CALCOCO1 (untagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1 | 10 ug |
$634.00
|
|
Citations
Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy
Stefely, Zhang, Freiberger et al
Autophagy (2020)
Stefely, Zhang, Freiberger et al
Autophagy (2020)
Cited by 59
AI Summary: OriGene’s CALCOCO1-MYCDDK plasmid (RC201375) was used in this study to investigate the role of CALCOCO1 in MTOR-regulated selective autophagy, enabling the authors to analyze CALCOCO1 protein function and interactions.
Snippet: AbstractMacroautophagy/autophagy is suppressed by MTOR (mechanistic target of rapamycin kinase) and is an anticancer target under active investigation. Yet, MTOR-regulated autophagy remains incompl...;CALCOCO1-MYCDDK in pCMV6-Entry vector was purchased from OriGene (RC201375). …
Showing 1-1 of 1 papers.
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