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CD2BP2 (NM_006110) Human Tagged ORF Clone

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SKU
RC201352
CD2BP2 (Myc-DDK-tagged)-Human CD2 (cytoplasmic tail) binding protein 2 (CD2BP2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD2BP2
Synonyms FWP010; LIN1; PPP1R59; Snu40; U5-52K
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201352 representing NM_006110
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAAAGAGGAAAGTGACCTTCCAAGGCGTGGGAGATGAGGAGGATGAGGATGAAATCATTGTCCCCA
AGAAGAAGCTGGTGGACCCTGTGGCTGGGTCAGGGGGTCCTGGGAGCCGCTTTAAAGGCAAACACTCTTT
GGATAGCGATGAGGAGGAGGATGATGATGATGGGGGGTCCAGCAAATATGACATCTTGGCCTCAGAGGAT
GTAGAAGGTCAGGAGGCAGCCACACTCCCCAGCGAGGGGGGTGTTCGGATCACACCCTTTAACCTGCAGG
AGGAGATGGAGGAAGGCCACTTTGATGCCGATGGCAACTACTTCCTGAACCGGGATGCTCAGATCCGAGA
CAGCTGGCTGGACAACATTGACTGGGTGAAGATCCGGGAGCGGCCACCTGGCCAGCGCCAGGCCTCAGAC
TCGGAGGAGGAGGACAGCTTGGGCCAGACCTCAATGAGTGCCCAAGCCCTCTTGGAGGGACTTTTGGAGC
TCCTATTGCCTAGAGAGACAGTGGCTGGGGCACTGAGGCGTCTGGGGGCCCGAGGAGGAGGCAAAGGGAG
AAAGGGGCCTGGGCAACCCAGTTCCCCTCAGCGCCTGGACCGGCTCTCCGGGTTGGCCGACCAGATGGTG
GCCCGGGGCAACCTTGGTGTGTACCAGGAAACAAGGGAACGGTTGGCTATGCGTCTGAAGGGTTTGGGGT
GTCAGACCCTAGGACCCCACAATCCCACACCCCCACCCTCCCTGGACATGTTCGCTGAGGAGTTGGCGGA
GGAGGAACTGGAGACCCCAACCCCTACCCAGAGAGGAGAAGCAGAGTCGCGGGGAGATGGTCTGGTGGAT
GTGATGTGGGAATATAAGTGGGAGAACACGGGGGATGCCGAGCTGTATGGGCCCTTCACCAGCGCCCAGA
TGCAGACCTGGGTGAGTGAAGGCTACTTCCCGGACGGTGTTTATTGCCGGAAGCTGGACCCCCCTGGTGG
TCAGTTCTACAACTCCAAACGCATTGACTTTGACCTCTACACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201352 representing NM_006110
Red=Cloning site Green=Tags(s)

MPKRKVTFQGVGDEEDEDEIIVPKKKLVDPVAGSGGPGSRFKGKHSLDSDEEEDDDDGGSSKYDILASED
VEGQEAATLPSEGGVRITPFNLQEEMEEGHFDADGNYFLNRDAQIRDSWLDNIDWVKIRERPPGQRQASD
SEEEDSLGQTSMSAQALLEGLLELLLPRETVAGALRRLGARGGGKGRKGPGQPSSPQRLDRLSGLADQMV
ARGNLGVYQETRERLAMRLKGLGCQTLGPHNPTPPPSLDMFAEELAEEELETPTPTQRGEAESRGDGLVD
VMWEYKWENTGDAELYGPFTSAQMQTWVSEGYFPDGVYCRKLDPPGGQFYNSKRIDFDLYT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006110
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006110.3
RefSeq Size 1299 bp
RefSeq ORF 1026 bp
Locus ID 10421
UniProt ID O95400, A0A024QZC1
Cytogenetics 16p11.2
Domains GYF
MW 37.5 kDa
Summary This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined. provided by RefSeq, Nov 2008
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"NM_006110" in other vectors (4)
SKU Description Size Price
RC201352L3 Lenti ORF clone of Human CD2 (cytoplasmic tail) binding protein 2 (CD2BP2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201352L4 Lenti ORF clone of Human CD2 (cytoplasmic tail) binding protein 2 (CD2BP2), transcript variant 1, mGFP tagged 10 ug
$757.00
RG201352 CD2BP2 (tGFP-tagged) - Human CD2 (cytoplasmic tail) binding protein 2 (CD2BP2), transcript variant 1 10 ug
$489.00 $657.00
SC116334 CD2BP2 (untagged)-Human CD2 (cytoplasmic tail) binding protein 2 (CD2BP2), transcript variant 1 10 ug
$457.00

Plasmid Map for RC201352

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201352
Circular map for RC201352

file-alt Citations

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