RTL8A (NM_001078172) Human Tagged ORF Clone

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SKU
RC201312
FAM127B (Myc-DDK-tagged)-Human family with sequence similarity 127, member B (FAM127B), transcript variant 1
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RTL8A
Synonyms CXX1b; FAM127B; MAR8A; SIRH6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201312 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGGTCGGGTGCAGCTGATGAAGGCCCTCCTGGCCGGGCCCCTCCGGCCCGCGGCGCGTCGCTGGA
GGAACCCGATTCCCTTTCCCGAGACGTTTGACGGAGATACCGACCGACTCCCGGAGTTCATCGTGCAGAC
GTGCTCCTACATGTTCGTGGACGAGAACACGTTCTCCAACGACGCCCTGAAGGTGACGTTCCTCATCACC
CGCCTCACGGGGCCAGCCCTGCAGTGGGTGATCCCCTACATCAGGAAGGAGAGCCCCCTGCTCAATGATT
ACCGGGGCTTCCTGGCCGAGATGAAGCGGGTCTTTGGATGGGAGGAGGACGAGGACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201312 protein sequence
Red=Cloning site Green=Tags(s)

MDGRVQLMKALLAGPLRPAARRWRNPIPFPETFDGDTDRLPEFIVQTCSYMFVDENTFSNDALKVTFLIT
RLTGPALQWVIPYIRKESPLLNDYRGFLAEMKRVFGWEEDEDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001078172
ORF Size 339 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001078172.1, NP_001071640.1
RefSeq Size 1275 bp
RefSeq ORF 342 bp
Locus ID 26071
UniProt ID Q9BWD3
Cytogenetics Xq26.3
MW 13.2 kDa
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Documents
Resources
"NM_001078172" in other vectors (4)
SKU Description Size Price
RC201312L3 Lenti ORF clone of Human family with sequence similarity 127, member B (FAM127B), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC201312L4 Lenti ORF clone of Human family with sequence similarity 127, member B (FAM127B), transcript variant 1, mGFP tagged 10 ug
$450.00
RG201312 FAM127B (tGFP-tagged) - Human family with sequence similarity 127, member B (FAM127B), transcript variant 1 10 ug
$489.00
SC320237 FAM127B (untagged)-Human family with sequence similarity 127, member B (FAM127B), transcript variant 1 10 ug
$150.00

Plasmid Map for RC201312

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Cloning scheme for RC201312

Circular map for RC201312

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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