Chk2 (CHEK2) (NM_007194) Human Tagged ORF Clone
SKU
RC201278
CHEK2 (Myc-DDK-tagged)-Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1
$758.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Chk2 |
| Synonyms | CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007194 |
| ORF Size | 1629 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007194.4 |
| RefSeq Size | 1862 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 11200 |
| UniProt ID | O96017 |
| Cytogenetics | 22q12.1 |
| Domains | FHA, pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle, p53 signaling pathway |
| MW | 60.9 kDa |
| Summary | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007194" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201278L1 | Lenti ORF clone of Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,058.00
|
|
| RC201278L2 | Lenti ORF clone of Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1, mGFP tagged | 10 ug |
$1,058.00
|
|
| RC201278L3 | Lenti ORF clone of Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,058.00
|
|
| RC201278L4 | Lenti ORF clone of Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1, mGFP tagged | 10 ug |
$1,058.00
|
|
| RG201278 | CHEK2 (tGFP-tagged) - Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1 | 10 ug |
$958.00
|
|
| SC110240 | CHEK2 (untagged)-Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1 | 10 ug |
$759.00
|
|
| SC323572 | CHEK2 (untagged)-Kinase deficient mutant (K249M) of Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1 | 10 ug |
$759.00
|
|
Citations
Tumour-intrinsic PDL1 signals regulate the Chk2 DNA damage response in cancer cells and mediate resistance to Chk1 inhibitors
Murray, Kornepati, Ontiveros et al
Mol Cancer (2024) 23 (1), 242 DOI: 10.1186/s12943-024-02147-z
Murray, Kornepati, Ontiveros et al
Mol Cancer (2024) 23 (1), 242 DOI: 10.1186/s12943-024-02147-z
Cited by 12
AI Summary: OriGene contributed Chek2 expression plasmids (RC201278 for human and MR208692 for mouse) used in Chk2 re-expression experiments to investigate the role of tumour-intrinsic PDL1 in regulating the DNA damage response in cancer cells.
Snippet: munoblotting and/or sequencing. Plasmids, transfections, and lentivirus transductions Chek2 expression plasmids or vector only controls for Chk2 re-expression experiments (Chk2RE) were obtained from OriGene (RC201278 and MR208692 for human and mouse, respectively). RCHY1 (PIRH2) siRNA Smartpools or scrambled controls were obtained from Dharmacon (Cat#: L-065323–01-0005). Mouse PDL1 expression p
RA-Induced Transcriptional Silencing of Checkpoint Kinase-2 through Promoter Methylation by Dnmt3b Is Required for Neuronal Differentiation of P19 Cells
Sheikh, Malik, Zhu
J. Mol. Biol. (2017)
Sheikh, Malik, Zhu
J. Mol. Biol. (2017)
AI Summary: In this study, OriGene's pCMV and pCMV-Chk2 plasmids were used to transfect P19 cells and investigate the effects of enforced Chk2 expression on neuronal differentiation.
Snippet: Briefly, 3 × 10 5 P19 cells were plated per 60 mm dish one day before transfection. Next day, the monolayer cells were transfected by mixing plasmid DNA (pCMV and pCMV-Chk2, both from Origene) with Fugene6 in serum free media. ... All 2 versions Cite SaveSaving
Showing 1-2 of 2 papers.
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