PPIC (NM_000943) Human Tagged ORF Clone

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SKU
RC201143
PPIC (Myc-DDK-tagged)-Human peptidylprolyl isomerase C (cyclophilin C) (PPIC)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPIC
Synonyms CYPC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201143 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCCGGGTCCTCGGCTGCTGCTACCTCTCGTGCTTTGCGTGGGGCTCGGCGCACTTGTGTTTTCTT
CGGGGGCCGAGGGCTTCCGCAAGCGAGGCCCCTCGGTGACGGCCAAGGTCTTCTTTGATGTGAGGATTGG
AGACAAAGATGTTGGCAGAATTGTGATTGGCCTCTTTGGAAAAGTTGTGCCCAAGACAGTGGAAAATTTT
GTTGCTCTAGCAACAGGAGAGAAAGGATATGGATATAAAGGAAGCAAGTTTCATCGTGTCATCAAGGATT
TCATGATTCAAGGAGGTGACATCACCACTGGAGATGGCACTGGGGGTGTGAGCATCTATGGTGAGACATT
TCCAGATGAGAACTTCAAGCTGAAGCACTATGGCATTGGGTGGGTCAGCATGGCCAACGCTGGGCCTGAC
ACCAATGGCTCTCAGTTCTTTATCACCTTGACCAAGCCCACCTGGTTGGACGGCAAACATGTGGTGTTTG
GAAAAGTCATTGATGGGATGACAGTGGTGCACTCCATAGAGCTCCAAGCAACTGATGGGCATGACCGTCC
ACTCACCAACTGCTCGATCATCAACAGTGGCAAGATAGACGTGAAAACGCCTTTTGTGGTTGAGATCGCT
GATTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201143 protein sequence
Red=Cloning site Green=Tags(s)

MGPGPRLLLPLVLCVGLGALVFSSGAEGFRKRGPSVTAKVFFDVRIGDKDVGRIVIGLFGKVVPKTVENF
VALATGEKGYGYKGSKFHRVIKDFMIQGGDITTGDGTGGVSIYGETFPDENFKLKHYGIGWVSMANAGPD
TNGSQFFITLTKPTWLDGKHVVFGKVIDGMTVVHSIELQATDGHDRPLTNCSIINSGKIDVKTPFVVEIA
DW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000943
ORF Size 636 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000943.5
RefSeq Size 1241 bp
RefSeq ORF 639 bp
Locus ID 5480
UniProt ID P45877
Cytogenetics 5q23.2
Domains pro_isomerase
Protein Families Transmembrane
MW 22.8 kDa
Summary The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase)) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000943" in other vectors (4)
SKU Description Size Price
RC201143L3 Lenti ORF clone of Human peptidylprolyl isomerase C (cyclophilin C) (PPIC), Myc-DDK-tagged 10 ug
$600.00
RC201143L4 Lenti ORF clone of Human peptidylprolyl isomerase C (cyclophilin C) (PPIC), mGFP tagged 10 ug
$600.00
RG201143 PPIC (tGFP-tagged) - Human peptidylprolyl isomerase C (cyclophilin C) (PPIC) 10 ug
$500.00
SC319090 PPIC (untagged)-Human peptidylprolyl isomerase C (cyclophilin C) (PPIC) 10 ug
$300.00

Plasmid Map for RC201143

Download sequence for

Cloning scheme for RC201143

Circular map for RC201143

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