GLI1 (NM_005269) Human Tagged ORF Clone
- TrueORF®
SKU
RC201110
GLI1 (Myc-DDK-tagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 1
$1,444.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GLI1 |
| Synonyms | GLI; PAPA8; PPD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005269 |
| ORF Size | 3318 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005269.3 |
| RefSeq Size | 3618 bp |
| RefSeq ORF | 3321 bp |
| Locus ID | 2735 |
| UniProt ID | P08151 |
| Cytogenetics | 12q13.3 |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| MW | 117.9 kDa |
| Summary | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005269" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201110L1 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,744.00
|
|
| RC201110L2 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, mGFP tagged | 10 ug |
$1,744.00
|
|
| RC201110L3 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,744.00
|
|
| RC201110L4 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, mGFP tagged | 10 ug |
$1,744.00
|
|
| RG201110 | GLI1 (tGFP-tagged) - Human GLI family zinc finger 1 (GLI1), transcript variant 1 | 10 ug |
$1,644.00
|
|
| SC125780 | GLI1 (untagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 1 | 10 ug |
$1,445.00
|
|
Citations
Hedgehog signalling is involved in acquired resistance to KRASG12C inhibitors in lung cancer cells
Lee, Yi, Park et al
Cell Death Dis (2024) 15 (1), 56 DOI: 10.1038/s41419-024-06436-9
Lee, Yi, Park et al
Cell Death Dis (2024) 15 (1), 56 DOI: 10.1038/s41419-024-06436-9
Cited by 9
AI Summary: OriGene’s pCMV6-Entry GLI1 plasmid (RC201110) was used in this study to ectopically express GLI1, enabling the researchers to investigate the role of GLI1 in mediating KRAS re-expression and acquired resistance to KRASG12C inhibitors in lung cancer cells.
Snippet: uroraA GFP-AURKA-GFP (deposited by Marc Tramier, Institute Genetics & Development of Rennes, Rennes, France) was purchased from Addgene (#157765, addgene.org). The pCMV6-Entry GLI1 was purchased from Origene (RC201110, Rockville, MD, USA). The KRAS promoter reporter pEZX-PG04-KRAS was purchased from GeneCopoeia (HPRM45839-PG04, Rockville, MD, USA). Cells were transfected with plasmid vectors using
Hedgehog/GLI1 Transcriptionally Regulates FANCD2 in Ovarian Tumor Cells: Its Inhibition Induces HR-Deficiency and Synergistic Lethality with PARP Inhibition
Mani, Tripathi, Chaudhary et al
Neoplasia (2021) 23 (9), 1002-1015 DOI: 10.1016/j.neo.2021.06.010
Mani, Tripathi, Chaudhary et al
Neoplasia (2021) 23 (9), 1002-1015 DOI: 10.1016/j.neo.2021.06.010
Cited by 17
AI Summary: In this study, the OriGene GLI1 (Myc-tagged) plasmid (Cat No: RC201110) was used to overexpress GLI1 in ovarian tumor cells, enabling the investigation of GLI1's role in regulating FANCD2 and its impact on DNA repair and sensitivity to PARP inhibition.
Snippet: UCCACUCU) [10]. For GLI1 down-regulation, the data using siGLI1-5 sequences were presented in these studies unless otherwise mentioned. GLI1 (Myc- tagged) plasmid (Cat No: RC201110) was procured from Origene and transfected using Lipofectamine 2000 according to the manufacturer's protocols. Colony assay For experiments using siRNA knockdown, 48 hours after siGLI1 or siControl transfection, cells
The stem cell-associated transcription co-factor, ZNF521, interacts with GLI1 and GLI2 and enhances the activity of the Sonic hedgehog pathway
Scicchitano, Giordano, Lucchino et al
Cell Death Dis (2019) 10 (10), 715 DOI: 10.1038/s41419-019-1946-x
Scicchitano, Giordano, Lucchino et al
Cell Death Dis (2019) 10 (10), 715 DOI: 10.1038/s41419-019-1946-x
AI Summary: OriGene contributed to this study by providing the TrueORF Gold human ZNF521 (pCMV6-ZNF521-Myc-DDK) expression plasmid, which was used to investigate the role of ZNF521 in enhancing Sonic hedgehog pathway activity.
Snippet: ,39 as well as TrueORF-Gold human {"type":"entrez-nucleotide","attrs":{"text":"NM_015461.2","term_id":"219801668","term_text":"NM_015461.2"}}NM_015461.2 [/nuccore/NM_015461.2] pCMV6-_ZNF521_-Myc-DDK (Origene) and the mouse cDNA clones pCMV-3xHA-_Zfp521_37, Image Clone pCMV-Sport6-_Zfp521_ (_Evi3_) {"type":"entrez-nucleotide","attrs":{"text":"NM_145492.4","term_id":"293651570","term_text":"NM_14549;;... ,39 as well as TrueORF-Gold human {"type":"entrez-nucleotide","attrs":{"text":"NM_015461.2","term_id":"219801668","term_text":"NM_015461.2"}}NM_015461.2 [/nuccore/NM_015461.2] pCMV6-_ZNF521_-Myc-DDK (Origene) and the mouse cDNA clones pCMV-3xHA-_Zfp521_37, Image Clone pCMV-Sport6-_Zfp521_ (_Evi3_) {"type":"entrez-nucleotide","attrs":{"text":"NM_145492.4","term_id":"293651570","term_text":"NM_14549
Trim32 suppresses cerebellar development and tumorigenesis by degrading Gli1/sonic hedgehog signaling
Wang, Luo, Zhang et al
Cell Death Differ. (2019)
Wang, Luo, Zhang et al
Cell Death Differ. (2019)
Data on peptides identified by mass spectrometry analysis of in vitro DYRK1A-mediated phosphorylation sites on GLI1
Ehe, Lamson, Tarpley et al
Data Brief (2017) 15, 577-583 DOI: 10.1016/j.dib.2017.09.057
Ehe, Lamson, Tarpley et al
Data Brief (2017) 15, 577-583 DOI: 10.1016/j.dib.2017.09.057
Snippet: …GLI1 protein expression and purification. Recombinant human GLI1 protein was produced by transfection of the pCMV6 GLI1-myc-DDK plasmid (Origene, Rockville, MD) into HEK-293 cells (CRL-1573;ATCC, Manassas ...;... 2. … GLI1 protein expression and purification. Recombinant human GLI1 protein was produced by transfection of the pCMV6 GLI1-myc-DDK plasmid (Origene, Rockville, MD) into HEK-293 cells (CRL-1573;ATCC, Manassas
Identification of a DYRK1A-mediated phosphorylation site within the nuclear localization sequence of the hedgehog transcription factor GLI1
Ehe, Lamson, Tarpley et al
Biochem. Biophys. Res. Commun. (2017)
Ehe, Lamson, Tarpley et al
Biochem. Biophys. Res. Commun. (2017)
AI Summary: OriGene contributed to this study by providing the human recombinant GLI1 protein (#TP301110) and the GLI1 expression vector (#RC201110), which were essential for investigating DYRK1A-mediated phosphorylation of GLI1.
Snippet: …Human recombinant GLI1 protein (#TP301110;C-terminal MYC/DDK-tagged) and expression vector (#RC201110;pCMV6 vector containing C-terminal myc-DDK tagged-GLI1) were from Origene (Rockville, MD). ... …Human recombinant GLI1 protein (#TP301110;pCMV6 vector containing C-terminal myc-DDK tagged-GLI1) were from Origene (Rockville, MD). ... …
Showing 1-6 of 8 papers.
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