SERTAD1 (NM_013376) Human Tagged ORF Clone

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SKU
RC201064
SERTAD1 (Myc-DDK-tagged)-Human SERTA domain containing 1 (SERTAD1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SERTAD1
Synonyms SEI1; TRIP-Br1; TRIPBR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201064 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGCAAGGGTCTGAAGCGGAAACGGGAGGAGGAGGAGGAGAAGGAACCTCTGGCAGTCGACTCCT
GGTGGCTAGATCCTGGCCACACAGCGGTGGCACAGGCACCCCCGGCCGTGGCCTCTAGCTCCCTCTTTGA
CCTCTCAGTGCTCAAGCTCCACCACAGCCTGCAGCAGAGTGAGCCGGACCTGCGGCACCTGGTGCTGGTC
GTGAACACTCTGCGGCGCATCCAGGCGTCCATGGCACCCGCGGCTGCCCTGCCACCTGTGCCTAGCCCAC
CTGCAGCCCCCAGTGTGGCTGACAACTTACTGGCAAGCTCGGACGCTGCCCTTTCAGCCTCCATGGCCAG
CCTCCTGGAGGACCTCAGCCACATTGAGGGCCTGAGTCAGGCTCCCCAACCCTTGGCAGACGAGGGGCCA
CCAGGCCGTAGCATCGGGGGAGCAGCGCCCAGCCTGGGTGCCTTGGACCTGCTGGGCCCAGCCACTGGCT
GTCTACTGGACGATGGGCTTGAGGGCCTGTTTGAGGATATTGACACCTCTATGTATGACAATGAACTTTG
GGCACCAGCCTCTGAGGGCCTCAAACCAGGCCCTGAGGATGGGCCGGGCAAGGAGGAAGCTCCGGAGCTG
GACGAGGCCGAATTGGACTACCTCATGGATGTGCTGGTGGGCACACAGGCACTGGAGCGACCGCCGGGGC
CAGGGCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201064 protein sequence
Red=Cloning site Green=Tags(s)

MLSKGLKRKREEEEEKEPLAVDSWWLDPGHTAVAQAPPAVASSSLFDLSVLKLHHSLQQSEPDLRHLVLV
VNTLRRIQASMAPAAALPPVPSPPAAPSVADNLLASSDAALSASMASLLEDLSHIEGLSQAPQPLADEGP
PGRSIGGAAPSLGALDLLGPATGCLLDDGLEGLFEDIDTSMYDNELWAPASEGLKPGPEDGPGKEEAPEL
DEAELDYLMDVLVGTQALERPPGPGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013376
ORF Size 708 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013376.4
RefSeq Size 1213 bp
RefSeq ORF 711 bp
Locus ID 29950
UniProt ID Q9UHV2
Cytogenetics 19q13.2
Protein Families Transcription Factors
MW 24.7 kDa
Summary Acts at E2F-responsive promoters as coregulator to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F1/TFDP1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of CDKN2A/p16INK4A.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013376" in other vectors (6)
SKU Description Size Price
RC201064L1 Lenti ORF clone of Human SERTA domain containing 1 (SERTAD1), Myc-DDK-tagged 10 ug
$600.00
RC201064L2 Lenti ORF clone of Human SERTA domain containing 1 (SERTAD1), mGFP tagged 10 ug
$600.00
RC201064L3 Lenti ORF clone of Human SERTA domain containing 1 (SERTAD1), Myc-DDK-tagged 10 ug
$600.00
RC201064L4 Lenti ORF clone of Human SERTA domain containing 1 (SERTAD1), mGFP tagged 10 ug
$600.00
RG201064 SERTAD1 (tGFP-tagged) - Human SERTA domain containing 1 (SERTAD1) 10 ug
$489.00 $500.00
SC320059 SERTAD1 (untagged)-Human SERTA domain containing 1 (SERTAD1) 10 ug
$300.00

Plasmid Map for RC201064

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Cloning scheme for RC201064

Circular map for RC201064

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