PSMC3IP (NM_013290) Human Tagged ORF Clone

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SKU
RC201045
PSMC3IP (Myc-DDK-tagged)-Human PSMC3 interacting protein (PSMC3IP), transcript variant 1
$289.00 $300.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PSMC3IP
Synonyms GT198; HOP2; HUMGT198A; ODG3; TBPIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201045 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTAAAGGCCGGGCAGAAGCTGCGGCGGGAGCCGCCGGGATCCTCCTGAGGTACCTGCAGGAGCAGA
ACCGGCCCTACAGCTCCCAGGATGTGTTCGGGAACCTACAGCGGGAACACGGACTGGGCAAGGCGGTGGT
GGTGAAGACGCTGGAGCAGCTGGCGCAACAAGGCAAGATCAAAGAGAAGATGTACGGCAAGCAGAAGATC
TATTTTGCGGATCAGGACCAGTTTGACATGGTGAGTGATGCTGACCTTCAAGTCCTAGATGGCAAAATCG
TGGCCCTCACTGCTAAGGTGCAGAGCTTGCAGCAGAGCTGCCGCTACATGGAGGCTGAGATGCAGAAAGA
AATCCAGGAGTTAAAGAAGGAATGCGCTGGCTACAGAGAGAGATTGAAGAACATTAAAGCAGCTACCAAT
CATGTGACTCCAGAAGAGAAAGAGCAGGTGTACAGAGAGAGGCAGAAGTACTGTAAGGAGTGGAGGAAGA
GGAAGAGGATGGCTACAGAGCTGTCTGATGCAATACTTGAAGGATACCCCAAGAGCAAGAAGCAGTTCTT
TGAGGAAGTTGGGATAGAGACGGATGAAGATTACAACGTCACACTCCCAGACCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201045 protein sequence
Red=Cloning site Green=Tags(s)

MSKGRAEAAAGAAGILLRYLQEQNRPYSSQDVFGNLQREHGLGKAVVVKTLEQLAQQGKIKEKMYGKQKI
YFADQDQFDMVSDADLQVLDGKIVALTAKVQSLQQSCRYMEAEMQKEIQELKKECAGYRERLKNIKAATN
HVTPEEKEQVYRERQKYCKEWRKRKRMATELSDAILEGYPKSKKQFFEEVGIETDEDYNVTLPDP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013290
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013290.6, NP_037422.2
RefSeq Size 1440 bp
RefSeq ORF 618 bp
Locus ID 29893
UniProt ID Q9P2W1
Cytogenetics 17q21.2
Protein Families Druggable Genome
MW 23.6 kDa
Summary This gene encodes a protein that functions in meiotic recombination. It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. The protein encoded by this gene can also co-activate ligand-driven transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in this gene cause XX female gonadal dysgenesis. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Dec 2011
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Documents
Resources
"NM_013290" in other vectors (4)
SKU Description Size Price
RC201045L3 Lenti ORF clone of Human PSMC3 interacting protein (PSMC3IP), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC201045L4 Lenti ORF clone of Human PSMC3 interacting protein (PSMC3IP), transcript variant 1, mGFP tagged 10 ug
$600.00
RG201045 PSMC3IP (tGFP-tagged) - Human PSMC3 interacting protein (PSMC3IP), transcript variant 1 10 ug
$500.00
SC309418 PSMC3IP (untagged)-Human PSMC3 interacting protein (PSMC3IP), transcript variant 1 10 ug
$330.00

Plasmid Map for RC201045

Download sequence for

Cloning scheme for RC201045

Circular map for RC201045

file-alt Citations

GDF15 inhibits early-stage adipocyte differentiation by enhancing HOP2 expression and suppressing C/EBPα expression
Kim, Kang, Kim et al
Mol Cell Endocrinol (2025) 598, 112461 DOI: 10.1016/j.mce.2025.112461
Cited by 9
AI Summary: OriGene products, specifically the PSMC3IP (HOP2: RC201045; Myc-DDK-tagged) and GDF15 (RG201295; tGFP-tagged) plasmids, were used in this study to overexpress HOP2 and GDF15 in cells, enabling the investigation of their roles in adipocyte differentiation.
Snippet: … The following plasmids were purchased from OriGene (Rockville, MD, USA): PSMC3IP (HOP2: RC201045;Myc-DDK-tagged) and GDF15 (RG201295;tGFP-tagged). …
GDF15 inhibits early-stage adipocyte differentiation by enhancing HOP2 expression and suppressing C/EBPα expression
Kim, Kang, Kim et al
Research Square (2024)
Snippet: NTERFERENCE Transient transfection was carried out using Lipofectamine 2000 reagent (11668019;Thermo Fisher) following the manufacturer's guidelines. The following plasmids were purchased from OriGene (Rockville, MD, USA): PSMC3IP (HOP2;RC201045;Myc-DDK-tagged) and GDF15 (RG201295;tGFP-tagged). For RNA interference, the cells were transfected with 100 pmol siRNA using Lipofectamine RNA
Showing 1-2 of 2 papers.
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