TRIAP1 (NM_016399) Human Tagged ORF Clone

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SKU
RC201032
TRIAP1 (Myc-DDK-tagged)-Human TP53 regulated inhibitor of apoptosis 1 (TRIAP1)
  $225.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRIAP1
Synonyms HSPC132; MDM35; P53CSV; WF-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201032 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGTGTGGGGGAGGCATGCACGGACATGAAGCGCGAGTACGACCAGTGCTTCAATCGCTGGTTCG
CCGAGAAATTTCTCAAGGGGGACAGCTCCGGGGACCCGTGCACCGACCTCTTCAAGCGCTACCAGCAGTG
TGTTCAGAAAGCAATAAAGGAGAAAGAGATTCCTATTGAAGGACTGGAGTTCATGGGCCATGGCAAAGAA
AAGCCTGAAAATTCTTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201032 protein sequence
Red=Cloning site Green=Tags(s)

MNSVGEACTDMKREYDQCFNRWFAEKFLKGDSSGDPCTDLFKRYQQCVQKAIKEKEIPIEGLEFMGHGKE
KPENSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016399
ORF Size 228 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016399.3
RefSeq Size 1196 bp
RefSeq ORF 231 bp
Locus ID 51499
UniProt ID O43715
Cytogenetics 12q24.31
MW 8.8 kDa
Summary Involved in the modulation of the mitochondrial apoptotic pathway by ensuring the accumulation of cardiolipin (CL) in mitochondrial membranes. In vitro, the TRIAP1:PRELID1 complex mediates the transfer of phosphatidic acid (PA) between liposomes and probably functions as a PA transporter across the mitochondrion intermembrane space to provide PA for CL synthesis in the inner membrane (PubMed:23931759). Likewise, the TRIAP1:PRELID3A complex mediates the transfer of phosphatidic acid (PA) between liposomes (in vitro) and probably functions as a PA transporter across the mitochondrion intermembrane space (in vivo) (PubMed:26071602). Mediates cell survival by inhibiting activation of caspase-9 which prevents induction of apoptosis (PubMed:15735003).UniProtKB/Swiss-Prot Function
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Documents
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"NM_016399" in other vectors (6)
SKU Description Size Price
RC201032L1 Lenti ORF clone of Human TP53 regulated inhibitor of apoptosis 1 (TRIAP1), Myc-DDK-tagged 10 ug
$525.00
RC201032L2 Lenti ORF clone of Human TP53 regulated inhibitor of apoptosis 1 (TRIAP1), mGFP tagged 10 ug
$525.00
RC201032L3 Lenti ORF clone of Human TP53 regulated inhibitor of apoptosis 1 (TRIAP1), Myc-DDK-tagged 10 ug
$525.00
RC201032L4 Lenti ORF clone of Human TP53 regulated inhibitor of apoptosis 1 (TRIAP1), mGFP tagged 10 ug
$525.00
RG201032 TRIAP1 (tGFP-tagged) - Human TP53 regulated inhibitor of apoptosis 1 (TRIAP1) 10 ug
$425.00
SC122844 TRIAP1 (untagged)-Human TP53 regulated inhibitor of apoptosis 1 (TRIAP1) 10 ug
$225.00

Plasmid Map for RC201032

Download sequence for

Cloning scheme for RC201032

Circular map for RC201032

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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