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COPZ1 (NM_016057) Human Tagged ORF Clone

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SKU
RC201023
COPZ1 (Myc-DDK-tagged)-Human coatomer protein complex, subunit zeta 1 (COPZ1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COPZ1
Synonyms CGI-120; COPZ; HSPC181; zeta-COP; zeta1-COP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201023 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGCTGATTTTGGAACCTTCCCTGTATACTGTCAAAGCCATCCTGATTCTGGACAATGATGGAG
ATCGACTTTTTGCCAAGTACTATGACGACACCTACCCCAGTGTCAAGGAGCAAAAGGCCTTTGAGAAGAA
CATTTTCAACAAGACCCATCGGACTGACAGTGAAATTGCCCTCTTGGAAGGCCTGACAGTGGTATACAAA
AGCAGTATAGATCTCTATTTCTATGTGATTGGCAGCTCCTATGAAAATGAGCTGATGCTTATGGCTGTTC
TGAACTGTCTCTTCGACTCATTGAGCCAGATGCTGAGGAAAAATGTAGAAAAGCGAGCACTGCTGGAGAA
CATGGAGGGGCTGTTCTTGGCTGTGGATGAAATTGTAGATGGAGGGGTGATCCTAGAGAGTGATCCCCAG
CAGGTGGTACACCGGGTGGCATTAAGGGGTGAAGATGTCCCCCTTACGGAGCAGACCGTGTCTCAGGTGC
TGCAGTCAGCCAAAGAACAGATCAAGTGGTCACTCCTTCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201023 protein sequence
Red=Cloning site Green=Tags(s)

MEALILEPSLYTVKAILILDNDGDRLFAKYYDDTYPSVKEQKAFEKNIFNKTHRTDSEIALLEGLTVVYK
SSIDLYFYVIGSSYENELMLMAVLNCLFDSLSQMLRKNVEKRALLENMEGLFLAVDEIVDGGVILESDPQ
QVVHRVALRGEDVPLTEQTVSQVLQSAKEQIKWSLLR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016057
ORF Size 531 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016057.3
RefSeq Size 1954 bp
RefSeq ORF 534 bp
Locus ID 22818
UniProt ID P61923
Cytogenetics 12q13.13
Domains Clat_adaptor_s
MW 20.2 kDa
Summary This gene encodes a subunit of the cytoplasmic coatamer protein complex, which is involved in autophagy and intracellular protein trafficking. The coatomer protein complex is comprised of seven subunits and functions as the coat protein of coat protein complex (COP)I-vesicles. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2012
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"NM_016057" in other vectors (4)
SKU Description Size Price
RC201023L3 Lenti ORF clone of Human coatomer protein complex, subunit zeta 1 (COPZ1), Myc-DDK-tagged 10 ug
$600.00
RC201023L4 Lenti ORF clone of Human coatomer protein complex, subunit zeta 1 (COPZ1), mGFP tagged 10 ug
$600.00
RG201023 COPZ1 (tGFP-tagged) - Human coatomer protein complex, subunit zeta 1 (COPZ1) 10 ug
$500.00
SC321396 COPZ1 (untagged)-Human coatomer protein complex, subunit zeta 1 (COPZ1) 10 ug
$300.00

Plasmid Map for RC201023

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201023
Circular map for RC201023

file-alt Citations

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