OLFML3 (NM_020190) Human Tagged ORF Clone

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SKU
RC200923
OLFML3 (Myc-DDK-tagged)-Human olfactomedin-like 3 (OLFML3)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OLFML3
Synonyms HNOEL-iso; OLF44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200923 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCCAGCACCCCTCTCCTCATCTTGTTCCTTTTGTCATGGTCGGGACCCCTCCAAGGACAGCAGC
ACCACCTTGTGGAGTACATGGAACGCCGACTAGCTGCTTTAGAGGAACGGCTGGCCCAGTGCCAGGACCA
GAGTAGTCGGCATGCTGCTGAGCTGCGGGACTTCAAGAACAAGATGCTGCCACTGCTGGAGGTGGCAGAG
AAGGAGCGGGAGGCACTCAGAACTGAGGCCGACACCATCTCCGGGAGAGTGGATCGTCTGGAGCGGGAGG
TAGACTATCTGGAGACCCAGAACCCAGCTCTGCCCTGTGTAGAGTTTGATGAGAAGGTGACTGGAGGCCC
TGGGACCAAAGGCAAGGGAAGAAGGAATGAGAAGTACGATATGGTGACAGACTGTGGCTACACAATCTCT
CAAGTGAGATCAATGAAGATTCTGAAGCGATTTGGTGGCCCAGCTGGTCTATGGACCAAGGATCCACTGG
GGCAAACAGAGAAGATCTACGTGTTAGATGGGACACAGAATGACACAGCCTTTGTCTTCCCAAGGCTGCG
TGACTTCACCCTTGCCATGGCTGCCCGGAAAGCTTCCCGAGTCCGGGTGCCCTTCCCCTGGGTAGGCACA
GGGCAGCTGGTATATGGTGGCTTTCTTTATTTTGCTCGGAGGCCTCCTGGAAGACCTGGTGGAGGTGGTG
AGATGGAGAACACTTTGCAGCTAATCAAATTCCACCTGGCAAACCGAACAGTGGTGGACAGCTCAGTATT
CCCAGCAGAGGGGCTGATCCCCCCCTACGGCTTGACAGCAGACACCTACATCGACCTGGCAGCTGATGAG
GAAGGTCTTTGGGCTGTCTATGCCACCCGGGAGGATGACAGGCACTTGTGTCTGGCCAAGTTAGATCCAC
AGACACTGGACACAGAGCAGCAGTGGGACACACCATGTCCCAGAGAGAATGCTGAGGCTGCCTTTGTCAT
CTGTGGGACCCTCTATGTCGTCTATAACACCCGTCCTGCCAGTCGGGCCCGCATCCAGTGCTCCTTTGAT
GCCAGCGGCACCCTGACCCCTGAACGGGCAGCACTCCCTTATTTTCCCCGCAGATATGGTGCCCATGCCA
GCCTCCGCTATAACCCCCGAGAACGCCAGCTCTATGCCTGGGATGATGGCTACCAGATTGTCTATAAGCT
GGAGATGAGGAAGAAAGAGGAGGAGGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200923 protein sequence
Red=Cloning site Green=Tags(s)

MGPSTPLLILFLLSWSGPLQGQQHHLVEYMERRLAALEERLAQCQDQSSRHAAELRDFKNKMLPLLEVAE
KEREALRTEADTISGRVDRLEREVDYLETQNPALPCVEFDEKVTGGPGTKGKGRRNEKYDMVTDCGYTIS
QVRSMKILKRFGGPAGLWTKDPLGQTEKIYVLDGTQNDTAFVFPRLRDFTLAMAARKASRVRVPFPWVGT
GQLVYGGFLYFARRPPGRPGGGGEMENTLQLIKFHLANRTVVDSSVFPAEGLIPPYGLTADTYIDLAADE
EGLWAVYATREDDRHLCLAKLDPQTLDTEQQWDTPCPRENAEAAFVICGTLYVVYNTRPASRARIQCSFD
ASGTLTPERAALPYFPRRYGAHASLRYNPRERQLYAWDDGYQIVYKLEMRKKEEEV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020190
ORF Size 1218 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020190.5
RefSeq Size 1854 bp
RefSeq ORF 1221 bp
Locus ID 56944
UniProt ID Q9NRN5, M1LAK4
Cytogenetics 1p13.2
Domains OLF
Protein Families Secreted Protein
MW 46 kDa
Summary This gene encodes a member of the olfactomedin-like gene family which also includes genes encoding noelin, tiarin, myocilin, amassin, optimedin, photomedin, and latrophilin. The encoded protein is a secreted extracellular matrix glycoprotein with a C-terminal olfactomedin domain that facilitates protein-protein interactions, cell adhesion, and intercellular interactions. It serves as both a scaffold protein that recruits bone morphogenetic protein 1 to its substrate chordin, and as a vascular tissue remodeler with pro-angiogenic properties. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2017
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Documents
Resources
"NM_020190" in other vectors (4)
SKU Description Size Price
RC200923L3 Lenti ORF clone of Human olfactomedin-like 3 (OLFML3), Myc-DDK-tagged 10 ug
$757.00
RC200923L4 Lenti ORF clone of Human olfactomedin-like 3 (OLFML3), mGFP tagged 10 ug
$757.00
RG200923 OLFML3 (tGFP-tagged) - Human olfactomedin-like 3 (OLFML3) 10 ug
$489.00 $657.00
SC320863 OLFML3 (untagged)-Human olfactomedin-like 3 (OLFML3) 10 ug
$457.00

Plasmid Map for RC200923

Download sequence for

Cloning scheme for RC200923

Circular map for RC200923

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