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CBARA1 (MICU1) (NM_006077) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200921
MICU1 (Myc-DDK-tagged)-Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CBARA1 or NM_006077"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CBARA1
Synonyms ara CALC; CALC; CBARA1; EFHA3; MPXPS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200921 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCGTCTGAACTCACTTTCTGCTTTGGCAGAACTGGCTGTGGGTTCTCGATGGTACCATGGAGGAT
CACAGCCCATCCAGATCCGGCGAAGACTAATGATGGTGGCTTTCCTGGGAGCATCTGCAGTAACTGCAAG
TACTGGTCTTTTGTGGAAGAGGGCCCATGCAGAATCTCCACCATGTGTAGACAACCTAAAAAGTGACATC
GGTGATAAAGGGAAGAATAAAGATGAAGGGGATGTTTGTAACCATGAGAAAAAGACTGCAGATCTTGCCC
CTCACCCAGAAGAGAAAAAGAAGAAACGTTCTGGATTCAGAGACAGAAAAGTGATGGAATATGAGAATAG
GATTCGAGCCTACTCCACGCCAGACAAAATCTTCCGATATTTTGCCACCTTGAAAGTCATCAGTGAGCCT
GGTGAAGCAGAAGTGTTTATGACACCAGAAGATTTTGTGCGATCCATAACACCCAATGAAAAACAACCAG
AACACTTGGGTCTGGATCAATATATAATAAAACGCTTTGATGGAAAGAAAATTTCCCAGGAACGAGAAAA
ATTTGCTGATGAAGGCAGTATATTTTACACCCTTGGAGAATGTGGGCTCATATCCTTTTCAGACTACATT
TTCCTCACAACTGTTCTTTCCACTCCTCAGAGAAATTTTGAAATTGCCTTCAAGATGTTTGATTTGAATG
GAGATGGAGAAGTAGATATGGAAGAATTTGAACAGGTTCAGAGCATCATTCGCTCCCAAACCAGTATGGG
TATGCGCCACAGAGATCGTCCAACTACTGGCAACACCCTCAAGTCTGGCTTGTGTTCAGCCCTCACAACC
TACTTTTTTGGAGCTGATCTGAAGGGAAAGCTGACAATCAAAAACTTCCTCGAATTTCAGCGTAAACTGC
AGCATGATGTTCTGAAGCTTGAGTTTGAACGCCATGACCCTGTGGATGGGAGAATTACTGAGAGGCAGTT
TGGTGGCATGCTACTTGCCTACAGTGGGGTGCAGTCCAAGAAGCTGACCGCCATGCAGAGGCAGCTCAAG
AAGCACTTCAAAGAAGGAAAGGGTCTGACATTTCAGGAGGTGGAGAACTTCTTTACTTTCCTAAAGAACA
TTAATGATGTGGACACTGCATTGAGTTTTTACCATATGGCTGGAGCATCTCTTGATAAAGTGACCATGCA
GCAGGTGGCCAGGACAGTGGCTAAAGTGGAGCTCTCAGACCACGTGTGTGATGTGGTGTTTGCACTCTTT
GACTGTGATGGCAATGGCGAACTGAGCAATAAGGAATTTGTTTCCATCATGAAGCAACGGCTGATGAGAG
GCCTGGAAAAGCCCAAAGACATGGGTTTCACTCGCCTCATGCAGGCCATGTGGAAATGTGCACAGGAAAC
TGCCTGGGACTTCGCTTTACCCAAACAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200921 protein sequence
Red=Cloning site Green=Tags(s)

MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDI
GDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEP
GEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSDYI
FLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTT
YFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLK
KHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALF
DCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006077
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006077.3
RefSeq Size 2506 bp
RefSeq ORF 1437 bp
Locus ID 10367
UniProt ID Q9BPX6
Cytogenetics 10q22.1
Domains EFh
MW 54.4 kDa
Summary This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Mar 2013
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Documents
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"NM_006077" in other vectors (6)
SKU Description Size Price
RC200921L1 Lenti ORF clone of Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200921L2 Lenti ORF clone of Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$757.00
RC200921L3 Lenti ORF clone of Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200921L4 Lenti ORF clone of Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$757.00
RG200921 MICU1 (tGFP-tagged) - Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00 $657.00
SC111147 MICU1 (untagged)-Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$457.00

Plasmid Map for RC200921

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200921
Circular map for RC200921

file-alt Citations

Strongly coupled transmembrane mechanisms control MCU-mediated mitochondrial Ca2+ uptake
Vais, Payne, Li et al
bioRxiv (2020)
Cited by 2
Snippet: MCU-KO, MICU1-KO and MICU2-KO HEK-293T cells were a generous gift from Dr. Vamsi Mootha. Full length MICU1, MICU2 and EMRE cDNAs were purchased from Origene Technologies USA. EMRE-ΔC-V5 constructs were generated by a PCR-based cloning strategy as described (23)
EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter
Vais, Mallilankaraman, Mak et al
Cell Rep (2016) 14 (3), 403-10 DOI: 10.1016/j.celrep.2015.12.054
Cited by 154
AI Summary: OriGene products contributed to this study by providing full-length MICU1, MICU2, and EMRE cDNAs, which were essential for investigating the molecular mechanisms of mitochondrial Ca(2+) uniporter regulation.
Snippet: Mallilankaraman et al., 2012). Full-length MICU1, MICU2, and EMRE cDNAs were purchased from Origene Technologies. EMRE-ΔC-V5 and EMRE-QN6-V5 constructs were generated by a PCR-based cloning strategy. All constructs were ... Cite SaveSaving
Showing 1-2 of 2 papers.
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