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SIN1 (MAPKAP1) (NM_024117) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200885
MAPKAP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase associated protein 1 (MAPKAP1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SIN1
Synonyms JC310; MIP1; SIN1; SIN1b; SIN1g
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200885 representing NM_024117
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTCTTGGACAATCCAACTATCATTCTAGCTCATATTCGACAGTCACATGTGACCAGTGATGACA
CGGGAATGTGTGAGATGGTTCTCATTGATCATGATGTTGACCTAGAGAAGATTCATCCTCCTTCAATGCC
TGGAGACAGTGGGTCAGAAATTCAGGGAAGCAATGGTGAGACTCAGGGCTATGTATATGCCCAGTCAGTC
GATATTACCTCAAGTTGGGACTTTGGTATTAGAAGACGCTCAAACACAGCTCAAAGATTAGAACGACTCC
GAAAAGAGAGACAAAACCAGATCAAATGCAAAAATATTCAGTGGAAAGAAAGAAATTCTAAGCAATCAGC
CCAGGAGTTAAAGTCACTGTTTGAAAAAAAATCTCTCAAAGAGAAGCCTCCAATTTCTGGGAAGCAGTCG
ATATTATCTGTACGCCTAGAACAGTGCCCTCTGCAGCTGAATAACCCTTTTAACGAGTATTCCAAATTTG
ATGGCAAGGGTCATGTAGGTACAACAGCAACCAAGAAGATCGATGTCTACCTCCCTCTGCACTCGAGCCA
GGACAGACTGCTGCCAATGACCGTGGTGACAATGGCCAGCGCCAGGGTGCAGGACCTGATCGGGCTCATC
TGCTGGCAGTATACAAGCGAAGGACGGGAGCCGAAGCTCAATGACAATGTCAGTGCCTACTGCCTGCATA
TTGCTGAGGATGATGGGGAGGTGGACACCGATTTCCCCCCGCTGGATTCCAATGAGCCCATTCATAAGTT
TGGCTTCAGTACTTTGGCCCTGGTTGAAAAGTACTCATCTCCTGGTCTGACATCCAAAGAGTCACTCTTT
GTTCGAATAAATGCTGCTCATGGATTCTCCCTTATTCAGGTGGACAACACAAAGGTTACCATGAAGGAAA
TCTTACTGAAGGCAGTGAAGCGAAGAAAAGGATCCCAGAAAGTTTCAGGTTCAAGGGCAGACGGGGTTTT
TGAGGAGGATTCGCAAATTGACATAGCCACAGTACAGGATATGCTTAGCAGCCACCATTACAAGTCATTC
AAAGTCAGCATGATCCACAGACTGCGATTCACAACCGACGTACAGCTAGGTATCTCTGGAGACAAAGTAG
AGATAGACCCTGTTACGAATCAGAAAGCCAGCACTAAGTTTTGGATTAAGCAGAAACCCATCTCAATCGA
TTCCGACCTGCTCTGTGCCTGTGACCTTGCTGAAGAGAAAAGCCCCAGTCACGCAATATTTAAACTCACG
TATCTAAGCAATCACGACTATAAACACCTCTACTTTGAATCGGACGCTGCTACCGTCAATGAAATTGTGC
TCAAGGTTAACTACATCCTGGAATCGCGAGCTAGCACTGCCCGGGCTGACTACTTTGCTCAAAAACAAAG
AAAACTGAACAGACGTACGAGCTTCAGCTTCCAGAAGGAGAAGAAATCCGGGCAGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200885 representing NM_024117
Red=Cloning site Green=Tags(s)

MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSV
DITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQS
ILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLI
CWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLF
VRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGSRADGVFEEDSQIDIATVQDMLSSHHYKSF
KVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLT
YLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024117
ORF Size 1458 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024117.3, NP_077022.1
RefSeq Size 3287 bp
RefSeq ORF 1461 bp
Locus ID 79109
UniProt ID Q9BPZ7, A0A024R863
Cytogenetics 9q33.3
Protein Families Druggable Genome
MW 54.8 kDa
Summary This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_024117" in other vectors (4)
SKU Description Size Price
RC200885L3 Lenti-ORF clone of MAPKAP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase associated protein 1 (MAPKAP1), transcript variant 2 10 ug
$757.00
RC200885L4 Lenti-ORF clone of MAPKAP1 (mGFP-tagged)-Human mitogen-activated protein kinase associated protein 1 (MAPKAP1), transcript variant 2 10 ug
$757.00
RG200885 MAPKAP1 (tGFP-tagged) - Human mitogen-activated protein kinase associated protein 1 (MAPKAP1), transcript variant 2 10 ug
$489.00 $657.00
SC128200 MAPKAP1 (untagged)-Human mitogen-activated protein kinase associated protein 1 (MAPKAP1), transcript variant 2 10 ug
$457.00

Plasmid Map for RC200885

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200885
Circular map for RC200885

file-alt Citations

Sin1 promotes proliferation and invasion of prostate Cancer cells by modulating mTORC2-AKT and AR signaling cascades
Huang, Feng, Cai et al
Life Sci. (2020) 248, 117449 DOI: 10.1016/j.lfs.2020.117449
Cited by 12
AI Summary: In this study, the pCMV6-Entry-Sin1-DDK-Myc (PCMV-Sin1) plasmid from OriGene was used to investigate the role of Sin1 in prostate cancer cell proliferation and invasion by enabling Sin1 overexpression experiments.
Snippet: …The MAPKAP1/Sin1 and control (scrambled) siRNAs were purchased from Santa Cruz (Dallas, TX, USA). The pCMV6-Entry-Sin1-DDK-Myc (PCMV-Sin1) plasmid was from Origene (Beijing, China). The pcDNA3.1-AR-His plasmid and pGMAR-Lu firefly luciferase reporter vector containing human Sin1-promoter were constructed
Showing 1-1 of 1 papers.
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