OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

MRPL4 (NM_146388) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC200861
MRPL4 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA315442 Rabbit polyclonal anti-MRPL4 antibody
100 ul
$380.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "MRPL4 or NM_146388"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MRPL4
Synonyms CGI-28; L4mt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200861 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCAGTTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCCC
TGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTCCCGGAGCCCGT
GCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAGGCCTGGGTCGAGTCCTTGCGG
GGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCACCCCGATGTTTTCGCCACCGCGCCCAGGCTGG
ACATACTGCACCAGGTTGCTATGTGGCAGAAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAG
AGCCGAGGTGCGGGGCGGTGGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGC
ATCCGCTCTCCGCTCTGGCGAGGAGGAGGTGTTGCCCATGGCCCCCGGGGCCCCACAAGTTACTACTACA
TGCTGCCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGACCGTCAAGCTGGCCCAGGACGACCT
GCACATCATGGACTCCCTAGAGCTGCCCACCGGAGACCCACAGTACCTGACAGAGCTGGCGCACTACCGC
CGCTGGGGGGACTCCGTACTCCTCGTGGACTTAACACACGAGGAGATGCCACAGAGCATCGTGGAGGCCA
CCTCTAGGCTTAAGACCTTCAACTTGATCCCGGCTGTTGGTGAGCAAAGAGCCCAGGCCCCTAGAGTGCG
CATGTGCAGGCTCCGCTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200861 protein sequence
Red=Cloning site Green=Tags(s)

MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLR
GFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGS
IRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYR
RWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGEQRAQAPRVRMCRLRC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146388
ORF Size 789 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146388.2
RefSeq Size 2404 bp
RefSeq ORF 792 bp
Locus ID 51073
UniProt ID Q9BYD3
Cytogenetics 19p13.2
Domains Ribosomal_L4
MW 29.5 kDa
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. provided by RefSeq, Jul 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_146388" in other vectors (5)
SKU Description Size Price
RC200861L3 Lenti ORF clone of Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC200861L4 Lenti ORF clone of Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged 10 ug
$600.00
RG200861 MRPL4 (tGFP-tagged) - Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$489.00 $500.00
SC101186 MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$300.00
SC320600 MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$300.00

Plasmid Map for RC200861

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200861
Circular map for RC200861

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products