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MRPL4 (NM_146388) Human Untagged Clone

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SKU
SC101186
MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3
  $300.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRPL4
Synonyms CGI-28; L4mt
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_146388, the custom clone sequence may differ by one or more nucleotides


ATGCTGCAGTTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCCC
TGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTCCCGGAGCCCGT
GCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAGGCCTGGGTCGAGTCCTTGCGG
GGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCACCCCGATGTTTTCGCCACCGCGCCCAGGCTGG
ACATACTGCACCAGGTTGCTATGTGGCAGAAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAG
AGCCGAGGTGCGGGGCGGTGGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGC
ATCCGCTCTCCGCTCTGGCGAGGAGGAGGTGTTGCCCATGGCCCCCGGGGCCCCACAAGTTACTACTACA
TGCTGCCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGACCGTCAAGCTGGCCCAGGACGACCT
GCACATCATGGACTCCCTAGAGCTGCCCACCGGAGACCCACAGTACCTGACAGAGCTGGCGCACTACCGC
CGCTGGGGGGACTCCGTACTCCTCGTGGACTTAACACACGAGGAGATGCCACAGAGCATCGTGGAGGCCA
CCTCTAGGCTTAAGACCTTCAACTTGATCCCGGCTGTTGGTGAGCAAAGAGCCCAGGCCCCTAGAGTGCG
CATGTGCAGGCTCCGCTGTTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_146388 unedited
NNCCTGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGCCAAAG
TAGCTAGGCCTACGGAAGCTGGGTCTTCTTGCTGTGAGGTCGCGTTCCCCAGTGTTACGG
AGGGTCCTTGAGGCAGGAGTGAAAATTGGGTCTGGGGGTTAGTCCTGGGGTGGAGGTCTG
GGCACGCCGGGTCGGACCCCCTCCATCTTCGGTTTTGCACACCCCGCTTTCCAGCGCGGA
GTCGCGGCGGGTAGGGCGGCGTCGCGTGCGTGACGTCATCCAGCGGCGCCTCGCGAGGCT
CCAGTGGCCTTGACCTCCCGCGGCGTGGGAGGCTGCGCGGCGATGCTGCAGTTCGTCCGG
GCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCCCTGGCGGAA
GAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTCCCGGAGCCC
GTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAGGCCTGGGTC
GAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCACCCCGATGTT
TTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGTTGCTATGTGGCAGAAGAACTTC
AAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGAGGTGCGGNNGCGGTGGCCGAAA
GCCTTGGCCGCAGAAAGCACTGGGCGGGCCCGGCATGGCAGCATCCGCTCTTCGCTCTGG
CGAGGANGNANGTGTTGCCCATGGGGCCCCCGGGCCCCACAAGTTACTACTACATGCTGC
CNNATGAAGTGCGGNCGCTGGNTCTCAAAGTGCACTGACCGTCAAGCTGGCCAGGACGAC
TGCCATCATGGACTCCCTA
Restriction Sites NotI-NotI |
ACCN NM_146388
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146388.1, NP_666500.1
RefSeq Size 2404 bp
RefSeq ORF 792 bp
Locus ID 51073
UniProt ID Q9BYD3
Cytogenetics 19p13.2
Domains Ribosomal_L4
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. provided by RefSeq, Jul 2008
Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes an isoform (b) which has a shorter and distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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"NM_146388" in other vectors (5)
SKU Description Size Price
RC200861 MRPL4 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$300.00
RC200861L3 Lenti ORF clone of Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC200861L4 Lenti ORF clone of Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged 10 ug
$600.00
RG200861 MRPL4 (tGFP-tagged) - Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$489.00 $500.00
SC320600 MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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