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LRFN3 (NM_024509) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200850
LRFN3 (Myc-DDK-tagged)-Human leucine rich repeat and fibronectin type III domain containing 3 (LRFN3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $585.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRFN3
Synonyms FIGLER1; SALM4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200850 representing NM_024509
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATCCTCCCGTTGCTCCTGTGCCTGCTGCCGCTGGCCCCTGCCTCATCCCCACCCCAGTCAGCCA
CACCCAGCCCATGTCCCCGCCGCTGCCGCTGCCAGACACAGTCGCTGCCCCTAAGCGTGCTGTGCCCAGG
GGCAGGCCTCCTGTTCGTGCCACCCTCGCTGGACCGCCGGGCAGCCGAGCTGCGGCTGGCAGACAACTTC
ATCGCCTCCGTGCGCCGCCGCGACCTGGCCAACATGACAGGCCTGCTGCATCTGAGCCTGTCGCGGAACA
CCATCCGCCACGTGGCTGCCGGCGCCTTCGCCGACCTGCGGGCCCTGCGTGCCCTGCACCTGGATGGCAA
CCGGCTGACCTCACTGGGCGAGGGCCAGCTGCGCGGCCTGGTCAACTTGCGCCACCTCATCCTCAGCAAC
AACCAGCTGGCAGCGCTGGCGGCCGGCGCCCTGGATGATTGTGCCGAGACACTGGAGGACCTCGACCTCT
CCTACAACAACCTCGAGCAGCTGCCCTGGGAGGCCCTGGGCCGCCTGGGCAACGTCAACACGTTGGGCCT
CGACCACAACCTGCTGGCTTCTGTGCCCGCCGGCGCTTTTTCCCGCCTGCACAAGCTGGCCCGGCTGGAC
ATGACCTCCAACCGCCTGACCACAATCCCACCCGACCCACTCTTCTCCCGCCTGCCCCTGCTCGCCAGGC
CCCGGGGCTCGCCCGCCTCTGCCCTGGTGCTGGCCTTTGGCGGGAACCCCCTGCACTGCAACTGCGAGCT
GGTGTGGCTGCGTCGCCTGGCGCGGGAGGACGACCTCGAGGCCTGCGCGTCCCCACCTGCTCTGGGCGGC
CGCTACTTCTGGGCGGTGGGCGAGGAGGAGTTTGTCTGCGAGCCGCCCGTGGTGACTCACCGCTCACCAC
CTCTGGCTGTGCCCGCAGGTCGGCCGGCTGCCCTGCGCTGCCGGGCAGTGGGGGACCCAGAGCCCCGTGT
GCGTTGGGTGTCACCCCAGGGCCGGCTGCTAGGCAACTCAAGCCGTGCCCGCGCCTTCCCCAATGGGACG
CTGGAGCTGCTGGTCACCGAGCCGGGTGATGGTGGCATCTTCACCTGCATTGCGGCCAATGCAGCTGGCG
AGGCCACAGCTGCTGTGGAGCTGACTGTGGGTCCCCCACCACCTCCTCAGCTAGCCAACAGCACCAGCTG
TGACCCCCCGCGGGACGGGGATCCTGATGCTCTCACCCCACCCTCCGCTGCCTCTGCTTCTGCCAAGGTG
GCCGACACTGGGCCCCCTACCGACCGTGGCGTCCAGGTGACTGAGCACGGGGCCACAGCTGCTCTTGTCC
AGTGGCCGGATCAGCGGCCTATCCCGGGCATCCGCATGTACCAGATCCAGTACAACAGCTCGGCTGATGA
CATCCTCGTCTACAGGATGATCCCGGCGGAGAGCCGCTCGTTCCTGCTGACGGACCTGGCGTCAGGCCGG
ACCTACGATCTGTGCGTGCTCGCCGTGTATGAGGACAGCGCCACGGGGCTCACGGCCACGCGGCCTGTGG
GCTGCGCCCGCTTCTCCACCGAACCTGCGCTGCGGCCATGCGGGGCGCCGCACGCTCCCTTCCTGGGCGG
CACGATGATCATCGCGCTGGGCGGCGTCATCGTAGCCTCGGTACTGGTCTTCATCTTCGTGCTGCTAATG
CGCTACAAGGTGCACGGCGGCCAGCCCCCCGGCAAGGCCAAGATTCCCGCGCCTGTTAGCAGCGTTTGCT
CCCAGACCAACGGCGCCCTGGGCCCCACGCCCACGCCCGCCCCGCCCGCCCCGGAGCCCGCGGCGCTCAG
GGCCCACACCGTGGTCCAGCTGGACTGCGAGCCCTGGGGGCCCGGCCACGAACCTGTGGGACCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200850 representing NM_024509
Red=Cloning site Green=Tags(s)

MAILPLLLCLLPLAPASSPPQSATPSPCPRRCRCQTQSLPLSVLCPGAGLLFVPPSLDRRAAELRLADNF
IASVRRRDLANMTGLLHLSLSRNTIRHVAAGAFADLRALRALHLDGNRLTSLGEGQLRGLVNLRHLILSN
NQLAALAAGALDDCAETLEDLDLSYNNLEQLPWEALGRLGNVNTLGLDHNLLASVPAGAFSRLHKLARLD
MTSNRLTTIPPDPLFSRLPLLARPRGSPASALVLAFGGNPLHCNCELVWLRRLAREDDLEACASPPALGG
RYFWAVGEEEFVCEPPVVTHRSPPLAVPAGRPAALRCRAVGDPEPRVRWVSPQGRLLGNSSRARAFPNGT
LELLVTEPGDGGIFTCIAANAAGEATAAVELTVGPPPPPQLANSTSCDPPRDGDPDALTPPSAASASAKV
ADTGPPTDRGVQVTEHGATAALVQWPDQRPIPGIRMYQIQYNSSADDILVYRMIPAESRSFLLTDLASGR
TYDLCVLAVYEDSATGLTATRPVGCARFSTEPALRPCGAPHAPFLGGTMIIALGGVIVASVLVFIFVLLM
RYKVHGGQPPGKAKIPAPVSSVCSQTNGALGPTPTPAPPAPEPAALRAHTVVQLDCEPWGPGHEPVGP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024509
ORF Size 1884 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024509.2
RefSeq Size 2549 bp
RefSeq ORF 1887 bp
Locus ID 79414
UniProt ID Q9BTN0
Cytogenetics 19q13.12
Domains FN3, ig, LRR, LRRCT
Protein Families Transmembrane
MW 66.3 kDa
Summary Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Promotes neurite outgrowth in hippocampal neurons (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024509" in other vectors (4)
SKU Description Size Price
RC200850L3 Lenti-ORF clone of LRFN3 (Myc-DDK-tagged)-Human leucine rich repeat and fibronectin type III domain containing 3 (LRFN3) 10 ug
$885.00
RC200850L4 Lenti-ORF clone of LRFN3 (mGFP-tagged)-Human leucine rich repeat and fibronectin type III domain containing 3 (LRFN3) 10 ug
$885.00
RG200850 LRFN3 (tGFP-tagged) - Human leucine rich repeat and fibronectin type III domain containing 3 (LRFN3) 10 ug
$785.00
SC111418 LRFN3 (untagged)-Human leucine rich repeat and fibronectin type III domain containing 3 (LRFN3) 10 ug
$585.00

Plasmid Map for RC200850

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200850
Circular map for RC200850

file-alt Citations

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