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PCDHB5 (NM_015669) Human Tagged ORF Clone

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SKU
RC200848
PCDHB5 (Myc-DDK-tagged)-Human protocadherin beta 5 (PCDHB5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $728.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCDHB5
Synonyms PCDH-BETA5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200848 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACTGCGCTAGCAAAAACGCCACAGAAAAGGCAAGTTATGTTTCTTGCTATATTGTTGCTTTTGT
GGGAGGCTGGCTCTGAGGCAGTTAGGTATTCCATACCAGAAGAAACAGAAAGTGGCTATTCTGTGGCCAA
CCTGGCAAAAGACCTGGGTCTTGGGGTGGGGGAACTGGCCACTCGGGGCGCGCGAATGCATTACAAAGGA
AACAAAGAGCTCTTGCAGCTTGATATAAAGACCGGCAATTTGCTTCTATATGAAAAACTAGACCGGGAGG
TGATGTGCGGGGCGACAGAACCCTGTATATTGCATTTCCAGCTCTTACTAGAAAATCCAGTGCAGTTTTT
TCAAACTGATCTGCAGCTCACAGATATAAATGACCATGCCCCAGAGTTCCCAGAGAAGGAAATGCTCCTA
AAAATCCCAGAGAGCACCCAGCCAGGGACTGTGTTTCCCTTAAAAATAGCCCAGGACTTTGACATAGGTA
GCAACACTGTTCAGAACTACACAATCAGCCCAAATTCACACTTTCATGTTGCTACGCATAATCGCGGAGA
TGGCAGAAAATACCCAGAGCTGGTGCTGGACAAAGCGCTGGACCGGGAGGAGCGGCCTGAGCTCAGCTTA
ACACTCACTGCACTGGACGGTGGGGCTCCGCCCAGGTCCGGGACCACCACAATTCGCATTGTCGTCTTGG
ATAATAATGACAACGCCCCCGAATTTTTACAATCATTCTATGAGGTACAGGTGCCCGAGAACAGCCCCCT
TAACTCCTTAGTTGTCGTTGTCTCCGCTCGAGATTTAGATGCAGGAGCATATGGGAGTGTAGCCTATGCT
CTATTCCAAGGCGATGAAGTTACTCAACCATTTGTAATAGACGAGAAAACAGCAGAAATTCGCCTGAAAA
GGGCATTGGATTTCGAGGCAACTCCATATTATAACGTGGAAATTGTAGCCACAGATGGTGGGGGCCTTTC
AGGAAAATGCACTGTGGCTATAGAAGTGGTGGATGTGAATGACAACGCCCCTGAACTCACCATGTCTACG
CTCTCCAGCCCTACCCCAGAAAATGCCCCGGAAACTGTAGTTGCCGTTTTCAGTGTTTCTGATCCAGACT
CCGGGGACAACGGTAGGATGATTTGCTCCATCCAGAATGATCTCCCCTTTCTTTTGAAGCCCACATTAAA
AAACTTTTACACCCTAGTGACACAGAGAACACTGGACAGAGAGAGCCAAGCCGAGTACAACATCACCATC
ACTGTCACCGACATGGGGACACCCAGGCTGAAAACCGAGCACAACATAACGGTCCTGGTCTCCGACGTCA
ATGACAACGCCCCCGCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGAGAGAACAACAGCCCCGCCCT
GCACATCGGCAGTGTCAGCGCCACAGACAGAGACTCAGGCACCAACGCCCAGGTCACCTACTCGCTGCTG
CCGCCCCAGAATCCACACCTGCGCCTCGCCTCCCTGGTCTCCATCAACGCGGACAACGGCCACCTGTTTG
CCCTCAGGTCGCTGGACTACGAGGCCCTGCAGGCGTTCGAGTTCCGCGTGGGAGCCACAGACCGCGGCTC
CCCGGCGCTGAGCAGCGAGGCGCTGGTGCGCGTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTCGTG
CTGTATCCGCTGCAGAACGGCTCGGCGCCTTGCACCGAGCTGGTGCCCCGGGCGGCCGAGCCGGGCTACC
TGGTGACCAAGGTGGTGGCGGTGGACGGTGACTCGGGCCAGAACGCCTGGCTGTCGTACCAGCTGCTCAA
GGCCACGGAGCCCGGGCTGTTCAGCATGTGGGCGCACAATGGCGAGGTGCGCACCGCCAGGCTGCTGAGC
GAGCGCGACGCGGCCAAGCACAGGCTGGTGGTGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGGCCA
CCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCCGCTGCCGGAGGCGGCCCC
GGCCCAGGCCCAGGCCGACTCGCTCACTGTCTACCTGGTGGTGGCATTGGCCTCGGTGTCGTCGCTCTTC
CTCTTTTCGGTGCTCCTGTTCGTGGCAGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCCCGGTCGGTCGCT
GCTCGGTGCCCGAGGGCCCCTTTCCAGGGCATCTGGTGGACGTGAGCGGCACCGGGACCCTATCCCAGAG
CTACCACTACGAGGTGTGTTTGACCGGAGACTCAGGGGCCGGCGAGTTCAAGTTCCTGAAGCCGATTATT
CCTAACCTTTTGCCCCAGGGCGCTGGTGAAGAAATAGGGAAAACTGCTGCCTTCCGGAATAGCTTTGGAT
TAAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200848 protein sequence
Red=Cloning site Green=Tags(s)

METALAKTPQKRQVMFLAILLLLWEAGSEAVRYSIPEETESGYSVANLAKDLGLGVGELATRGARMHYKG
NKELLQLDIKTGNLLLYEKLDREVMCGATEPCILHFQLLLENPVQFFQTDLQLTDINDHAPEFPEKEMLL
KIPESTQPGTVFPLKIAQDFDIGSNTVQNYTISPNSHFHVATHNRGDGRKYPELVLDKALDREERPELSL
TLTALDGGAPPRSGTTTIRIVVLDNNDNAPEFLQSFYEVQVPENSPLNSLVVVVSARDLDAGAYGSVAYA
LFQGDEVTQPFVIDEKTAEIRLKRALDFEATPYYNVEIVATDGGGLSGKCTVAIEVVDVNDNAPELTMST
LSSPTPENAPETVVAVFSVSDPDSGDNGRMICSIQNDLPFLLKPTLKNFYTLVTQRTLDRESQAEYNITI
TVTDMGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLL
PPQNPHLRLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSSEALVRVLVLDANDNSPFV
LYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFSMWAHNGEVRTARLLS
ERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSLF
LFSVLLFVAVRLCRRSRAAPVGRCSVPEGPFPGHLVDVSGTGTLSQSYHYEVCLTGDSGAGEFKFLKPII
PNLLPQGAGEEIGKTAAFRNSFGLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015669
ORF Size 2385 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015669.5
RefSeq Size 2926 bp
RefSeq ORF 2388 bp
Locus ID 26167
UniProt ID Q9Y5E4, Q59FR6
Cytogenetics 5q31.3
Domains CA
Protein Families Druggable Genome, Transmembrane
MW 86.4 kDa
Summary This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. provided by RefSeq, Jul 2008
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"NM_015669" in other vectors (4)
SKU Description Size Price
RC200848L3 Lenti ORF clone of Human protocadherin beta 5 (PCDHB5), Myc-DDK-tagged 10 ug
$1,028.00
RC200848L4 Lenti ORF clone of Human protocadherin beta 5 (PCDHB5), mGFP tagged 10 ug
$1,028.00
RG200848 PCDHB5 (tGFP-tagged) - Human protocadherin beta 5 (PCDHB5) 10 ug
$789.00 $928.00
SC114606 PCDHB5 (untagged)-Human protocadherin beta 5 (PCDHB5) 10 ug
$729.00

Plasmid Map for RC200848

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200848
Circular map for RC200848

file-alt Citations

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