METRN (NM_024042) Human Tagged ORF Clone

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SKU
RC200830
METRN (Myc-DDK-tagged)-Human meteorin, glial cell differentiation regulator (METRN)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol METRN
Synonyms C16orf23; c380A1.2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200830 representing NM_024042
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTTCCCGGCCGCGGCGCTGCTCTGCGCGCTGTGCTGCGGCCTCCTGGCCCCGGCTGCCCGCGCCG
GCTACTCCGAGGAGCGCTGCAGCTGGAGGGGCAGCGGCCTCACCCAGGAGCCCGGCAGCGTGGGGCAGCT
GGCCCTGGCCTGTGCGGAGGGCGCGGTTGAGTGGCTGTACCCGGCTGGGGCGCTGCGCCTGACCCTGGGC
GGCCCCGATCCCAGAGCGCGGCCCGGCATCGCCTGTCTGCGGCCGGTGCGGCCCTTCGCGGGCGCCCAGG
TCTTCGCGGAGCGCGCAGGGGGCGCCCTGGAGCTGCTGCTGGCCGAGGGCCCGGGCCCGGCAGGGGGCCG
CTGCGTGCGCTGGGGTCCCCGCGAGCGCCGGGCCCTCTTCCTGCAGGCCACGCCGCACCAGGACATCAGC
CGCCGCGTGGCCGCCTTCCGCTTTGAGCTGCGCGAGGACGGGCGCCCCGAGCTGCCCCCGCAGGCCCACG
GTCTCGGCGTAGACGGTGCCTGCAGGCCCTGCAGCGACGCTGAGCTGCTCCTGGCCGCATGCACCAGCGA
CTTCGTAATTCACGGGATCATCCATGGGGTCACCCATGACGTGGAGCTGCAGGAGTCTGTCATCACTGTG
GTGGCCGCCCGTGTCCTCCGCCAGACACCGCCGCTGTTCCAGGCGGGGCGATCCGGGGACCAGGGGCTGA
CCTCCATTCGTACCCCACTGCGCTGTGGCGTCCACCCGGGCCCAGGCACCTTCCTCTTCATGGGCTGGAG
CCGCTTTGGGGAGGCCCGGCTGGGCTGTGCCCCACGATTCCAGGAGTTCCGCCGTGCCTACGAGGCTGCC
CGTGCTGCCCACCTCCACCCCTGCGAGGTGGCGCTGCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200830 representing NM_024042
Red=Cloning site Green=Tags(s)

MGFPAAALLCALCCGLLAPAARAGYSEERCSWRGSGLTQEPGSVGQLALACAEGAVEWLYPAGALRLTLG
GPDPRARPGIACLRPVRPFAGAQVFAERAGGALELLLAEGPGPAGGRCVRWGPRERRALFLQATPHQDIS
RRVAAFRFELREDGRPELPPQAHGLGVDGACRPCSDAELLLAACTSDFVIHGIIHGVTHDVELQESVITV
VAARVLRQTPPLFQAGRSGDQGLTSIRTPLRCGVHPGPGTFLFMGWSRFGEARLGCAPRFQEFRRAYEAA
RAAHLHPCEVALH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024042
ORF Size 879 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024042.4
RefSeq Size 1109 bp
RefSeq ORF 882 bp
Locus ID 79006
UniProt ID Q9UJH8
Cytogenetics 16p13.3
Protein Families Secreted Protein
MW 31 kDa
Summary Meteorin regulates glial cell differentiation and promotes the formation of axonal networks during neurogenesis (Nishino et al., 2004 PubMed 15085178).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_024042" in other vectors (4)
SKU Description Size Price
RC200830L3 Lenti ORF clone of Human meteorin, glial cell differentiation regulator (METRN), Myc-DDK-tagged 10 ug
$600.00
RC200830L4 Lenti ORF clone of Human meteorin, glial cell differentiation regulator (METRN), mGFP tagged 10 ug
$600.00
RG200830 METRN (tGFP-tagged) - Human meteorin, glial cell differentiation regulator (METRN) 10 ug
$500.00
SC112295 METRN (untagged)-Human meteorin, glial cell differentiation regulator (METRN) 10 ug
$300.00

Plasmid Map for RC200830

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Cloning scheme for RC200830

Circular map for RC200830

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