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SPSB2 (NM_032641) Human Tagged ORF Clone

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SKU
RC200801
SPSB2 (Myc-DDK-tagged)-Human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "SPSB2 or NM_032641"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPSB2
Synonyms GRCC9; SSB2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200801 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCAGACAGCTCTGGCAGGGGGCAGCAGCAGCACCCCCACGCCACAGGCCCTGTACCCTGACCTCT
CCTGTCCCGAGGGCTTGGAAGAGCTGCTGTCTGCACCCCCTCCTGACCTGGGGGCCCAGCGGCGCCACGG
TTGGAACCCCAAAGACTGTTCAGAGAACATCGAGGTCAAGGAAGGAGGGTTGTACTTTGAGCGGCGGCCC
GTGGCCCAGAGCACTGATGGGGCCCGGGGTAAGAGGGGCTATTCAAGGGGCCTGCACGCCTGGGAGATCA
GCTGGCCCCTAGAGCAGAGGGGCACGCATGCCGTGGTGGGCGTGGCCACGGCCCTCGCCCCGCTGCAGAC
TGACCACTACGCGGCGCTGCTGGGCAGCAACAGCGAGTCGTGGGGCTGGGACATCGGGCGGGGGAAGCTG
TACCATCAGAGCAAGGGGCCCGGAGCCCCCCAGTATCCAGCGGGAACTCAGGGTGAGCAGCTGGAGGTGC
CAGAGAGACTGCTGGTGGTTCTGGACATGGAGGAGGGAACTCTGGGCTACGCTATTGGGGGCACCTACCT
GGGGCCAGCATTCCGCGGACTGAAGGGCAGGACCCTCTATCCGGCAGTAAGCGCTGTCTGGGGCCAGTGC
CAGGTCCGCATCCGCTACCTGGGCGAAAGGAGAGCGGAGCCACACTCCCTTCTGCACCTGAGCCGCCTGT
GTGTGCGCCACAACCTGGGGGATACCCGGCTCGGCCAGGTGTCTGCCCTGCCCTTGCCCCCTGCCATGAA
GCGCTACCTGCTCTACCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200801 protein sequence
Red=Cloning site Green=Tags(s)

MGQTALAGGSSSTPTPQALYPDLSCPEGLEELLSAPPPDLGAQRRHGWNPKDCSENIEVKEGGLYFERRP
VAQSTDGARGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQTDHYAALLGSNSESWGWDIGRGKL
YHQSKGPGAPQYPAGTQGEQLEVPERLLVVLDMEEGTLGYAIGGTYLGPAFRGLKGRTLYPAVSAVWGQC
QVRIRYLGERRAEPHSLLHLSRLCVRHNLGDTRLGQVSALPLPPAMKRYLLYQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032641
ORF Size 789 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032641.4
RefSeq Size 1272 bp
RefSeq ORF 792 bp
Locus ID 84727
UniProt ID Q99619
Cytogenetics 12p13.31
Domains SOCS, SPRY
Protein Families Druggable Genome
MW 28.6 kDa
Summary This gene encodes a member of a subfamily of proteins containing a central SPRY (repeats in splA and RyR) domain and a C-terminal suppressor of cytokine signaling (SOCS) box. This protein plays a role in cell signaling. This gene is present in a gene-rich cluster on chromosome 12p13 in the vicinity of the CD4 antigen and triosephosphate isomerase genes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2016
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"NM_032641" in other vectors (6)
SKU Description Size Price
RC200801L1 Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200801L2 Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1, mGFP tagged 10 ug
$600.00
RC200801L3 Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200801L4 Lenti ORF clone of Human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG200801 SPSB2 (tGFP-tagged) - Human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1 10 ug
$489.00 $500.00
SC319450 SPSB2 (untagged)-Human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1 10 ug
$300.00

Plasmid Map for RC200801

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200801
Circular map for RC200801

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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