DDX19A (NM_018332) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC200751
DDX19A (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19A (DDX19A)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DDX19A
Synonyms DDX19-DDX19L; DDX19L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200751 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCGACTCGTGGGCCCTGGCGGTGGACGAGCAGGAAGCGGCTGTCAAGTCGATGACCAATTTGC
AGATCAAGGAAGAGAAAGTCAAAGCAGATACCAATGGTATTATCAAAACCAGTACCACTGCCGAGAAAAC
AGATGAAGAGGAGAAAGAGGACAGAGCTGCCCAGTCCTTACTCAACAAGCTGATCAGAAGCAACCTTGTT
GATAACACAAACCAAGTGGAAGTCCTGCAACGGGATCCAAACTCCCCTCTGTACTCGGTGAAGTCGTTTG
AAGAGCTTCGGCTGAAACCACAGCTTCTCCAGGGAGTCTATGCCATGGGCTTCAATCGACCCTCCAAGAT
ACAAGAGAACGCATTACCCATGATGCTTGCTGAACCCCCACAGAATCTGATTGCCCAGTCTCAGTCTGGC
ACTGGTAAAACAGCTGCCTTTGTCTTAGCCATGCTCAGCCGAGTGGAGCCATCAGACAGATACCCCCAGT
GTCTGTGCCTCTCCCCAACATATGAGCTGGCGCTTCAAACAGGAAAAGTGATTGAGCAGATGGGCAAATT
TTACCCAGAACTGAAGCTTGCCTATGCCGTTCGAGGCAATAAATTGGAAAGAGGCCAGAAGATCAGTGAG
CAGATTGTCATTGGCACCCCTGGGACCGTGCTGGACTGGTGCTCCAAGCTCAAGTTCATTGATCCCAAGA
AAATCAAGGTGTTTGTTCTGGATGAGGCTGATGTCATGATAGCCACTCAGGGCCACCAAGATCAGAGCAT
CCGCATCCAGAGGATGCTGCCCAGGAACTGCCAGATGCTGCTTTTCTCCGCCACCTTTGAAGACTCTGTG
TGGAAGTTTGCCCAGAAAGTGGTCCCAGACCCAAATGTTATCAAACTGAAGCGTGAGGAAGAGACCCTGG
ATACCATCAAGCAGTACTATGTCCTGTGCAGCAGCAGAGACGAGAAGTTCCAGGCCTTGTGTAACCTCTA
CGGGGCCATCACCATTGCTCAAGCCATGATCTTCTGCCATACTCGCAAAACAGCTAGTTGGCTGGCAGCA
GAGCTCTCAAAAGAAGGCCACCAGGTGGCTCTGCTGAGTGGGGAGATGATGGTGGAGCAGAGGGCTGCGG
TGATTGAGCGCTTCCGAGAGGGCAAAGAGAAGGTTTTGGTGACCACCAACGTGTGTGCCCGCGGCATTGA
TGTTGAACAAGTGTCTGTCGTCATCAACTTTGATCTTCCCGTGGACAAGGACGGGAATCCTGACAATGAG
ACCTACCTGCACCGGATCGGGCGCACGGGCCGCTTTGGCAAGAGGGGCCTGGCAGTGAACATGGTGGACA
GCAAGCACAGCATGAACATCCTGAACAGAATCCAGGAGCATTTTAATAAGAAGATAGAAAGATTGGACAC
AGATGATTTGGACGAGATTGAGAAAATAGCCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200751 protein sequence
Red=Cloning site Green=Tags(s)

MATDSWALAVDEQEAAVKSMTNLQIKEEKVKADTNGIIKTSTTAEKTDEEEKEDRAAQSLLNKLIRSNLV
DNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQENALPMMLAEPPQNLIAQSQSG
TGKTAAFVLAMLSRVEPSDRYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISE
QIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSV
WKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAA
ELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNE
TYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018332
ORF Size 1434 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018332.5
RefSeq Size 2918 bp
RefSeq ORF 1437 bp
Locus ID 55308
UniProt ID Q9NUU7
Cytogenetics 16q22.1
Domains DEAD, helicase_C
MW 54 kDa
Summary ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19 functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (By similarity).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_018332" in other vectors (5)
SKU Description Size Price
RC200751L3 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-As) box polypeptide 19A (DDX19A), Myc-DDK-tagged 10 ug
$757.00
RC200751L4 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-As) box polypeptide 19A (DDX19A), mGFP tagged 10 ug
$757.00
RG200751 DDX19A (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-As) box polypeptide 19A (DDX19A) 10 ug
$489.00 $657.00
SC113550 DDX19A (untagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19A (DDX19A) 10 ug
$457.00
SC320851 DDX19A (untagged)-Human DEAD (Asp-Glu-Ala-As) box polypeptide 19A (DDX19A) 10 ug
$457.00

Plasmid Map for RC200751

Download sequence for

Cloning scheme for RC200751

Circular map for RC200751

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products