MCG10 (PCBP4) (NM_033008) Human Tagged ORF Clone

SKU RC200749

PCBP4 (Myc-DDK-tagged)-Human poly(rC) binding protein 4 (PCBP4), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MCG10
• Synonyms: CBP; LIP4; MCG10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200749 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGA; TGCACGGGAAGGAAGTGGGCAGCATCATCGGGAAGAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGAG; CAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCTACAGCA; GCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATG; GTGGAAATGTCTCCAGGCCTCCAGTGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGAT; TGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGACTACGGGTGCCCAGGTACAGGTGGCAGGGGAC; CTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGCCTGATGCCATCATCCTGTGTGTGC; GCCAGATCTGCGCTGTTATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTC; CCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCA; GCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAG; GACTGGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTGTGAT; CGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAACCAA; GCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGTACC; TCATCACTGCCTGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCT; GCCTGCCCCCTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTAT; GCCATCTCCCTCTCCAACTTCATCGGCCTCAAGCCCATGCCCTTCTTGGCTTTACCACCTGCTTCCCCAG; GGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGCG; GCAGAAATTCTCCCCCTAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200749 protein sequence; Red=Cloning site Green=Tags(s); ; MSGSDGGLEEEPELSITLTLRMLMHGKEVGSIIGKKGETVKRIREQSSARITISEGSCPERITTITGSTA; AVFHAVSMIAFKLDEDLCAAPANGGNVSRPPVTLRLVIPASQCGSLIGKAGTKIKEIRETTGAQVQVAGD; LLPNSTERAVTVSGVPDAIILCVRQICAVILESPPKGATIPYHPSLSLGTVLLSANQGFSVQGQYGAVTP; AEVTKLQQLSSHAVPFATPSVVPGLDPGTQTSSQEFLVPNDLIGCVIGRQGSKISEIRQMSGAHIKIGNQ; AEGAGERHVTITGSPVSIALAQYLITACLETAKSTSGGTPSSAPADLPAPFSPPLTALPTAPPGLLGTPY; AISLSNFIGLKPMPFLALPPASPGPPPGLAAYTAKMAAANGSKKAERQKFSPY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033008
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033008.3
• RefSeq Size: 2040 bp
• RefSeq ORF: 1212 bp
• Locus ID: 57060
• UniProt ID: P57723
• Cytogenetics: 3p21.2
• Domains: KH
• MW: 41.5 kDa
• Summary: This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G(2)-M. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described, but the full-length nature for only some has been determined. [provided by RefSeq, Jul 2008]