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ESS2 (NM_022719) Human Tagged ORF Clone

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SKU
RC200743
DGCR14 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 14 (DGCR14)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ESS2
Synonyms bis1; DGCR13; DGCR14; DGS-H; DGS-I; DGSH; DGSI; ES2; Es2el; ESS-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200743 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACGCCGGGCGCATCAGCGTCGTCCTTGTTGCTTCCCGCCGCGTCCAGGCCCCCGAGGAAGCGCG
AGGCGGGAGAGGCTGGGGCTGCGACGAGCAAGCAGCGGGTCCTGGACGAGGAAGAGTATATCGAGGGCCT
CCAGACGGTCATCCAAAGGGATTTCTTTCCTGATGTGGAGAAGCTCCAGGCACAGAAGGAGTACCTGGAA
GCCGAGGAGAATGGAGACTTGGAACGGATGCGCCAGATTGCCATCAAGTTTGGCTCTGCCTTGGGCAAGA
TGTCCCGGGAGCCCCCGCCACCCTATGTGACTCCAGCCACATTTGAAACCCCTGAGGTGCATGCAGGCAC
TGGAGTGGTGGGCAACAAGCCCAGGCCCCGCGGCCGAGGCCTGGAGGATGGAGAGGCTGGAGAGGAGGAG
GAGAAGGAGCCGCTGCCCAGCCTAGATGTCTTCCTGAGCCGCTACACGAGTGAGGACAATGCCTCCTTCC
AGGAGATCATGGAGGTGGCCAAGGAGAGAAGCCGGGCACGCCACGCTTGGCTCTACCAGGCTGAGGAAGA
GTTTGAGAAGAGGCAGAAAGATAATCTCGAACTCCCGTCAGCAGAGCACCAGGCCATCGAGAGCAGCCAG
GCCAGTGTGGAGACCTGGAAGTACAAGGCCAAGAATTCCCTCATGTACTATCCAGAGGGTGTCCCTGACG
AGGAGCAGCTGTTTAAGAAGCCCCGGCAGGTGGTACATAAGAACACGCGCTTCCTTAGGGACCCCTTCAG
CCAAGCCCTGAGCAGGTGCCAGCTCCAGCAGGCAGCCGCCCTCAATGCCCAGCACAAACAGGGCAAGGTG
GGCCCCGATGGCAAGGAGCTGATCCCCCAGGAGTCCCCTCGAGTGGGTGGATTTGGATTTGTTGCCACTC
CTTCCCCTGCCCCTGGTGTGAACGAGTCCCCGATGATGACCTGGGGGGAGGTTGAGAACACACCCTTGAG
AGTTGAAGGGTCGGAAACGCCCTACGTGGACAGGACACCCGGCCCAGCTTTTAAGATCCTGGAGCCAGGC
CGCAGGGAGCGGCTGGGTCTGAAGATGGCCAACGAGGCCGCTGCCAAGAACCGGGCCAAGAAGCAGGAAG
CCTTGCGGAGAGTGACGGAGAATCTGGCCAGCCTCACCCCCAAAGGCCTGAGCCCAGCCATGTCGCCAGC
CCTACAGCGCCTTGTGAGCAGGACGGCCAGCAAGTACACAGACCGGGCCCTGCGGGCCAGCTACACACCA
TCCCCAGCACGCTCCACCCACCTCAAGACCCCGGCCAGTGGGCTGCAGACCCCCACAAGCACACCGGCGC
CTGGCTCTGCCACACGCACCCCTCTCACACAGGACCCGGCCTCCATCACGGACAACCTGCTGCAGCTCCC
TGCCCGGCGCAAAGCTTCGGACTTCTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200743 protein sequence
Red=Cloning site Green=Tags(s)

METPGASASSLLLPAASRPPRKREAGEAGAATSKQRVLDEEEYIEGLQTVIQRDFFPDVEKLQAQKEYLE
AEENGDLERMRQIAIKFGSALGKMSREPPPPYVTPATFETPEVHAGTGVVGNKPRPRGRGLEDGEAGEEE
EKEPLPSLDVFLSRYTSEDNASFQEIMEVAKERSRARHAWLYQAEEEFEKRQKDNLELPSAEHQAIESSQ
ASVETWKYKAKNSLMYYPEGVPDEEQLFKKPRQVVHKNTRFLRDPFSQALSRCQLQQAAALNAQHKQGKV
GPDGKELIPQESPRVGGFGFVATPSPAPGVNESPMMTWGEVENTPLRVEGSETPYVDRTPGPAFKILEPG
RRERLGLKMANEAAAKNRAKKQEALRRVTENLASLTPKGLSPAMSPALQRLVSRTASKYTDRALRASYTP
SPARSTHLKTPASGLQTPTSTPAPGSATRTPLTQDPASITDNLLQLPARRKASDFF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022719
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022719.3
RefSeq Size 5405 bp
RefSeq ORF 1431 bp
Locus ID 8220
UniProt ID Q96DF8
Cytogenetics 22q11.2
MW 52.6 kDa
Summary This gene is located within the minimal DGS critical region (MDGCR) thought to contain the gene(s) responsible for a group of developmental disorders. These disorders include DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome, and some familial or sporadic conotruncal cardiac defects which have been associated with microdeletion of 22q11.2. The encoded protein may be a component of C complex spliceosomes, and the orthologous protein in the mouse localizes to the nucleus. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Dec 2015
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Documents
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"NM_022719" in other vectors (6)
SKU Description Size Price
RC200743L1 Lenti ORF clone of Human DiGeorge syndrome critical region gene 14 (DGCR14), Myc-DDK-tagged 10 ug
$757.00
RC200743L2 Lenti ORF clone of Human DiGeorge syndrome critical region gene 14 (DGCR14), mGFP tagged 10 ug
$757.00
RC200743L3 Lenti ORF clone of Human DiGeorge syndrome critical region gene 14 (DGCR14), Myc-DDK-tagged 10 ug
$757.00
RC200743L4 Lenti ORF clone of Human DiGeorge syndrome critical region gene 14 (DGCR14), mGFP tagged 10 ug
$757.00
RG200743 DGCR14 (tGFP-tagged) - Human DiGeorge syndrome critical region gene 14 (DGCR14) 10 ug
$657.00
SC112464 DGCR14 (untagged)-Human DiGeorge syndrome critical region gene 14 (DGCR14) 10 ug
$457.00

Plasmid Map for RC200743

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200743
Circular map for RC200743

file-alt Citations

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