MAN2B1 (NM_000528) Human Tagged ORF Clone

SKU RC200638

MAN2B1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MAN2B1
• Synonyms: LAMAN; MANB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200638 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCGCCTACGCGCGGGCTTCGGGGGTCTGCGCTCGCGGCTGCCTGGACTCAGCAGGCCCCTGGACCA; TGTCCCGCGCCCTGCGGCCACCGCTCCCGCCTCTCTGCTTTTTCCTTTTGTTGCTGGCGGCTGCCGGTGC; TCGGGCCGGGGGATACGAGACATGCCCCACAGTGCAGCCGAACATGCTGAACGTGCACCTGCTGCCTCAC; ACACATGATGACGTGGGCTGGCTCAAAACCGTGGACCAGTACTTTTATGGAATCAAGAATGACATCCAGC; ACGCCGGTGTGCAGTACATCCTGGACTCGGTCATCTCTGCCTTGCTGGCAGATCCCACCCGTCGCTTCAT; TTACGTGGAGATTGCCTTCTTCTCCCGTTGGTGGCACCAGCAGACAAATGCCACACAGGAAGTCGTGCGA; GACCTTGTGCGCCAGGGGCGCCTGGAGTTCGCCAATGGTGGCTGGGTGATGAACGATGAGGCAGCCACCC; ACTACGGTGCCATCGTGGACCAGATGACACTTGGGCTGCGCTTTCTGGAGGACACATTTGGCAATGATGG; GCGACCCCGTGTGGCCTGGCACATTGACCCCTTCGGCCACTCTCGGGAGCAGGCCTCGCTGTTTGCGCAG; ATGGGCTTCGACGGCTTCTTCTTTGGGCGCCTTGATTATCAAGATAAGTGGGTACGGATGCAGAAGCTGG; AGATGGAGCAGGTGTGGCGGGCCAGCACCAGCCTGAAGCCCCCGACCGCGGACCTCTTCACTGGTGTGCT; TCCCAATGGTTACAACCCGCCAAGGAATCTGTGCTGGGATGTGCTGTGTGTCGATCAGCCGCTGGTGGAG; GACCCTCGCAGCCCCGAGTACAACGCCAAGGAGCTGGTCGATTACTTCCTAAATGTGGCCACTGCCCAGG; GCCGGTATTACCGCACCAACCACACTGTGATGACCATGGGCTCGGACTTCCAATATGAGAATGCCAACAT; GTGGTTCAAGAACCTTGACAAGCTCATCCGGCTGGTAAATGCGCAGCAGGCAAAAGGAAGCAGTGTCCAT; GTTCTCTACTCCACCCCCGCTTGTTACCTCTGGGAGCTGAACAAGGCCAACCTCACCTGGTCAGTGAAAC; ATGACGACTTCTTCCCTTACGCGGATGGCCCCCACCAGTTCTGGACCGGTTACTTTTCCAGTCGGCCGGC; CCTCAAACGCTACGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAGGCGCTGGTGGGC; CTGGCGGCCAACGTGGGACCCTATGGCTCCGGAGACAGTGCACCCCTCAATGAGGCGATGGCTGTGCTCC; AGCATCACGACGCCGTCAGCGGCACCTCCCGCCAGCACGTGGCCAACGACTACGCGCGCCAGCTTGCGGC; AGGCTGGGGGCCTTGCGAGGTTCTTCTGAGCAACGCGCTGGCGCGGCTCAGAGGCTTCAAAGATCACTTC; ACCTTTTGCCAACAGCTAAACATCAGCATCTGCCCGCTCAGCCAGACGGCGGCGCGCTTCCAGGTCATCG; TTTATAATCCCCTGGGGCGGAAGGTGAATTGGATGGTACGGCTGCCGGTCAGCGAAGGCGTTTTCGTTGT; GAAGGACCCCAATGGCAGGACAGTGCCCAGCGATGTGGTAATATTTCCCAGCTCAGACAGCCAGGCGCAC; CCTCCGGAGCTGCTGTTCTCAGCCTCACTGCCCGCCCTGGGCTTCAGCACCTATTCAGTAGCCCAGGTGC; CTCGCTGGAAGCCCCAGGCCCGCGCACCACAGCCCATCCCCAGAAGATCCTGGTCCCCTGCTTTAACCAT; CGAAAATGAGCACATCCGGGCAACGTTTGATCCTGACACAGGGCTGTTGATGGAGATTATGAACATGAAT; CAGCAACTCCTGCTGCCTGTTCGCCAGACCTTCTTCTGGTACAACGCCAGTATAGGTGACAACGAAAGTG; ACCAGGCCTCAGGTGCCTACATCTTCAGACCCAACCAACAGAAACCGCTGCCTGTGAGCCGCTGGGCTCA; GATCCACCTGGTGAAGACACCCTTGGTGCAGGAGGTGCACCAGAACTTCTCAGCTTGGTGTTCCCAGGTG; GTTCGCCTGTACCCAGGACAGCGGCACCTGGAGCTAGAGTGGTCGGTGGGGCCGATACCTGTGGGCGACA; CCTGGGGGAAGGAGGTCATCAGCCGTTTTGACACACCGCTGGAGACAAAGGGACGCTTCTACACAGACAG; CAATGGCCGGGAGATCCTGGAGAGGAGGCGGGATTATCGACCCACCTGGAAACTGAACCAGACGGAGCCC; GTGGCAGGAAACTACTATCCAGTCAACACCCGGATTTACATCACGGATGGAAACATGCAGCTGACTGTGC; TGACTGACCGCTCCCAGGGGGGCAGCAGCCTGAGAGATGGCTCGCTGGAGCTCATGGTGCACCGAAGGCT; GCTGAAGGACGATGGACGCGGAGTATCGGAGCCACTAATGGAGAACGGGTCGGGGGCGTGGGTGCGAGGG; CGCCACCTGGTGCTGCTGGACACAGCCCAGGCTGCAGCCGCCGGACACCGGCTCCTGGCGGAGCAGGAGG; TCCTGGCCCCTCAGGTGGTGCTGGCCCCGGGTGGCGGCGCCGCCTACAATCTCGGGGCTCCTCCGCGCAC; GCAGTTCTCAGGGCTGCGCAGGGACCTGCCGCCCTCGGTGCACCTGCTCACGCTGGCCAGCTGGGGCCCC; GAAATGGTGCTGCTGCGCTTGGAGCACCAGTTTGCCGTAGGAGAGGATTCCGGACGTAACCTGAGCGCCC; CCGTTACCTTGAACTTGAGGGACCTGTTCTCCACCTTCACCATCACCCGCCTGCAGGAGACCACGCTGGT; GGCCAACCAGCTCCGCGAGGCAGCCTCCAGGCTCAAGTGGACAACAAACACAGGCCCCACACCCCACCAA; ACTCCGTACCAGCTGGACCCGGCCAACATCACGCTGGAACCCATGGAAATCCGCACTTTCCTGGCCTCAG; TTCAATGGAAGGAGGTGGATGGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200638 protein sequence; Red=Cloning site Green=Tags(s); ; MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPH; THDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVR; DLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQ; MGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVE; DPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVH; VLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG; LAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHF; TFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAH; PPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMN; QQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQV; VRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEP; VAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRG; RHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGP; EMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQ; TPYQLDPANITLEPMEIRTFLASVQWKEVDG; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000528
• ORF Size: 3033 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000528.4
• RefSeq Size: 3231 bp
• RefSeq ORF: 3036 bp
• Locus ID: 4125
• UniProt ID: O00754
• Cytogenetics: 19p13.13
• Domains: Glyco_hydro_38
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Other glycan degradation
• MW: 113.7 kDa
• Summary: This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]