PDLIM5 (NM_006457) Human Tagged ORF Clone
SKU
RC200592
PDLIM5 (Myc-DDK-tagged)-Human PDZ and LIM domain 5 (PDLIM5), transcript variant 1
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TrueORF Gold
Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Target Symbol | PDLIM5 |
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Synonyms | ENH; ENH1; L9; LIM |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC200592 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAACTACAGTGTGTCACTGGTTGGCCCAGCTCCTTGGGGTTTCCGGCTGCAGGGCGGTAAGGATT TCAACATGCCTCTGACAATCTCTAGTCTAAAAGATGGCGGCAAGGCAGCCCAGGCAAATGTAAGAATAGG CGATGTGGTTCTCAGCATTGATGGAATAAATGCACAAGGAATGACTCATCTTGAAGCCCAGAATAAGATT AAGGGTTGTACAGGCTCTTTGAATATGACTCTGCAAAGAGCATCTGCTGCACCCAAGCCTGAGCCGGTTC CTGTTCAAAAGGGAGAACCTAAAGAAGTAGTTAAACCTGTGCCCATTACATCTCCTGCTGTGTCCAAAGT CACTTCCACAAACAACATGGCCTACAATAAGGCACCACGGCCTTTTGGTTCTGTGTCTTCACCAAAAGTC ACATCCATCCCATCACCATCGTCTGCCTTCACCCCAGCCCATGCGACCACCTCATCACATGCTTCCCCTT CACCCGTGGCTGCCGTCACTCCTCCCCTGTTCGCTGCATCTGGACTGCATGCTAATGCCAATCTTAGTGC TGACCAGTCTCCATCTGCACTGAGCGCTGGTAAAACTGCAGTTAATGTCCCACGGCAGCCCACAGTCACC AGCGTGTGTTCCGAGACTTCTCAGGAGCTAGCAGAGGGACAGAGAAGAGGATCCCAGGGTGACAGTAAAC AGCAAAATGGCCCACCAAGAAAACACATTGTGGAGCGCTATACAGAGTTTTATCATGTACCCACTCACAG TGATGCCAGCAAGAAGAGACTGATTGAGGATACTGAAGACTGGCGTCCAAGGACTGGAACAACTCAGTCT CGCTCTTTCCGAATCCTTGCCCAGATCACTGGGACTGAACATTTGAAAGAATCTGAAGCCGATAATACAA AGAAGGCAAATAACTCTCAGGAGCCTTCTCCGCAGTTGGCTTCCTCGGTAGCTTCCACACGGAGCATGCC CGAGAGCCTGGACAGCCCAACCTCTGGCAGACCAGGGGTTACCAGCCTCACAACTGCAGCTGCCTTCAAG CCTGTAGGATCCACTGGCGTCATCAAGTCACCAAGCTGGCAACGGCCAAACCAAGGAGTACCTTCCACTG GAAGAATCTCAAACAGCGCTGCTTACTCAGGATCAGTGGCACCAGCCAACTCAGCTTTGGGACAAACCCA GCCAAGTGACCAGGACACTTTAGTGCAAAGAGCTGAGCACATTCCAGCAGGGAAACGAACTCCGATGTGC GCCCATTGTAACCAGGTCATCAGAGGACCATTCTTAGTGGCACTGGGGAAATCTTGGCACCCAGAAGAAT TCAACTGCGCTCACTGCAAAAATACAATGGCCTACATTGGATTTGTAGAGGAGAAAGGAGCCCTGTATTG TGAGCTGTGCTATGAGAAATTCTTTGCCCCTGAATGTGGTCGATGCCAAAGGAAGATCCTTGGAGAAGTC ATCAATGCGTTGAAACAAACTTGGCATGTTTCCTGTTTTGTGTGTGTAGCCTGTGGAAAGCCCATTCGGA ACAATGTTTTTCACTTGGAGGATGGTGAACCCTACTGTGAGACTGATTATTATGCCCTCTTTGGTACTAT ATGCCATGGATGTGAATTTCCCATAGAAGCTGGTGACATGTTCCTGGAAGCTCTGGGCTACACCTGGCAT GACACTTGCTTTGTATGCTCAGTGTGTTGTGAAAGTTTGGAAGGTCAGACCTTTTTCTCCAAGAAGGACA AGCCCCTGTGTAAGAAACATGCTCATTCTGTGAATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200592 protein sequence
Red=Cloning site Green=Tags(s) MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKAAQANVRIGDVVLSIDGINAQGMTHLEAQNKI KGCTGSLNMTLQRASAAPKPEPVPVQKGEPKEVVKPVPITSPAVSKVTSTNNMAYNKAPRPFGSVSSPKV TSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVT SVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKKRLIEDTEDWRPRTGTTQS RSFRILAQITGTEHLKESEADNTKKANNSQEPSPQLASSVASTRSMPESLDSPTSGRPGVTSLTTAAAFK PVGSTGVIKSPSWQRPNQGVPSTGRISNSAAYSGSVAPANSALGQTQPSDQDTLVQRAEHIPAGKRTPMC AHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYEKFFAPECGRCQRKILGEV INALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICHGCEFPIEAGDMFLEALGYTWH DTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006457 |
ORF Size | 1788 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_006457.5 |
RefSeq Size | 6132 bp |
RefSeq ORF | 1791 bp |
Locus ID | 10611 |
UniProt ID | Q96HC4 |
Cytogenetics | 4q22.3 |
Domains | LIM, PDZ |
Protein Families | Druggable Genome |
MW | 64 kDa |
Summary | This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC200592L3 | Lenti ORF clone of Human PDZ and LIM domain 5 (PDLIM5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$855.00
|
|
RC200592L4 | Lenti ORF clone of Human PDZ and LIM domain 5 (PDLIM5), transcript variant 1, mGFP tagged | 10 ug |
$855.00
|
|
RG200592 | PDLIM5 (tGFP-tagged) - Human PDZ and LIM domain 5 (PDLIM5), transcript variant 1 | 10 ug |
$489.00
MSRP
$755.00
MSRP
$755.00
|
|
SC321417 | PDLIM5 (untagged)-Human PDZ and LIM domain 5 (PDLIM5), transcript variant 1 | 10 ug |
$556.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.