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PAN2 (NM_014871) Human Tagged ORF Clone

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SKU
RC200573
PAN2 (Myc-DDK-tagged)-Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,043.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAN2
Synonyms USP52
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200573 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTTGAGGGTCTGGACCCTGGACTGGCAGAATATGCCCCAGCCATGCATTCTGCCCTGGACCCTG
TCTTGGATGCCCACCTGAACCCAAATCTGCTACAGAATGTGGAGCTGGACCCAGAGGGAGTGGCCTTGGA
GGCTCTTCCCGTCCAGGAATCAGTGCACATAATGGAAGGTGTCTACTCTGAATTGCACAGCGTGGTGGCT
GAAGTGGGTGTACCTGTTTCCGTCTCCCACTTTGACTTGCACGAGGAGATGCTGTGGGTGGGGAGCCACG
GGGGCCATGCCACTTCATTTTTTGGCCCAGCCTTGGAGCGCTACTCATCCTTTCAAGTCAATGGCAGTGA
TGATATTCGGCAGATCCAGAGCCTGGAGAATGGTATCCTTTTTCTCACCAAGAACAACCTCAAGTATATG
GCCCGTGGGGGCCTCATTATATTTGATTACCTGCTGGATGAGAATGAGGATATGCACAGTCTCCTACTGA
CTGACAGCAGCACTCTACTCGTTGGTGGGCTGCAGAATCACATACTAGAGATTGATCTTAACACTGTCCA
GGAGACTCAGAAGTATGCAGTAGAGACGCCTGGAGTCACCATCATGAGACAGACAAATCGCTTCTTCTTC
TGCGGCCACACGTCTGGCAAGGTTTCCCTGAGAGACCTCCGTACTTTTAAGGTGGAACATGAGTTTGATG
CCTTCTCAGGAAGTCTGTCAGACTTTGATGTGCATGGCAACCTGCTAGCTGCCTGTGGCTTCTCCAGCCG
CCTCACTGGCCTGGCCTGCGACCGTTTCCTCAAGGTGTATGATTTGCGCATGATGCGTGCCATCACACCA
CTTCAAGTACATGTGGATCCTGCCTTCTTGCGCTTCATTCCTACATATACTTCTCGTCTTGCTATCATCT
CTCAGTCAGGGCAGTGCCAATTCTGTGAACCCACAGGCCTGGCCAACCCAGCCGATATCTTTCATGTGAA
TCCTGTGGGGCCTCTGCTAATGACATTTGATGTGTCAGCCAGCAAGCAGGCTCTGGCCTTTGGGGATTCT
GAGGGCTGTGTGCACCTCTGGACTGATTCCCCGGAGCCTTCCTTCAACCCCTACTCCCGTGAGACTGAGT
TTGCTTTGCCGTGTCTCGTGGACTCACTGCCTCCTCTGGACTGGAGCCAGGACCTGCTGCCTCTTTCCCT
CATCCCTGTCCCACTCACCACTGACACACTTCTCTCTGATTGGCCTGCTGCCAACTCTGCTCCAGCTCCC
AGGCGAGCACCACCCGTGGATGCAGAGATTCTGCGCACCATGAAGAAGGTGGGCTTCATTGGCTATGCGC
CCAATCCCCGCACCAGGCTGCGCAATCAGATACCCTACAGACTCAAGGAGTCAGACAGTGAATTTGACAG
CTTCAGCCAGGTCACTGAGTCACCAGTAGGACGAGAAGAGGAACCACATCTCCACATGGTTTCTAAGAAA
TACCGCAAGGTGACCATCAAATATTCCAAGCTAGGGCTGGAGGACTTTGACTTCAAACACTACAATAAGA
CCTTGTTTGCTGGATTAGAGCCCCACATTCCCAACGCCTACTGTAACTGCATGATCCAGGTGCTCTATTT
CCTGGAGCCTGTACGCTGTCTAATTCAAAACCACCTTTGCCAGAAGGAGTTCTGTCTGGCATGTGAGCTG
GGCTTCCTGTTTCACATGTTGGACCTCTCTCGTGGTGACCCTTGCCAGGGCAATAATTTTCTTCGGGCAT
TCCGTACTATTCCTGAGGCCTCAGCCCTCGGTCTAATCCTGGCTGACTCAGATGAGGCCTCAGGCAAGGG
CAATCTGGCCAGGCTCATTCAGAGGTGGAATCGCTTCATTCTCACTCAACTGCATCAAGATATGCAGGAG
CTGGAAATACCACAGGCTTATCGAGGTGCTGGAGGCAGCAGCTTTTGCTCATCGGGGGACTCTGTTATTG
GGCAGCTCTTCAGCTGTGAGATGGAGAACTGCAGCCTCTGCCGCTGTGGCAGTGAGACCGTGCGAGCCTC
ATCCACTCTGCTTTTCACACTCTCCTACCCTGATGATAAAACTGGGAAGAACTATGACTTTGCTCAGGTG
CTGAAGCGAAGCATCTGCCTGGACCAGAATACACAGGCCTGGTGTGACACCTGTGAAAAGTACCAGCCCA
CGATTCAGACCCGCAACATCCGCCATCTGCCAGATATTCTTGTCATCAATTGTGAGGTGAACAGCTCAAA
AGAGGCTGATTTCTGGAGAATGCAGGCTGAGGTTGCCTTCAAGATGGCAGTAAAGAAACACGGTGGGGAA
ATCTCCAAGAACAAGGAATTTGCTTTGGCTGATTGGAAGGAACTAGGGAGTCCAGAGGGTGTGCTGGTGT
GTCCCTCCATTGAGGAGTTGAAGAACGTCTGGCTTCCTTTCTCCATTCGCATGAAGATGACCAAAAACAA
AGGGCTGGATGTTTGCAATTGGACTGATGGGGATGAGATGCAGTGGGGCCCAGCCAGGGCAGAGGAGGAG
CATGGTGTCTATGTGTATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGCACAGGGGGCAGCC
TGGTGGCTCACATCAAAGTTGGAGAGACCTACCACCAGCGCAAGGAGGGCGTTACTCACCAGCAGTGGTA
TCTGTTCAATGACTTTCTTATTGAACCTATTGATAAGCATGAAGCTGTGCAGTTTGACATGAATTGGAAA
GTACCTGCAATCCTTTATTATGTCAAACGGAATCTCAATTCCAGATACAACCTGAACATCAAGAACCCTA
TTGAGGCAAGTGTCTTGCTGGCTGAAGCCTCGCTGGCACGGAAGCAGCGGAAAACACATACTACCTTTAT
TCCACTGATGCTGAATGAGATGCCACAGATTGGGGACCTGGTGGGTCTGGATGCTGAGTTTGTCACCCTT
AATGAGGAGGAAGCAGAGTTACGCAGTGATGGTACCAAGTCTACCATTAAACCAAGCCAGATGTCAGTAG
CCAGGATTACCTGTGTTCGGGGCCAGGGACCCAATGAGGGTATCCCCTTCATTGATGACTACATCTCTAC
CCAGGAGCAGGTGGTGGATTACTTGACTCAATACTCGGGTATAAAGCCTGGTGACCTCGATGCCAAAATT
TCCTCCAAGCACCTAACAACTCTCAAGTCTACCTACTTAAAGCTTCGTTTTCTCATTGACATTGGAGTCA
AGTTTGTGGGTCATGGCCTGCAGAAGGACTTCCGGGTCATCAACCTGATGGTGCCCAAGGACCAAGTCCT
TGACACTGTCTACCTGTTCCATATGCCCCGAAAACGAATGATTTCCCTGCGATTCCTTGCTTGGTACTTT
CTGGACCTGAAGATTCAAGGGGAAACCCATGACAGTATTGAGGATGCCCGCACAGCCCTTCAGCTGTACC
GAAAGTATCTGGAGCTAAGCAAAAATGGCACTGAGCCTGAGTCTTTCCACAAGGTGCTCAAGGGTCTTTA
TGAGAAGGGCAGAAAGATGGACTGGAAGGTGCCTGAGCCTGAGGGCCAAACAAGTCCCAAGAATGCAGCT
GTCTTCTCCTCAGTGCTGGCGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200573 protein sequence
Red=Cloning site Green=Tags(s)

MNFEGLDPGLAEYAPAMHSALDPVLDAHLNPNLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVA
EVGVPVSVSHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGSDDIRQIQSLENGILFLTKNNLKYM
ARGGLIIFDYLLDENEDMHSLLLTDSSTLLVGGLQNHILEIDLNTVQETQKYAVETPGVTIMRQTNRFFF
CGHTSGKVSLRDLRTFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITP
LQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDS
EGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDTLLSDWPAANSAPAP
RRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDSEFDSFSQVTESPVGREEEPHLHMVSKK
YRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACEL
GFLFHMLDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDMQE
LEIPQAYRGAGGSSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPDDKTGKNYDFAQV
LKRSICLDQNTQAWCDTCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRMQAEVAFKMAVKKHGGE
ISKNKEFALADWKELGSPEGVLVCPSIEELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPARAEEE
HGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWK
VPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVGLDAEFVTL
NEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKI
SSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLAWYF
LDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAA
VFSSVLAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014871
ORF Size 3594 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014871.2, NP_055686.2
RefSeq Size 5289 bp
RefSeq ORF 3597 bp
Locus ID 9924
UniProt ID Q504Q3
Cytogenetics 12q13.3
Domains UCH
Protein Families Protease
MW 135 kDa
Summary This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants. provided by RefSeq, Oct 2009
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"NM_014871" in other vectors (4)
SKU Description Size Price
RC200573L3 Lenti ORF clone of Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 3, Myc-DDK-tagged 10 ug
$1,343.00
RC200573L4 Lenti ORF clone of Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 3, mGFP tagged 10 ug
$1,343.00
RG200573 PAN2 (tGFP-tagged) - Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 3 10 ug
$789.00 $1,243.00
SC319535 PAN2 (untagged)-Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 3 10 ug
$1,044.00

Plasmid Map for RC200573

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200573
Circular map for RC200573

file-alt Citations

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