GNG5 (NM_005274) Human Tagged Lenti ORF Clone
- LentiORF®
SKU
RC200572L4
Lenti ORF clone of Human guanine nucleotide binding protein (G protein), gamma 5 (GNG5), mGFP tagged
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GNG5 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005274 |
| ORF Size | 204 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005274.1 |
| RefSeq Size | 823 bp |
| RefSeq ORF | 207 bp |
| Locus ID | 2787 |
| UniProt ID | P63218 |
| Cytogenetics | 1p22.3 |
| Domains | G-gamma |
| Protein Pathways | Chemokine signaling pathway |
| MW | 7.3 kDa |
| Summary | G proteins are trimeric (alpha-beta-gamma) membrane-associated proteins that regulate flow of information from cell surface receptors to a variety of internal metabolic effectors. Interaction of a G protein with its activated receptor promotes exchange of GTP for GDP that is bound to the alpha subunit. The alpha-GTP complex dissociates from the beta-gamma heterodimer so that the subunits, in turn, may interact with and regulate effector molecules (Gilman, 1987 PubMed 3113327; summary by Ahmad et al., 1995) PubMed 7606925.supplied by OMIM, Nov 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005274" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200572 | GNG5 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), gamma 5 (GNG5) | 10 ug |
$150.00
|
|
| RC200572L3 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), gamma 5 (GNG5), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RG200572 | GNG5 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), gamma 5 (GNG5) | 10 ug |
$489.00
|
|
| SC116833 | GNG5 (untagged)-Human guanine nucleotide binding protein (G protein), gamma 5 (GNG5) | 10 ug |
$150.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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