GNG5 (NM_005274) Human Untagged Clone

SKU SC116833

GNG5 (untagged)-Human guanine nucleotide binding protein (G protein), gamma 5 (GNG5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GNG5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005274 edited; ATGTCTGGCTCCTCCAGCGTCGCCGCTATGAAGAAAGTGGTTCAACAGCTCCGGCTGGAG; GCCGGACTCAACCGCGTAAAAGTTTCCCAGGCAGCTGCAGACTTGAAACAGTTCTGTCTG; CAGAATGCTCAACATGACCCTCTGCTGACTGGAGTATCTTCAAGTACAAATCCCTTCAGA; CCCCAGAAAGTCTGTTCCTTTTTGTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005274 unedited; TCATATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGCACGAATCG; GCCCGGCCGTCGCGTGCACCATGTCTGGCTCCTCCAGCGTCGCCGCTATGAAGAAAGTGG; TTCAACAGCTCCGGCTGGAGGCCGGACTCAACCGCGTAAAAGTTTCCCAGGCAGCTGCAG; ACTTGAAACAGTTCTGTCTGCAGAATGCTCAACATGACCCTCTGCTGACTGGAGTATCTT; CAAGTACAAATCCCTTCAGACCCCAGAAAGTCTGTTCCTTTTTGTAGTAAAATGAATCTT; TCAAAGGTTTCCCAAACCACTCCTTATGATCCAGTGAATATTCAAGAGAGCTACATTTGA; AGCCTGTACAAAAGCTTATCCCTGTAACACATGTGCCATAATATACAAACTTCTACTTTC; GTCAGTCCTTAACATCTACCTCTCTGAATTTTCATGAATTTCTATTTCACAAGGGTAATT; GTTTTATATACACTGGCAGCAGCATACAATAAAACTTAGTATGAAACTTTAAAAAAAAAA; AAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCG; GGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAG; TGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCT; TCTTTNTATTATGGGGTGAGGGGGTTGGGGTTTGGGNACCAAGGGGCAAGTTGGGGAAAA; CAACCTGTANGGCCTGCGGGGTCTATTGGGAACCAGCTGGAGTGCAGTGGCACAATCTTG; GCTACTGGCATCTCGCTNCTGGGGTCAAGCGATCTCTGCTCAGCTCCGAGTNGTTGGGAT; TCCAGCTGCTGACCAGCTACTATTTTTGTTTTTTTGATAAAACAGGGTTTACATATGCCA; GNCTGTNCTCACTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005274 unedited; GGGGGGCGAGGACGAACCTTTTTNNNNNNNNTTTCTTGNNACCCGGCCGCATTTANGATC; GGTTTTTTTTTTTTTTTTTTTAAGTTTCTACTAAGTTTTATTGTATGCTGCTGCCAGTGT; ATATAAAACAATTACCCTTGTGAAATAGAAATTCATGAAAATTCAGAGAGGTAGATGTTA; AGGACTGACGAAAGTAGAAGTTTGTATATTATGGCACATGTGTTACAGGGATAAGCTTTT; GTACAGGCTTCAAATGTAGCTCTCTTGAATATTCACTGGATCATAAGGAGTGGTTTGGGA; AACCTTTGAAAGATTCATTTTACTACAAAAAGGAACAGACTTTCTGGGGTCTGAAGGGAT; TTGTACTTGAAGATACTCCAGTCAGCAGAGGGTCATGTTGAGCATTCTGCAGACAGAACT; GTTTCAAGTCTGCAGCTGCCTGGGAAACTTTTACGCGGTTGAGTCCGGCCTCCAGCCGGA; GCTGTTGAACCACTTTCTTCATAGCGGCGACGCTGGAGGAGCCAGACATGGTGCACGCGA; CGGCCGGGCCGATTCGTGCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTAC; AAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCT; ACCGNCCATTTGCGTCAACGGGGCGGNGTTTATACGACATTTTNGAAAGTCCCCGTGATT; TTTGTGCCAAAACAACTTCCATTGACGTCAATGGGGTGGAGACTTNGNAATCCCCGTGAG; TCAAACCGTATCCACGCCCATTGTGTACTGCCAAACCGCATCACATGGTAATAGCGATGA; CTAATACGTAAATGACTGCCAAGTAGAAAGTCCGGAAGGTCATGTACTGGGCATAAGGCA; GGCGGGCCATTAACCGCATTGACGTCAAAGGGGGCGGACTTGCTTATAAACACTTGAGTC; CTGCCAAGTGCGCATTTACCCGGAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005274
• Insert Size: 570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005274.1, NP_005265.1
• RefSeq Size: 698 bp
• RefSeq ORF: 207 bp
• Locus ID: 2787
• UniProt ID: P63218
• Cytogenetics: 1p22.3
• Domains: G-gamma
• Protein Pathways: Chemokine signaling pathway
• Summary: G proteins are trimeric (alpha-beta-gamma) membrane-associated proteins that regulate flow of information from cell surface receptors to a variety of internal metabolic effectors. Interaction of a G protein with its activated receptor promotes exchange of GTP for GDP that is bound to the alpha subunit. The alpha-GTP complex dissociates from the beta-gamma heterodimer so that the subunits, in turn, may interact with and regulate effector molecules (Gilman, 1987 [PubMed 3113327]; summary by Ahmad et al., 1995) [PubMed 7606925].[supplied by OMIM, Nov 2010]