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BIK (NM_001197) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200546
BIK (Myc-DDK-tagged)-Human BCL2-interacting killer (apoptosis-inducing) (BIK)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "BIK or NM_001197"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BIK
Synonyms BIP1; BP4; NBK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200546 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGAAGTAAGACCCCTCTCCAGAGACATCTTGATGGAGACCCTCCTGTATGAGCAGCTCCTGGAAC
CCCCGACCATGGAGGTTCTTGGCATGACTGACTCTGAAGAGGACCTGGACCCTATGGAGGACTTCGATTC
TTTGGAATGCATGGAGGGCAGTGACGCATTGGCCCTGCGGCTGGCCTGCATCGGGGACGAGATGGACGTG
AGCCTCAGGGCCCCGCGCCTGGCCCAGCTCTCCGAGGTGGCCATGCACAGCCTGGGTCTGGCTTTCATCT
ACGACCAGACTGAGGACATCAGGGATGTTCTTAGAAGTTTCATGGACGGTTTCACCACACTTAAGGAGAA
CATAATGAGGTTCTGGAGATCCCCGAACCCCGGGTCCTGGGTGTCCTGCGAACAGGTGCTGCTGGCGCTG
CTGCTGCTGCTGGCGCTGCTGCTGCCGCTGCTCAGCGGGGGCCTGCACCTGCTGCTCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200546 protein sequence
Red=Cloning site Green=Tags(s)

MSEVRPLSRDILMETLLYEQLLEPPTMEVLGMTDSEEDLDPMEDFDSLECMEGSDALALRLACIGDEMDV
SLRAPRLAQLSEVAMHSLGLAFIYDQTEDIRDVLRSFMDGFTTLKENIMRFWRSPNPGSWVSCEQVLLAL
LLLLALLLPLLSGGLHLLLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001197
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001197.5
RefSeq Size 966 bp
RefSeq ORF 483 bp
Locus ID 638
UniProt ID Q13323
Cytogenetics 22q13.2
Protein Families Druggable Genome, Stem cell - Pluripotency, Transmembrane
MW 18 kDa
Summary The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. provided by RefSeq, Sep 2011
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Documents
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"NM_001197" in other vectors (6)
SKU Description Size Price
RC200546L1 Lenti ORF clone of Human BCL2-interacting killer (apoptosis-inducing) (BIK), Myc-DDK-tagged 10 ug
$450.00
RC200546L2 Lenti ORF clone of Human BCL2-interacting killer (apoptosis-inducing) (BIK), mGFP tagged 10 ug
$450.00
RC200546L3 Lenti ORF clone of Human BCL2-interacting killer (apoptosis-inducing) (BIK), Myc-DDK-tagged 10 ug
$450.00
RC200546L4 Lenti ORF clone of Human BCL2-interacting killer (apoptosis-inducing) (BIK), mGFP tagged 10 ug
$450.00
RG200546 BIK (tGFP-tagged) - Human BCL2-interacting killer (apoptosis-inducing) (BIK) 10 ug
$489.00
SC119390 BIK (untagged)-Human BCL2-interacting killer (apoptosis-inducing) (BIK) 10 ug
$150.00

Plasmid Map for RC200546

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200546
Circular map for RC200546

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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