HEXIM1 (NM_006460) Human Tagged ORF Clone

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SKU
RC200536
HEXIM1 (Myc-DDK-tagged)-Human hexamethylene bis-acetamide inducible 1 (HEXIM1)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HEXIM1
Synonyms CLP1; EDG1; HIS1; MAQ1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200536 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGCCATTCTTGTCAGAATATCAACACCAGCCTCAAACTAGCAACTGTACAGGTGCTGCTGCTG
TCCAGGAAGAGCTGAACCCTGAGCGCCCCCCAGGCGCGGAGGAGCGGGTGCCCGAGGAGGACAGTAGGTG
GCAATCGAGAGCGTTCCCCCAGTTGGGTGGCCGTCCGGGGCCGGAGGGGGAAGGGAGCCTGGAATCCCAA
CCACCTCCCTTGCAGACCCAGGCCTGTCCAGAATCTAGCTGCCTGAGAGAGGGCGAGAAGGGCCAGAATG
GGGACGACTCGTCCGCTGGCGGCGACTTCCCGCCGCCGGCAGAAGTGGAGCCGACGCCCGAGGCCGAGCT
GCTCGCCCAGCCTTGTCATGACTCCGAGGCCAGTAAGTTGGGGGCTCCTGCCGCAGGGGGCGAAGAGGAG
TGGGGACAGCAGCAGAGACAGCTGGGGAAGAAAAAACATAGGAGACGCCCGTCCAAGAAGAAGCGGCATT
GGAAACCGTACTACAAGCTGACCTGGGAAGAGAAGAAAAAGTTCGACGAGAAACAGAGCCTTCGAGCTTC
AAGGATCCGAGCCGAGATGTTCGCCAAGGGCCAGCCGGTCGCGCCCTATAACACCACGCAGTTCCTCATG
GATGATCACGACCAGGAGGAGCCGGACCTCAAAACCGGCCTGTACTCCAAGCGGGCCGCCGCCAAATCCG
ACGACACCAGCGATGACGACTTCATGGAAGAAGGGGGTGAGGAGGATGGGGGCAGCGATGGGATGGGAGG
GGACGGCAGCGAGTTTCTGCAGCGGGACTTCTCGGAGACGTACGAGCGGTACCACACGGAGAGCCTGCAG
AACATGAGCAAGCAGGAGCTCATCAAGGAGTACCTGGAACTGGAGAAGTGCCTCTCGCGCATGGAGGACG
AGAACAACCGGCTGCGGCTGGAGAGCAAGCGGCTGGGTGGCGACGACGCGCGTGTGCGGGAGCTGGAGCT
GGAGCTGGACCGGCTGCGCGCCGAGAACCTCCAGCTGCTGACCGAGAACGAACTGCACCGGCAGCAGGAG
CGAGCGCCGCTTTCCAAGTTTGGAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200536 protein sequence
Red=Cloning site Green=Tags(s)

MAEPFLSEYQHQPQTSNCTGAAAVQEELNPERPPGAEERVPEEDSRWQSRAFPQLGGRPGPEGEGSLESQ
PPPLQTQACPESSCLREGEKGQNGDDSSAGGDFPPPAEVEPTPEAELLAQPCHDSEASKLGAPAAGGEEE
WGQQQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKKFDEKQSLRASRIRAEMFAKGQPVAPYNTTQFLM
DDHDQEEPDLKTGLYSKRAAAKSDDTSDDDFMEEGGEEDGGSDGMGGDGSEFLQRDFSETYERYHTESLQ
NMSKQELIKEYLELEKCLSRMEDENNRLRLESKRLGGDDARVRELELELDRLRAENLQLLTENELHRQQE
RAPLSKFGD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006460
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006460.3
RefSeq Size 4785 bp
RefSeq ORF 1080 bp
Locus ID 10614
UniProt ID O94992
Cytogenetics 17q21.31
Protein Families Transcription Factors
MW 40.6 kDa
Summary Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006460" in other vectors (5)
SKU Description Size Price
RC200536L3 Lenti ORF clone of Human hexamethylene bis-acetamide inducible 1 (HEXIM1), Myc-DDK-tagged 10 ug
$757.00
RC200536L4 Lenti ORF clone of Human hexamethylene bis-acetamide inducible 1 (HEXIM1), mGFP tagged 10 ug
$757.00
RG200536 HEXIM1 (tGFP-tagged) - Human hexamethylene bis-acetamide inducible 1 (HEXIM1) 10 ug
$489.00 $657.00
SC127947 HEXIM1 (untagged)-Human hexamethylene bis-acetamide inducible 1 (HEXIM1) 10 ug
$457.00
SC320724 HEXIM1 (untagged)-Human hexamethylene bis-acetamide inducible 1 (HEXIM1) 10 ug
$457.00

Plasmid Map for RC200536

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Cloning scheme for RC200536

Circular map for RC200536

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