MCL1 (NM_021960) Human Tagged ORF Clone
SKU
RC200521
MCL1 (Myc-DDK-tagged)-Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MCL1 |
| Synonyms | bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_021960 |
| ORF Size | 1050 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_021960.5 |
| RefSeq Size | 4020 bp |
| RefSeq ORF | 1053 bp |
| Locus ID | 4170 |
| UniProt ID | Q07820 |
| Cytogenetics | 1q21.2 |
| Domains | Bcl-2 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 37.2 kDa |
| Summary | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. provided by RefSeq, Oct 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_021960" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200521L1 | Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC200521L2 | Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC200521L3 | Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC200521L4 | Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG200521 | MCL1 (tGFP-tagged) - Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$886.00
|
|
| SC315538 | MCL1 (untagged)-Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$686.00
|
|
Citations
Oleuropein regulates ubiquitination-mediated Mcl-1 turnover and exhibits antitumor activity
Liu, Peng, Liao et al
Cancer Gene Ther (2025)
Liu, Peng, Liao et al
Cancer Gene Ther (2025)
Cited by 1
AI Summary: OriGene’s Flag-Mcl-1 WT plasmid (#RC200521) was used in this study to investigate the regulation and degradation of Mcl-1 by oleuropein in oral squamous cell carcinoma cells.
Snippet: Flag-Mcl-1 WT (#RC200521) was purchased from OriGene and Flag-Mcl-1-5KR was constructed by our research group [36]. 293 T cells were used to co-transfect PSPAX2
MCL1 inhibition: a promising approach to augment the efficacy of sorafenib in NSCLC through ferroptosis induction
Huang, Chen, Chen et al
Cell Death Discov (2024) 10 (1), 137 DOI: 10.1038/s41420-024-01908-5
Huang, Chen, Chen et al
Cell Death Discov (2024) 10 (1), 137 DOI: 10.1038/s41420-024-01908-5
Cited by 11
AI Summary: OriGene’s Human pCMV6-MCL1-Myc-DDK plasmid (RC200521) was used in this study to manipulate MCL1 expression, enabling the investigation of MCL1’s role in ferroptosis and the efficacy of sorafenib in non-small cell lung cancer.
Snippet: Biological Industries BII03-033-1B Fetal bovine serum Biological Industries 04-001-1A EZPCR Mycoplasma Test Kit Biological Industries 20-700-20 Human pCMV6-MCL1-Myc-DDK plasmid OriGene RC200521 Lipofectamine 3000 reagent Thermo Fisher Scientific L3000-015 Sorafenib MedChemExpress HY-10201A Liproxstatin-1 (Liprox-1) TargetMol T2376 Deferoxamine mesyl
MCL1 Inhibition: A Promising Approach to Augment the Efficacy of Sorafenib in NSCLC through Ferroptosis Induction
Hong, Huang, Chen et al
Research Square (2023)
Hong, Huang, Chen et al
Research Square (2023)
Stabilization of MCL-1 by E3 ligase TRAF4 confers radioresistance
Li, Gao, Li et al
Cell Death Dis (2022) 13 (12), 1053 DOI: 10.1038/s41419-022-05500-6
Li, Gao, Li et al
Cell Death Dis (2022) 13 (12), 1053 DOI: 10.1038/s41419-022-05500-6
Cited by 26
AI Summary: OriGene products Flag-TRAF4 (Cat# RC200345) and Flag-MCL1 (Cat# RC200521) were used in this study to investigate the roles of TRAF4 and MCL-1 in mediating radioresistance in oral squamous cell carcinoma.
Snippet: : 1:200), HA tag (#ab18181, 1:5000) and His tag (#ab18184, 1:5000) were purchased from Abcam (Cambridge, UK). Plasmid construction Flag-TRAF4 (#RC200345) and Flag-MCL1 (#RC200521) were obtained from OriGene Technologies, Inc. His-Ub (#31815), HA-Akt (#73408), HA-Ub-K48 (#17605), and Flag-JOSD1 (#22547) were obtained from Addgene (Watertown, MA, USA). Flag-TRAF4 (C18A), HA-Ub-K48R, His-Ub (K6, K11
SHP-1/STAT3-Signaling-Axis-Regulated Coupling between BECN1 and SLC7A11 Contributes to Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma
Huang, Chen, Chen et al
Int J Mol Sci (2022) 23 (19) DOI: 10.3390/ijms231911092
Huang, Chen, Chen et al
Int J Mol Sci (2022) 23 (19) DOI: 10.3390/ijms231911092
Cited by 54
AI Summary: OriGene products contributed to this study by providing the BECN1 (SR311964) and MCL1 (SR307374) siRNAs used for gene silencing experiments to investigate the molecular mechanisms underlying sorafenib-induced ferroptosis in hepatocellular carcinoma.
SHP-1/STAT3-Signaling-Axis-Regulated Coupling between BECN1 and SLC7A11 Contributes to Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma
Huang, Chen, Chen et al
IJMS (2022) 23 (19), 11092 DOI: 10.3390/ijms231911092
Huang, Chen, Chen et al
IJMS (2022) 23 (19), 11092 DOI: 10.3390/ijms231911092
Cited by 54
AI Summary: OriGene products—specifically the pCMV6-SHP-1-Myc-DDK plasmid (RC213896), pCMV6-STAT3-Myc-DDK plasmid (RC215836), and pCMV6-MCL1-Myc-DDK plasmid (RC200521)—were used to ectopically express SHP-1, STAT3, and MCL1, enabling the authors to investigate the molecular mechanisms underlying sorafenib-induced ferroptosis in hepatocellular carcinoma.
Snippet: were obtained from Dharmacon (Lafayette, CO, USA). Human pCMV6-SHP-1-Myc-DDK plasmid (RC213896), pCMV6-STAT3-Myc-DDK plasmid (RC215836), and pCMV6-MCL1-Myc-DDK plasmid (RC200521) were purchased from OriGene (Rockville, MD, USA). Lipofectamine 3000 reagent (L3000-015), C11-BODIPY (D3861), and RediPlate 96 EnzChek Tyrosine Phosphatase Assay kits (R22067) were obtained from Thermo Fisher Scientific
Showing 1-6 of 21 papers.
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