AKAP1 (NM_003488) Human Tagged ORF Clone

SKU RC200506

AKAP1 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 1 (AKAP1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: AKAP1
• Synonyms: AKAP; AKAP84; AKAP121; AKAP149; D-AKAP1; PPP1R43; PRKA1; SAKAP84; TDRD17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200506 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAATCCAGTTCCGTTCGCTCTTCCCCTTGGCATTGCCTGGGATGCTGGCGCTCCTCGGCTGGTGGT; GGTTTTTCTCTCGTAAAAAAGGCCATGTCAGCAGCCATGATGAGCAGCAGGTGGAGGCTGGTGCTGTGCA; GCTGAGGGCTGACCCTGCCATCAAGGAACCTCTCCCCGTGGAAGACGTCTGTCCCAAAGTAGTGTCCACA; CCCCCCAGTGTCACAGAGCCTCCAGAAAAGGAACTGTCCACCGTGAGCAAGCTGCCTGCAGAGCCCCCAG; CATTGCTCCAGACACACCCACCTTGCCGAAGATCAGAGTCCTCGGGCATTCTTCCTAACACCACAGACAT; GAGATTGCGACCAGGAACACGCAGAGATGACAGTACAAAGCTGGAGCTAGCCCTGACAGGTGGTGAAGCC; AAATCGATTCCTCTAGAGTGCCCCCTTTCATCCCCAAAGGGTGTACTATTCTCCAGCAAATCAGCTGAGG; TGTGTAAGCAAGATTCCCCCTTCAGCAGGGTGCCAAGGAAGGTCCAGCCAGGCTACCCCGTAGTCCCCGC; AGAGAAGCGTAGCTCTGGGGAGAGGGCAAGAGAGACAGGTGGGGCCGAAGGGACTGGTGATGCCGTGTTG; GGGGAAAAGGTGCTTGAAGAAGCTCTGTTGTCTCGGGAGCATGTCTTGGAATTGGAGAACAGCAAGGGCC; CCAGCCTGGCCTCTTTAGAGGGGGAAGAAGATAAGGGGAAGAGCAGCTCATCCCAGGTGGTGGGGCCAGT; GCAGGAGGAAGAGTATGTAGCAGAGAAGTTGCCAAGTAGGTTCATCGAGTCGGCTCACACAGAGCTGGCA; AAGGACGATGCGGCGCCAGCACCCCCAGTCGCAGACGCCAAAGCCCAGGATAGAGGTGTCGAGGGAGAAC; TGGGCAATGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAA; TGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGATTAAGCGGGCTGCCTTCCAG; ATAATCTCCCAAGTGATCTCAGAAGCAACCGAACAGGTGCTGGCCACCACGGTTGGCAAGGTTGCAGGTC; GTGTGTGTCAGGCCAGTCAGCTCCAAGGGCAGAAGGAAGAGAGCTGTGTCCCAGTTCACCAGAAAACTGT; CTTGGGCCCAGACACTGCGGAGCCTGCCACAGCAGAGGCAGCTGTTGCCCCGCCGGATGCTGGCCTCCCC; TTGCCAGGCCTACCAGCAGAGGGCTCACCACCACCAAAGACCTACGTGAGCTGCCTGAAGAGCCTTCTGT; CCAGCCCCACCAAGGACAGTAAGCCAAATATCTCTGCACACCACATCTCCCTGGCCTCCTGCCTGGCACT; GACCACCCCCAGTGAAGAGTTGCCGGACCGGGCAGGCATCCTGGTGGAAGATGCCACCTGTGTCACCTGC; ATGTCAGACAGCAGCCAAAGTGTCCCTTTGGTGGCTTCTCCAGGACACTGCTCAGATTCTTTCAGCACTT; CAGGGCTTGAAGACTCTTGCACAGAGACCAGCTCGAGCCCCAGGGACAAGGCCATCACCCCGCCACTGCC; AGAAAGTACTGTGCCCTTCAGCAATGGGGTGCTGAAGGGGGAGTTGTCAGACTTGGGGGCTGAGGATGGA; TGGACCATGGATGCGGAAGCAGATCATTCAGGAGGTTCTGACAGGAACAGCATGGATTCCGTGGATAGCT; GTTGCAGTCTCAAGAAGACTGAGAGCTTCCAAAATGCCCAGGCAGGCTCCAACCCTAAGAAGGTCGACCT; CATCATCTGGGAGATCGAGGTGCCAAAGCACTTAGTCGGTCGGCTAATTGGCAAGCAGGGGCGCTATGTG; AGTTTTCTGAAGCAAACATCTGGTGCCAAGATCTACATTTCAACCCTGCCTTACACCCAGAGCGTCCAGA; TCTGCCACATAGAAGGCTCTCAACATCATGTAGACAAAGCGCTGAACTTGATTGGGAAGAAGTTCAAAGA; GCTGAACCTCACCAATATCTACGCTCCCCCATTGCCTTCACTGGCACTGCCTTCTCTGCCGATGACATCC; TGGCTCATGCTGCCTGATGGCATCACCGTGGAGGTCATTGTGGTCAACCAGGTCAATGCCGGGCACCTGT; TCGTGCAGCAGCACACACACCCTACCTTCCACGCGCTGCGCAGCCTCGACCAGCAGATGTACCTCTGTTA; CTCTCAGCCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATAACGGTCATCTGTGCCGCCCCTGGTGCG; GACGGGGCCTGGTGGCGAGCCCAAGTGGTTGCCTCCTACGAGGAGACCAACGAAGTGGAGATTCGATACG; TGCACTACGGCGGATATAAGAGGGTGAAAGTAGACGTGCTCCGGCAAATCAGGTCTGACTTTGTCACCCT; GCCGTTTCAGGGAGCAGAAGTCCTTCTGGACAGTGTGATGCCCCTGTCAGACGATGACCAGTTTTCACCG; GAAGCAGATGCCGCCATGAGCGAGATGACGGGGAATACAGCACTGCTTGCTCAGGTGACAAGTTACAGTC; CAACTGGTCTTCCTCTGATTCAGCTGTGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCCCT; GGTGGAGCGAGGCCTTGCCCAGTGGGTAGACAGCTACTACACAAGCCTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200506 protein sequence; Red=Cloning site Green=Tags(s); ; MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVST; PPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEA; KSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVL; GEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELA; KDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQ; IISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLP; LPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVEDATCVTC; MSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDG; WTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYV; SFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTS; WLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGA; DGAWWRAQVVASYEETNEVEIRYVHYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSP; EADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYYTSL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003488
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003488.4
• RefSeq Size: 3968 bp
• RefSeq ORF: 2712 bp
• Locus ID: 8165
• UniProt ID: Q92667
• Cytogenetics: 17q22
• Domains: KH, TUDOR
• Protein Families: Druggable Genome, Transmembrane
• MW: 97.4 kDa
• Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008]