IRF5 (NM_032643) Human Tagged ORF Clone
SKU
RC200458
IRF5 (Myc-DDK-tagged)-Human interferon regulatory factor 5 (IRF5), transcript variant 2
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IRF5 |
| Synonyms | SLEB10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_032643 |
| ORF Size | 1494 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032643.4 |
| RefSeq Size | 2778 bp |
| RefSeq ORF | 1497 bp |
| Locus ID | 3663 |
| UniProt ID | Q13568 |
| Cytogenetics | 7q32.1 |
| Domains | IRF |
| Protein Families | Transcription Factors |
| Protein Pathways | Toll-like receptor signaling pathway |
| MW | 56 kDa |
| Summary | This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. provided by RefSeq, Dec 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_032643" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200458L1 | Lenti ORF clone of Human interferon regulatory factor 5 (IRF5), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200458L2 | Lenti ORF clone of Human interferon regulatory factor 5 (IRF5), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RC200458L3 | Lenti ORF clone of Human interferon regulatory factor 5 (IRF5), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200458L4 | Lenti ORF clone of Human interferon regulatory factor 5 (IRF5), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RG200458 | IRF5 (tGFP-tagged) - Human interferon regulatory factor 5 (IRF5), transcript variant 2 | 10 ug |
$489.00
$657.00
|
|
| SC104269 | IRF5 (untagged)-Human interferon regulatory factor 5 (IRF5), transcript variant 2 | 10 ug |
$457.00
|
|
Citations
Overexpression of miR-133a-3p inhibits fibrosis and proliferation of keloid fibroblasts by regulating IRF5 to inhibit the TGF-β/Smad2 pathway
Huang, Wang, Lin et al
Mol Cell Probes (2020) 52, 101563 DOI: 10.1016/j.mcp.2020.101563
Huang, Wang, Lin et al
Mol Cell Probes (2020) 52, 101563 DOI: 10.1016/j.mcp.2020.101563
Cited by 24
AI Summary: In this study, OriGene’s IRF5 shRNA lentiviral particles (Cat# TL316557V) and IRF5 overexpression plasmid (Cat# RC204393) were used to manipulate IRF5 expression in keloid fibroblasts, enabling the authors to demonstrate that miR-133a-3p inhibits fibrosis and proliferation by targeting IRF5 and modulating the TGF-β/Smad2 pathway.
Overexpression of miR-133a-3p inhibits fibrosis and proliferation of keloid fibroblasts by regulating IRF5 to inhibit the TGF-β/Smad2 pathway
Huang, Wang, Lin et al
Mol. Cell. Probes (2020) 52, 101563 DOI: 10.1016/j.mcp.2020.101563
Huang, Wang, Lin et al
Mol. Cell. Probes (2020) 52, 101563 DOI: 10.1016/j.mcp.2020.101563
Cited by 24
AI Summary: OriGene’s IRF5 plasmid (CAT#: RC200458) was used in this study to overexpress IRF5 in fibroblasts, enabling the authors to investigate and confirm the regulatory relationship between miR-133a-3p and IRF5 in keloid fibroblast fibrosis and proliferation.
Snippet: To determine if IRF5 is regulated by miR-133a-3p, miR-133a-3p mimic control and IRF5 (contained its own 3′UTR) plasmids (CAT#: RC200458, OriGene, USA) or miR-133a-3p mimic and IRF5 plasmids were co-transfected into fibroblasts;- To determine if IRF5 is regulated by miR-133a-3p, miR-133a-3p mimic control and IRF5 (contained its own 3′UTR) plasmids (CAT#: RC200458, OriGene, USA) or miR-133a-3p mimic and IRF5 plasmids were co-transfected into fibroblasts
Diversity of interferon antagonist activities mediated by NSP1 proteins of different rotavirus strains
Arnold, Patton
J. Virol. (2011) 85 (5), 1970-9 DOI: 10.1128/JVI.01801-10
Arnold, Patton
J. Virol. (2011) 85 (5), 1970-9 DOI: 10.1128/JVI.01801-10
Cited by 20
AI Summary: OriGene’s pCMV6-XL5-β-TrCP plasmid was used as the template to amplify and construct a FLAG-tagged β-TrCP expression vector, enabling the study of NSP1-mediated degradation of β-TrCP in the investigation of rotavirus interferon antagonist activities.
Snippet: To construct the plasmid expressing FLAG-tagged β-TrCP, the sequence encoding β-TrCP (GenBank accession number NM_033637) was amplified from pCMV6-XL5-β-TrCP (OriGene) by using forward and reverse primers containing unique restriction sites (Table 1). The
Showing 1-3 of 3 papers.
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