PSMB10 (NM_002801) Human Tagged ORF Clone
SKU
RC200437
PSMB10 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10)
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PSMB10 |
| Synonyms | beta2i; LMP10; MECL1; PRAAS5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002801 |
| ORF Size | 819 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002801.4 |
| RefSeq Size | 1009 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 5699 |
| UniProt ID | P40306 |
| Cytogenetics | 16q22.1 |
| Domains | proteasome |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Proteasome |
| MW | 28.8 kDa |
| Summary | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002801" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200437L3 | Lenti ORF clone of Human proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200437L4 | Lenti ORF clone of Human proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10), mGFP tagged | 10 ug |
$600.00
|
|
| RG200437 | PSMB10 (tGFP-tagged) - Human proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10) | 10 ug |
$500.00
|
|
| SC102824 | PSMB10 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10) | 10 ug |
$300.00
|
|
Citations
MLN4924 suppresses kidney renal clear cell carcinoma progression through coordinated inhibition of Cullin1 neddylation and PSMB10-associated NF-κB inflammatory signaling
Liu, Su, Chen et al
Inflamm Res (2026) 75 (1) DOI: 10.1007/s00011-026-02363-x
Liu, Su, Chen et al
Inflamm Res (2026) 75 (1) DOI: 10.1007/s00011-026-02363-x
Cited by 1
PSMB10, the last immunoproteasome gene missing for PRAAS
Sarrabay, Méchin, Salhi et al
J. Allergy Clin. Immunol. (2019)
Sarrabay, Méchin, Salhi et al
J. Allergy Clin. Immunol. (2019)
Cited by 66
AI Summary: OriGene’s pCMV6 vector containing Myc-DDK-tagged wild-type PSMB10 was used as a template to generate and study mutant constructs in the research.
Snippet: A pCMV6 with WT PSMB10 Myc-DDK-tagged in the 3′ end (Origene Technologies, Rockville, Md) was used as a template. The p.Phe14Ser mutant vector was obtained using directed mutagenesis primers (forward: 5′-P-CCCGAGGGGGCTTCTCCTCCGAGAACTGCCAAAG-3′;reverse: 5′-P-CTTTGGCAGTTCTCGGAGGAGAAGCCCCCTCGGG-3′) and high-fidelity Pfu DNA polymerase (Promega, Madison, Wis) following a DpnI digestion. …
Showing 1-2 of 2 papers.
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