DDB2 (NM_000107) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DDB2 |
| Synonyms | DDBB; UV-DDB2; XPE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000107 |
| ORF Size | 1281 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000107.3 |
| RefSeq Size | 1870 bp |
| RefSeq ORF | 1284 bp |
| Locus ID | 1643 |
| UniProt ID | Q92466 |
| Cytogenetics | 11p11.2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Nucleotide excision repair, p53 signaling pathway, Ubiquitin mediated proteolysis |
| MW | 47.9 kDa |
| Summary | This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000107" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200390L1 | Lenti ORF clone of Human damage-specific DNA binding protein 2, 48kDa (DDB2), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200390L2 | Lenti ORF clone of Human damage-specific DNA binding protein 2, 48kDa (DDB2), mGFP tagged | 10 ug |
$757.00
|
|
| RC200390L3 | Lenti ORF clone of Human damage-specific DNA binding protein 2, 48kDa (DDB2), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200390L4 | Lenti ORF clone of Human damage-specific DNA binding protein 2, 48kDa (DDB2), mGFP tagged | 10 ug |
$757.00
|
|
| RG200390 | DDB2 (tGFP-tagged) - Human damage-specific DNA binding protein 2, 48kDa (DDB2) | 10 ug |
$489.00
$657.00
|
|
| SC122547 | DDB2 (untagged)-Human damage-specific DNA binding protein 2, 48kDa (DDB2) | 10 ug |
$457.00
|
|
Citations
Targeted CUL4A inhibition synergizes with cisplatin to yield long-term survival in models of head and neck squamous cell carcinoma through a DDB2-mediated mechanism
Jones, Espitia, Ooi et al
Cell Death Dis (2022) 13 (4), 350 DOI: 10.1038/s41419-022-04798-6
Jones, Espitia, Ooi et al
Cell Death Dis (2022) 13 (4), 350 DOI: 10.1038/s41419-022-04798-6
Cited by 18
AI Summary: OriGene products, specifically the CUL4A cDNA construct (#RC214798L4V) and DDB2 cDNA construct (#RC200390L4V), were used to overexpress these genes in cell models, enabling the authors to investigate their roles in DNA damage response and synergy with cisplatin treatment in head and neck squamous cell carcinoma.
Snippet: e infected with lentiviral particles containing a puromycin resistance gene, a CUL4A (#RC214798L4V) cDNA construct, or a DDB2 (#RC200390L4V) cDNA construct according to the manufacturer’s protocol (Origene, Rockville, MD). …Overexpression efficiency was confirmed by immunoblotti;- a puromycin resistance gene, a CUL4A (#RC214798L4V) cDNA construct, or a DDB2 (#RC200390L4V) cDNA construct according to the manufacturer's protocol (Origene
Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins
Kumar, Theil, Roginskaya et al
Nat Commun (2022) 13 (1), 974 DOI: 10.1038/s41467-022-28642-9
Kumar, Theil, Roginskaya et al
Nat Commun (2022) 13 (1), 974 DOI: 10.1038/s41467-022-28642-9
Cited by 84
AI Summary: OriGene's DDB2-Flag plasmid (RC200390) was used in this study to investigate the role of DDB2 in the repair of 8-oxoguanine DNA lesions, enabling the authors to assess DDB2 recruitment and function at sites of DNA damage.
Snippet: ites of pmNeonGreen-DDB2 plasmid. DDB2(mouse)-mCherry, GFP-DDB1(mouse) and GFP-Cul4A were provided by Dr. Wim Vermeulen67,81. OGG1-GFP was provided by Dr. A. Campalans82. DDB2-Flag was purchased from ORIGENE (RC200390). DDB2(K244E)-Flag mutant was made using the QuickChange II Mutagenesis kit (Agilent, #200523). siRNA transfections 40 nM siRNA was transfected using Lipofectamine 2000 (Thermo Fi
Downregulation of the DNA Repair Gene DDB2 by Arecoline Is through p53's DNA-Binding Domain and Is Correlated with Poor Outcome of Head and Neck Cancer Patients with Betel Quid Consumption
Wang, Huang, Lee et al
Cancers (Basel) (2020) 12 (8) DOI: 10.3390/cancers12082053
Wang, Huang, Lee et al
Cancers (Basel) (2020) 12 (8) DOI: 10.3390/cancers12082053
Cited by 10
AI Summary: OriGene’s DDB2-expressing plasmid (RC200390) was used in this study to restore DDB2 expression and demonstrate its critical role in reversing arecoline-induced inhibition of DNA repair in head and neck cancer cells.
Snippet: The reporter plasmids p21-Luc (2.4-kb CDKN1A/p21Cip1 promoter containing two p53-binding sites) and p3PREc-Luc (3 copies of consensus p53-binding sites and TATA box), as well as the wild-type and mutant p53-expressing plasmids (p53-WT, p53-175m, p53-273m, p53DB-VP16, pGAL4-p53TA, pGAL4-VP16TA), have been described in previous papers [26,38]. The DDB2-expressing plasmid was purchased from OriGene (RC200390, Rockville, MD, USA). …
Showing 1-3 of 3 papers.
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