UXT (NM_004182) Human Tagged ORF Clone

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SKU
RC200343
UXT (Myc-DDK-tagged)-Human ubiquitously-expressed transcript (UXT), transcript variant 2
  $225.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UXT
Synonyms ART-27; STAP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200343 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACGCCCCCTAAGCGGCGGGCGGTGGAGGCCACGGGGGAGAAAGTGCTGCGCTACGAGACCTTCA
TCAGTGACGTGCTGCAGCGGGACTTGCGAAAGGTGCTGGACCATCGAGACAAGGTATATGAGCAGCTGGC
CAAATACCTTCAACTGAGAAATGTCATTGAGCGACTCCAGGAAGCTAAGCACTCGGAGTTATATATGCAG
GTGGATTTGGGCTGTAACTTCTTCGTTGACACAGTGGTCCCAGATACTTCACGCATCTATGTGGCCCTGG
GATATGGTTTTTTCCTGGAGTTGACACTGGCAGAAGCTCTCAAGTTCATTGATCGTAAGAGCTCTCTCCT
CACAGAGCTCAGCAACAGCCTCACCAAGGACTCCATGAATATCAAAGCCCATATCCACATGTTGCTAGAG
GGGCTTAGAGAACTACAAGGCCTGCAGAATTTCCCAGAGAAGCCTCACCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200343 protein sequence
Red=Cloning site Green=Tags(s)

MATPPKRRAVEATGEKVLRYETFISDVLQRDLRKVLDHRDKVYEQLAKYLQLRNVIERLQEAKHSELYMQ
VDLGCNFFVDTVVPDTSRIYVALGYGFFLELTLAEALKFIDRKSSLLTELSNSLTKDSMNIKAHIHMLLE
GLRELQGLQNFPEKPHH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004182
ORF Size 471 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004182.3
RefSeq Size 619 bp
RefSeq ORF 474 bp
Locus ID 8409
UniProt ID Q9UBK9
Cytogenetics Xp11.23
Domains DUF232
Protein Families Druggable Genome
MW 18.2 kDa
Summary The protein encoded by this gene functions as a cofactor that modulates androgen receptor-dependent transcription, and also plays a critical role in tumor necrosis factor-induced apoptosis. Expression of this gene may play a role in tumorigenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Nov 2011
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Documents
Resources
"NM_004182" in other vectors (6)
SKU Description Size Price
RC200343L1 Lenti ORF clone of Human ubiquitously-expressed transcript (UXT), transcript variant 2, Myc-DDK-tagged 10 ug
$525.00
RC200343L2 Lenti ORF clone of Human ubiquitously-expressed transcript (UXT), transcript variant 2, mGFP tagged 10 ug
$525.00
RC200343L3 Lenti ORF clone of Human ubiquitously-expressed transcript (UXT), transcript variant 2, Myc-DDK-tagged 10 ug
$525.00
RC200343L4 Lenti ORF clone of Human ubiquitously-expressed transcript (UXT), transcript variant 2, mGFP tagged 10 ug
$525.00
RG200343 UXT (tGFP-tagged) - Human ubiquitously-expressed transcript (UXT), transcript variant 2 10 ug
$425.00
SC117506 UXT (untagged)-Human ubiquitously-expressed transcript (UXT), transcript variant 2 10 ug
$225.00

Plasmid Map for RC200343

Download sequence for

Cloning scheme for RC200343

Circular map for RC200343

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