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SLC12A4 (NM_005072) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200338
SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $944.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC12A4
Synonyms CTC-479C5.17; hKCC1; KCC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200338 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCACTTCACCGTGGTGCCAGTGGACGGGCCGAGGCGCGGCGACTATGACAACCTCGAGGGGCTCA
GTTGGGTGGACTACGGGGAGCGCGCCGAGCTGGATGACTCGGACGGACATGGCAACCACAGAGAGAGCAG
CCCTTTTCTTTCCCCCTTGGAGGCTTCCAGAGGAATTGACTACTATGACAGGAACCTGGCACTGTTTGAG
GAAGAGCTGGACATCCGCCCAAAGGTATCGTCTCTTCTGGGAAAGCTCGTCAGCTACACCAACCTCACCC
AGGGCGCCAAAGAGCATGAGGAGGCCGAGAGTGGGGAGGGCACCCGCCGGAGGGCAGCCGAGGCACCCAG
CATGGGCACCCTCATGGGGGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTTATCCTCTTCCTGCGG
CTGACCTGGATGGTGGGCACAGCAGGTGTGCTACAGGCCCTCCTCATCGTGCTTATCTGCTGCTGTTGTA
CCCTGCTGACGGCCATCTCCATGAGTGCCATCGCCACCAACGGTGTGGTTCCAGCTGGGGGCTCCTATTT
CATGATCTCTCGTTCACTGGGGCCAGAATTTGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGAACAACA
TTCGCAGCAGCCATGTACATCCTGGGGGCCATCGAGATCTTGCTGACCTACATTGCCCCACCAGCTGCCA
TTTTTTACCCATCGGGTGCTCATGACACGTCGAATGCCACTTTGAACAATATGCGTGTGTATGGGACCAT
TTTCCTGACCTTCATGACCCTGGTGGTGTTTGTGGGGGTCAAGTATGTGAACAAATTTGCCTCGCTCTTC
CTGGCCTGTGTGATCATCTCCATCCTCTCCATCTATGCTGGGGGCATAAAGTCTATATTTGACCCTCCCG
TGTTTCCGGTATGCATGCTGGGCAACAGGACCCTGTCCCGGGACCAGTTTGACATCTGTGCCAAGACAGC
TGTAGTGGACAATGAGACAGTGGCCACCCAGCTATGGAGTTTCTTCTGCCACAGCCCCAACCTTACGACC
GACTCCTGTGACCCCTACTTCATGCTCAACAATGTGACCGAGATCCCTGGCATCCCCGGGGCAGCTGCTG
GTGTGCTCCAGGAAAACCTGTGGAGCGCCTACCTGGAGAAGGGTGACATCGTGGAGAAGCATGGGCTGCC
CTCCGCAGATGCCCCGAGCCTGAAGGAGAGCCTGCCTCTGTACGTGGTCGCTGACATCGCCACATCCTTC
ACCGTGCTGGTCGGCATCTTCTTCCCTTCTGTAACAGGCATCATGGCTGGCTCAAACCGCTCTGGGGACC
TTCGTGACGCCCAGAAGTCTATCCCTGTGGGGACCATTCTGGCCATCATTACAACTTCCCTCGTGTACTT
CAGCAGTGTGGTTCTCTTTGGTGCCTGCATTGAGGGTGTGGTTCTCCGGGACAAGTATGGCGATGGTGTC
AGCAGGAACTTGGTGGTGGGCACACTGGCCTGGCCTTCACCCTGGGTCATCGTCATCGGCTCCTTCTTTT
CAACGTGTGGCGCTGGCCTCCAGAGCCTCACAGGGGCACCACGCCTATTGCAGGCCATTGCCAAGGACAA
CATCATCCCCTTCCTCCGGGTGTTTGGCCACGGGAAGGTGAATGGTGAACCCACATGGGCACTCCTCCTG
ACGGCACTCATCGCCGAGCTGGGCATCCTCATCGCCTCCCTCGACATGGTGGCCCCCATCTTATCCATGT
TCTTTCTGATGTGCTACCTGTTCGTGAACCTCGCCTGTGCGGTGCAGACACTCCTGAGGACCCCCAACTG
GCGGCCCCGGTTCAAGTACTATCACTGGGCGCTGTCCTTCCTGGGCATGAGTCTCTGCCTGGCCCTTATG
TTTGTCTCCTCCTGGTACTATGCCCTGGTGGCCATGCTCATCGCCGGCATGATCTACAAATACATCGAGT
ACCAAGGGGCTGAGAAGGAGTGGGGTGACGGGATCCGAGGCCTGTCCCTGAGCGCTGCCCGCTACGCGCT
GTTGCGGCTGGAGGAGGGGCCTCCTCACACCAAGAACTGGCGGCCGCAGCTGCTGGTGCTGCTGAAGCTG
GACGAGGACCTCCACGTGAAGTACCCGCGGCTCCTCACCTTCGCCTCCCAGCTCAAGGCTGGCAAGGGCC
TGACCATTGTTGGTTCTGTCATCCAGGGGAGCTTCTTGGAGAGCTATGGCGAGGCTCAGGCCGCCGAGCA
GACCATCAAGAACATGATGGAAATTGAGAAGGTGAAGGGCTTCTGCCAGGTGGTGGTGGCCAGCAAGGTG
CGGGAGGGGCTGGCCCACCTCATCCAGTCCTGTGGCCTGGGAGGCATGCGGCATAACTCCGTGGTGCTGG
GCTGGCCCTACGGCTGGCGACAGAGCGAGGACCCCCGTGCCTGGAAGACCTTCATTGACACCGTGCGCTG
CACTACGGCTGCCCACCTGGCCCTGCTCGTGCCCAAGAACATCGCCTTCTACCCCAGCAACCACGAGCGC
TACCTGGAGGGCCACATAGACGTGTGGTGGATCGTGCACGATGGTGGCATGCTCATGCTTCTGCCCTTCC
TGCTGCGCCAGCATAAGGTCTGGAGGAAGTGCCGGATGCGCATCTTCACAGTGGCCCAGATGGATGACAA
CAGCATCCAGATGAAGAAGGACCTGGCTGTCTTTCTGTACCATCTGCGCCTTGAGGCCGAGGTGGAGGTG
GTGGAGATGCATAACAGTGACATCTCTGCATACACCTACGAGCGGACGCTGATGATGGAGCAGCGGTCGC
AGATGCTGCGGCAGATGAGACTGACCAAGACTGAGCGGGAGCGAGAAGCCCAGCTGGTCAAGGATCGGCA
CTCGGCCCTGAGGCTGGAGAGCCTGTACTCGGACGAGGAAGATGAGTCTGCAGTGGGGGCTGACAAGATC
CAGATGACGTGGACCAGGGACAAGTACATGACTGAGACCTGGGACCCCAGCCATGCCCCTGACAATTTCC
GGGAGCTGGTGCACATTAAGCCGGACCAATCCAATGTGCGGCGCATGCACACTGCTGTGAAGCTCAATGA
AGTCATTGTCACGCGCTCCCACGACGCCCGCCTGGTTCTCCTAAACATGCCTGGCCCACCCAGGAACAGT
GAGGGCGACGAGAACTACATGGAGTTCCTCGAGGTGCTGACCGAGGGCCTTGAGCGGGTGCTGTTGGTGC
GCGGTGGTGGCCGTGAAGTCATCACCATCTACTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200338 protein sequence
Red=Cloning site Green=Tags(s)

MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAELDDSDGHGNHRESSPFLSPLEASRGIDYYDRNLALFE
EELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIFGVILFLR
LTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTT
FAAAMYILGAIEILLTYIAPPAAIFYPSGAHDTSNATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLF
LACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTAVVDNETVATQLWSFFCHSPNLTT
DSCDPYFMLNNVTEIPGIPGAAAGVLQENLWSAYLEKGDIVEKHGLPSADAPSLKESLPLYVVADIATSF
TVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVVLRDKYGDGV
SRNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKVNGEPTWALLL
TALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALM
FVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKL
DEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKV
REGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHER
YLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAVFLYHLRLEAEVEV
VEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKI
QMTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPRNS
EGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005072
ORF Size 3255 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005072.3, NP_005063.1
RefSeq Size 4773 bp
RefSeq ORF 3258 bp
Locus ID 6560
UniProt ID Q9UP95
Cytogenetics 16q22.1
Domains KCl_Cotrans_1
Protein Families Transmembrane
MW 120.6 kDa
Summary This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Jan 2013
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Documents
Resources
"NM_005072" in other vectors (4)
SKU Description Size Price
RC200338L3 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1, Myc-DDK-tagged 10 ug
$1,244.00
RC200338L4 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1, mGFP tagged 10 ug
$1,244.00
RG200338 SLC12A4 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1 10 ug
$789.00 $1,144.00
SC320286 SLC12A4 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1 10 ug
$945.00

Plasmid Map for RC200338

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200338
Circular map for RC200338

file-alt Citations

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