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SLC12A4 (NM_005072) Human Untagged Clone

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SKU
SC320286
SLC12A4 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1
  $945.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SLC12A4
Synonyms CTC-479C5.17; hKCC1; KCC1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_005072.3 GGCGGGGACAGCGGCGGGGACAGCGGCGGGCGGCTGGGACGGCGGGTGCGGCGGGGCCGA
GCCCGCACGATGCCTCACTTCACCGTGGTGCCAGTGGACGGGCCGAGGCGCGGCGACTAT
GACAACCTCGAGGGGCTCAGTTGGGTGGACTACGGGGAGCGCGCCGAGCTGGATGACTCG
GACGGACATGGCAACCACAGAGAGAGCAGCCCTTTTCTTTCCCCCTTGGAGGCTTCCAGA
GGAATTGACTACTATGACAGGAACCTGGCACTGTTTGAGGAAGAGCTGGACATCCGCCCA
AAGGTATCGTCTCTTCTGGGAAAGCTCGTCAGCTACACCAACCTCACCCAGGGCGCCAAA
GAGCATGAGGAGGCCGAGAGTGGGGAGGGCACCCGCCGGAGGGCAGCCGAGGCACCCAGC
ATGGGCACCCTCATGGGGGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTTATCCTC
TTCCTGCGGCTGACCTGGATGGTGGGCACAGCAGGTGTGCTACAGGCCCTCCTCATCGTG
CTTATCTGCTGCTGTTGTACCCTGCTGACGGCCATCTCCATGAGTGCCATCGCCACCAAC
GGTGTGGTTCCAGCTGGGGGCTCCTATTTCATGATCTCTCGTTCACTGGGGCCAGAATTT
GGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGAACAACATTCGCAGCAGCCATGTACATC
CTGGGGGCCATCGAGATCTTGCTGACCTACATTGCCCCACCAGCTGCCATTTTTTACCCA
TCGGGTGCTCATGACACGTCGAATGCCACTTTGAACAATATGCGTGTGTATGGGACCATT
TTCCTGACCTTCATGACCCTGGTGGTGTTTGTGGGGGTCAAGTATGTGAACAAATTTGCC
TCGCTCTTCCTGGCCTGTGTGATCATCTCCATCCTCTCCATCTATGCTGGGGGCATAAAG
TCTATATTTGACCCTCCCGTGTTTCCGGTATGCATGCTGGGCAACAGGACCCTGTCCCGG
GACCAGTTTGACATCTGTGCCAAGACAGCTGTAGTGGACAATGAGACAGTGGCCACCCAG
CTATGGAGTTTCTTCTGCCACAGCCCCAACCTTACGACCGACTCCTGTGACCCCTACTTC
ATGCTCAACAATGTGACCGAGATCCCTGGCATCCCCGGGGCAGCTGCTGGTGTGCTCCAG
GAAAACCTGTGGAGCGCCTACCTGGAGAAGGGTGACATCGTGGAGAAGCATGGGCTGCCC
TCCGCAGATGCCCCGAGCCTGAAGGAGAGCCTGCCTCTGTACGTGGTCGCTGACATCGCC
ACATCCTTCACCGTGCTGGTCGGCATCTTCTTCCCTTCTGTAACAGGCATCATGGCTGGC
TCAAACCGCTCTGGGGACCTTCGTGACGCCCAGAAGTCTATCCCTGTGGGGACCATTCTG
GCCATCATTACAACTTCCCTCGTGTACTTCAGCAGTGTGGTTCTCTTTGGTGCCTGCATT
GAGGGTGTGGTTCTCCGGGACAAGTATGGCGATGGTGTCAGCAGGAACTTGGTGGTGGGC
ACACTGGCCTGGCCTTCACCCTGGGTCATCGTCATCGGCTCCTTCTTTTCAACGTGTGGC
GCTGGCCTCCAGAGCCTCACAGGGGCACCACGCCTATTGCAGGCCATTGCCAAGGACAAC
ATCATCCCCTTCCTCCGGGTGTTTGGCCACGGGAAGGTGAATGGTGAACCCACATGGGCA
CTCCTCCTGACGGCACTCATCGCCGAGCTGGGCATCCTCATCGCCTCCCTCGACATGGTG
GCCCCCATCTTATCCATGTTCTTTCTGATGTGCTACCTGTTCGTGAACCTCGCCTGTGCG
GTGCAGACACTCCTGAGGACCCCCAACTGGCGGCCCCGGTTCAAGTACTATCACTGGGCG
CTGTCCTTCCTGGGCATGAGTCTCTGCCTGGCCCTTATGTTTGTCTCCTCCTGGTACTAT
GCCCTGGTGGCCATGCTCATCGCCGGCATGATCTACAAATACATCGAGTACCAAGGGGCT
GAGAAGGAGTGGGGTGACGGGATCCGAGGCCTGTCCCTGAGCGCTGCCCGCTACGCGCTG
TTGCGGCTGGAGGAGGGGCCTCCTCACACCAAGAACTGGCGGCCGCAGCTGCTGGTGCTG
CTGAAGCTGGACGAGGACCTCCACGTGAAGTACCCGCGGCTCCTCACCTTCGCCTCCCAG
CTCAAGGCTGGCAAGGGCCTGACCATTGTTGGTTCTGTCATCCAGGGGAGCTTCTTGGAG
AGCTATGGCGAGGCTCAGGCCGCCGAGCAGACCATCAAGAACATGATGGAAATTGAGAAG
GTGAAGGGCTTCTGCCAGGTGGTGGTGGCCAGCAAGGTGCGGGAGGGGCTGGCCCACCTC
ATCCAGTCCTGTGGCCTGGGAGGCATGCGGCATAACTCCGTGGTGCTGGGCTGGCCCTAC
GGCTGGCGACAGAGCGAGGACCCCCGTGCCTGGAAGACCTTCATTGACACCGTGCGCTGC
ACTACGGCTGCCCACCTGGCCCTGCTCGTGCCCAAGAACATCGCCTTCTACCCCAGCAAC
CACGAGCGCTACCTGGAGGGCCACATAGACGTGTGGTGGATCGTGCACGATGGTGGCATG
CTCATGCTTCTGCCCTTCCTGCTGCGCCAGCATAAGGTCTGGAGGAAGTGCCGGATGCGC
ATCTTCACAGTGGCCCAGATGGATGACAACAGCATCCAGATGAAGAAGGACCTGGCTGTC
TTTCTGTACCATCTGCGCCTTGAGGCCGAGGTGGAGGTGGTGGAGATGCATAACAGTGAC
ATCTCTGCATACACCTACGAGCGGACGCTGATGATGGAGCAGCGGTCGCAGATGCTGCGG
CAGATGAGACTGACCAAGACTGAGCGGGAGCGAGAAGCCCAGCTGGTCAAGGATCGGCAC
TCGGCCCTGAGGCTGGAGAGCCTGTACTCGGACGAGGAAGATGAGTCTGCAGTGGGGGCT
GACAAGATCCAGATGACGTGGACCAGGGACAAGTACATGACTGAGACCTGGGACCCCAGC
CATGCCCCTGACAATTTCCGGGAGCTGGTGCACATTAAGCCGGACCAATCCAATGTGCGG
CGCATGCACACTGCTGTGAAGCTCAATGAAGTCATTGTCACGCGCTCCCACGACGCCCGC
CTGGTTCTCCTAAACATGCCTGGCCCACCCAGGAACAGTGAGGGCGACGAGAACTACATG
GAGTTCCTCGAGGTGCTGACCGAGGGCCTTGAGCGGGTGCTGTTGGTGCGCGGTGGTGGC
CGTGAAGTCATCACCATCTACTCCTGAGCCCAGTGTCATCTTGTGGCCTGGAGTCGAGGT
CTTGGCCAGGACATAACAAGCTGTGGTCTGGGGTAACAGCCTCTTCCCAGCACCCACCTG
CCAGCCCTGCTTGCCTGGCCCTGTCCTGGACCCAGCTTTGCTAGGTCTCCTTGGAAACCA
GGCCTGGGCCTCAAAATGGAGATGGATCCCAGGTCTTGTGGGACCCTGGGATGTTTGGGG
ACTTTACTATCTAGCACCCCAGTAGGCCTGTCCTGGCCAGAGAAGACTGGTAGGGGCCGA
GTGGGGTTTGAAGGCAGCCGGCCCGGCCCAGCCCAGGAGCGCTATTTATTGCATATTTAT
TGTTTGGATGTCACCATCAGAGACGAAGGGAAGGGTAGCCAGGGAGGGAGTCCAGCCCAG
CTGCCTGCAGGAAGATCTGGCTCAGTCTACTATGGGCAGGGCCCCCCACCAAGCTGAGCC
GAATGGAGACAGCTGAGCTGAGGCCTGACTTTTTCAATAAAACATTGTGTAGTTCTGGGC
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_005072
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005072.3, NP_005063.1
RefSeq Size 3884 bp
RefSeq ORF 3258 bp
Locus ID 6560
UniProt ID Q9UP95
Cytogenetics 16q22.1
Domains KCl_Cotrans_1
Protein Families Transmembrane
Summary This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Jan 2013
Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a.
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Documents
Resources
"NM_005072" in other vectors (4)
SKU Description Size Price
RC200338 SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1 10 ug
$589.00 $944.00
RC200338L3 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1, Myc-DDK-tagged 10 ug
$1,244.00
RC200338L4 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1, mGFP tagged 10 ug
$1,244.00
RG200338 SLC12A4 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1 10 ug
$789.00 $1,144.00

file-alt Citations

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