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ABCF2 (NM_007189) Human Tagged ORF Clone

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SKU
RC200323
ABCF2 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $580.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ABCF2
Synonyms ABC28; EST133090; HUSSY-18; HUSSY18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200323 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTCCGACCTGGCCAAGAAGAAGGCAGCCAAAAAGAAGGAGGCTGCCAAAGCTCGACAGCGGCCCA
GAAAAGGACATGAAGAAAATGGAGATGTTGTCACAGAACCACAGGTGGCAGAGAAGAATGAGGCCAATGG
CAGAGAGACCACAGAAGTAGATTTGCTGACCAAGGAGCTAGAGGACTTTGAGATGAAGAAAGCTGCTGCT
CGAGCTGTCACTGGCGTCCTGGCCTCTCACCCCAACAGTACTGATGTTCACATCATCAACCTCTCACTCA
CCTTTCATGGTCAAGAGCTGCTCAGTGACACCAAACTGGAATTAAACTCAGGCCGTCGTTATGGCCTCAT
TGGTTTAAATGGAATTGGAAAGTCCATGCTGCTCTCTGCTATTGGGAAGCGTGAAGTGCCCATCCCTGAG
CACATCGACATCTACCATCTGACTCGAGAGATGCCCCCTAGTGACAAGACACCCTTGCATTGTGTGATGG
AAGTCGACACAGAGCGGGCCATGCTGGAGAAAGAGGCAGAGCGGCTGGCTCATGAGGATGCGGAGTGTGA
GAAGCTCATGGAGCTCTACGAGCGCCTGGAGGAGCTGGATGCCGACAAGGCAGAGATGAGGGCCTCGCGG
ATCTTGCATGGACTGGGTTTCACACCTGCCATGCAGCGCAAGAAGCTAAAAGACTTCAGTGGGGGCTGGA
GGATGAGGGTTGCCCTTGCCAGAGCCCTCTTTATTCGGCCCTTCATGCTGCTCCTGGATGAGCCTACCAA
CCACCTGGACCTAGATGCTTGCGTGTGGTTGGAAGAAGAACTAAAAACTTTTAAGCGCATCTTGGTCCTC
GTCTCCCATTCCCAGGATTTTCTGAATGGTGTCTGTACCAATATCATTCACATGCACAACAAGAAACTGA
AGTATTATACGGGTAATTATGATCAGTATGTGAAGACGCGGCTAGAGCTGGAGGAGAACCAGATGAAGAG
GTTTCACTGGGAGCAAGATCAGATTGCACACATGAAGAACTACATTGCGAGGTTTGGTCATGGCAGTGCC
AAGCTGGCCCGGCAGGCCCAGAGCAAGGAGAAGACGCTACAGAAAATGATGGCATCAGGACTGACAGAGA
GGGTCGTGAGCGATAAGACACTGTCATTTTATTTCCCACCATGTGGCAAGATCCCTCCACCTGTCATTAT
GGTGCAAAATGTGAGCTTCAAGTATACAAAAGATGGGCCTTGCATCTACAATAATCTAGAATTTGGAATT
GACCTTGACACACGAGTGGCTCTGGTAGGGCCCAATGGAGCAGGGAAGTCAACTCTTCTGAAGCTGCTAA
CTGGAGAGCTACTACCCACAGATGGCATGATCCGAAAACACTCTCATGTCAAGATAGGGCGTTACCATCA
GCATTTACAAGAGCAGCTGGACTTAGATCTCTCACCTTTGGAGTACATGATGAAGTGCTACCCAGAGATC
AAGGAGAAGGAAGAAATGAGGAAGATCATTGGGCGATACGGTCTCACTGGGAAACAACAGGTGAGCCCAA
TCCGGAACTTGTCAGACGGGCAGAAGTGCCGAGTGTGTCTGGCCTGGCTGGCCTGGCAGAACCCCCACAT
GCTCTTCCTGGATGAACCCACCAATCACCTGGATATCGAGACCATCGACGCCCTGGCAGATGCCATCAAT
GAGTTTGAGGGTGGTATGATGCTGGTCAGCCATGACTTCAGACTCATTCAGCAGGTTGCACAGGAAATTT
GGGTCTGTGAGAAGCAGACAATCACCAAGTGGCCTGGAGACATCCTGGCTTACAAGGAGCACCTCAAGTC
CAAGCTGGTGGATGAGGAGCCCCAGCTCACCAAGAGGACCCACAACGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200323 protein sequence
Red=Cloning site Green=Tags(s)

MPSDLAKKKAAKKKEAAKARQRPRKGHEENGDVVTEPQVAEKNEANGRETTEVDLLTKELEDFEMKKAAA
RAVTGVLASHPNSTDVHIINLSLTFHGQELLSDTKLELNSGRRYGLIGLNGIGKSMLLSAIGKREVPIPE
HIDIYHLTREMPPSDKTPLHCVMEVDTERAMLEKEAERLAHEDAECEKLMELYERLEELDADKAEMRASR
ILHGLGFTPAMQRKKLKDFSGGWRMRVALARALFIRPFMLLLDEPTNHLDLDACVWLEEELKTFKRILVL
VSHSQDFLNGVCTNIIHMHNKKLKYYTGNYDQYVKTRLELEENQMKRFHWEQDQIAHMKNYIARFGHGSA
KLARQAQSKEKTLQKMMASGLTERVVSDKTLSFYFPPCGKIPPPVIMVQNVSFKYTKDGPCIYNNLEFGI
DLDTRVALVGPNGAGKSTLLKLLTGELLPTDGMIRKHSHVKIGRYHQHLQEQLDLDLSPLEYMMKCYPEI
KEKEEMRKIIGRYGLTGKQQVSPIRNLSDGQKCRVCLAWLAWQNPHMLFLDEPTNHLDIETIDALADAIN
EFEGGMMLVSHDFRLIQQVAQEIWVCEKQTITKWPGDILAYKEHLKSKLVDEEPQLTKRTHNV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007189
ORF Size 1869 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007189.3
RefSeq Size 4696 bp
RefSeq ORF 1872 bp
Locus ID 10061
UniProt ID Q9UG63, A0A090N7X1
Cytogenetics 7q36.1
Protein Families Druggable Genome
MW 71.3 kDa
Summary This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Related pseudogenes have been identified on chromosomes 3 and 7. provided by RefSeq, Mar 2019
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Documents
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"NM_007189" in other vectors (6)
SKU Description Size Price
RC200323L1 Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$880.00
RC200323L2 Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$880.00
RC200323L3 Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$880.00
RC200323L4 Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$880.00
RG200323 ABCF2 (tGFP-tagged) - Human ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00 $780.00
SC320248 ABCF2 (untagged)-Human ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$581.00

Plasmid Map for RC200323

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200323
Circular map for RC200323

file-alt Citations

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