OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

TRAFD1 (NM_006700) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC200265
TRAFD1 (Myc-DDK-tagged)-Human TRAF-type zinc finger domain containing 1 (TRAFD1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $542.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA809366 TRAFD1 mouse monoclonal antibody,clone OTI3E8 100 ul
$447.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "TRAFD1 or NM_006700"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRAFD1
Synonyms FLN29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200265 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAATTTCTAGATGACCAGGAAACTCGACTGTGTGACAACTGCAAAAAAGAAATTCCTGTGTTTA
ACTTTACCATCCATGAGATCCACTGTCAAAGGAACATTGGTATGTGTCCTACCTGTAAGGAACCATTTCC
CAAATCTGACATGGAGACTCACATGGCTGCAGAACACTGTCAGGTGACCTGCAAATGTAACAAGAAGTTG
GAGAAGAGGCTGTTAAAGAAGCATGAGGAGACTGAGTGCCCTTTGCGGCTTGCTGTCTGCCAGCACTGTG
ATTTAGAACTTTCCATTCTCAAACTGAAGGAACATGAAGATTATTGTGGTGCCCGGACGGAACTATGTGG
CAACTGTGGTCGCAATGTCCTTGTGAAAGATCTGAAGACTCACCCTGAAGTTTGTGGGAGAGAGGGGGAG
GAAAAGAGAAATGAGGTTGCCATACCTCCTAATGCATATGATGAATCTTGGGGTCAGGATGGAATCTGGA
TTGCATCCCAACTCCTCAGACAAATTGAGGCTCTGGACCCACCCATGAGGCTGCCGCGAAGGCCCCTGAG
AGCCTTTGAATCAGATGTTTTCCACAATAGAACTACCAACCAAAGGAACATTACAGCCCAGGTTTCAATT
CAGAATAATCTGTTTGAAGAACAAGAGAGGCAGGAAAGGAATAGAGGCCAACAGCCCCCCAAAGAGGGTG
GTGAAGAGAGTGCAAACTTGGACTTCATGTTGGCCCTAAGTCTGCAAAATGAAGGCCAAGCCTCCAGTGT
GGCAGAGCAGGACTTCTGGAGGGCCGTATGTGAGGCCGACCAGTCTCATGGCGGTCCCAGGTCTCTCAGT
GACATAAAGGGTGCAGCTGACGAGATCATGTTGCCTTGTGAATTTTGTGAGGAGCTCTACCCAGAGGAAC
TGCTGATTGACCATCAGACAAGCTGTAACCCTTCACGTGCCTTACCTTCACTCAATACTGGCAGCTCTTC
CCCCAGAGGGGTGGAGGAACCTGATGTCATCTTCCAGAACTTCTTGCAACAGGCTGCAAGTAACCAGTTA
GACTCTTTGATGGGCCTGAGCAATTCACACCCTGTGGAGGAGAGCATCATTATCCCATGTGAATTCTGTG
GGGTACAGCTGGAAGAGGAGGTGCTGTTCCATCACCAGGACCAGTGTGACCAACGCCCAGCCACTGCAAC
CAACCATGTGACAGAGGGGATTCCTAGACTGGATTCCCAGCCTCAAGAGACCTCACCAGAGCTGCCCAGG
AGGCGTGTCAGACACCAGGGAGACCTGTCTTCTGGTTACCTGGATGATACTAAGCAGGAAACAGCTAATG
GGCCCACCTCCTGTCTGCCTCCCAGCCGACCCATTAACAATATGACAGCTACCTATAACCAGCTATCGAG
ATCAACATCAGGCCCCAGACCTGGGTGCCAGCCCAGCTCTCCTTGTGTGCCGAAGCTCAGCAACTCAGAC
AGCCAGGACATCCAGGGGCGGAATCGAGACAGCCAGAATGGGGCCATAGCCCCTGGGCACGTTTCAGTGA
TTCGCCCTCCTCAAAATCTCTACCCAGAAAACATTGTGCCCTCTTTCTCCCCTGGGCCTTCAGGGAGATA
CGGAGCTAGTGGTAGGAGTGAAGGTGGCAGGAATTCCCGGGTCACCCCTGCAGCTGCCAACTACCGCAGC
AGAACTGCAAAGGCAAAGCCTTCCAAGCAACAGGGAGCTGGGGATGCAGAAGAGGAAGAGGAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200265 protein sequence
Red=Cloning site Green=Tags(s)

MAEFLDDQETRLCDNCKKEIPVFNFTIHEIHCQRNIGMCPTCKEPFPKSDMETHMAAEHCQVTCKCNKKL
EKRLLKKHEETECPLRLAVCQHCDLELSILKLKEHEDYCGARTELCGNCGRNVLVKDLKTHPEVCGREGE
EKRNEVAIPPNAYDESWGQDGIWIASQLLRQIEALDPPMRLPRRPLRAFESDVFHNRTTNQRNITAQVSI
QNNLFEEQERQERNRGQQPPKEGGEESANLDFMLALSLQNEGQASSVAEQDFWRAVCEADQSHGGPRSLS
DIKGAADEIMLPCEFCEELYPEELLIDHQTSCNPSRALPSLNTGSSSPRGVEEPDVIFQNFLQQAASNQL
DSLMGLSNSHPVEESIIIPCEFCGVQLEEEVLFHHQDQCDQRPATATNHVTEGIPRLDSQPQETSPELPR
RRVRHQGDLSSGYLDDTKQETANGPTSCLPPSRPINNMTATYNQLSRSTSGPRPGCQPSSPCVPKLSNSD
SQDIQGRNRDSQNGAIAPGHVSVIRPPQNLYPENIVPSFSPGPSGRYGASGRSEGGRNSRVTPAAANYRS
RTAKAKPSKQQGAGDAEEEEEE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006700
ORF Size 1746 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006700.3
RefSeq Size 2665 bp
RefSeq ORF 1749 bp
Locus ID 10906
UniProt ID O14545, A0A024RBK4
Cytogenetics 12q24.13
Domains zf-C2H2, zf-TRAF
MW 64.8 kDa
Summary The innate immune system confers host defense against viral and microbial infection, and TRAFD1 is a negative feedback regulator that controls excessive immune responses (Sanada et al., 2008 PubMed 18849341).supplied by OMIM, Dec 2009
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_006700" in other vectors (6)
SKU Description Size Price
RC200265L1 Lenti ORF clone of Human TRAF-type zinc finger domain containing 1 (TRAFD1), transcript variant 2, Myc-DDK-tagged 10 ug
$842.00
RC200265L2 Lenti ORF clone of Human TRAF-type zinc finger domain containing 1 (TRAFD1), transcript variant 2, mGFP tagged 10 ug
$842.00
RC200265L3 Lenti ORF clone of Human TRAF-type zinc finger domain containing 1 (TRAFD1), transcript variant 2, Myc-DDK-tagged 10 ug
$842.00
RC200265L4 Lenti ORF clone of Human TRAF-type zinc finger domain containing 1 (TRAFD1), transcript variant 2, mGFP tagged 10 ug
$842.00
RG200265 TRAFD1 (tGFP-tagged) - Human TRAF-type zinc finger domain containing 1 (TRAFD1), transcript variant 2 10 ug
$489.00 $742.00
SC321333 TRAFD1 (untagged)-Human TRAF-type zinc finger domain containing 1 (TRAFD1), transcript variant 2 10 ug
$543.00

Plasmid Map for RC200265

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200265
Circular map for RC200265

file-alt Citations

Latent Membrane Protein 1 of Epstein-Barr Virus Promotes RIG-I Degradation Mediated by Proteasome Pathway
Xu, Sun, Liu et al
Front Immunol (2018) 9, 1446 DOI: 10.3389/fimmu.2018.01446
AI Summary: OriGene products, specifically the TRAFD1 (RC200265) and CHIP (RC200310) plasmids, were used in this study to investigate the molecular mechanisms by which EBV's LMP1 protein promotes RIG-I degradation via the proteasome pathway.
Snippet: The promoter luciferase reporter plasmids IFN-β-Luc, NF-kB-Luc, IFN-stimulated response element (ISRE)-Luc, and expression plasmids RIG-I, RIG-IN, MDA5C, MAVS, TBK1, IRF3/5D were provided by Liu W (22) (Chinese Academy of Sciences, China). Plasmids TRAFD1 (RC200265) and CHIP (RC200310) were products of OriGene company
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products