HEY1 (NM_012258) Human Tagged ORF Clone
SKU
RC200257
HEY1 (Myc-DDK-tagged)-Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HEY1 |
| Synonyms | BHLHb31; CHF2; HERP2; HESR1; hHRT1; HRT-1; NERP2; OAF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012258 |
| ORF Size | 912 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012258.4 |
| RefSeq Size | 2319 bp |
| RefSeq ORF | 915 bp |
| Locus ID | 23462 |
| UniProt ID | Q9Y5J3 |
| Cytogenetics | 8q21.13 |
| Domains | HLH, ORANGE |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 32.6 kDa |
| Summary | This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012258" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200257L1 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200257L2 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC200257L3 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200257L4 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RG200257 | HEY1 (tGFP-tagged) - Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1 | 10 ug |
$650.00
|
|
| SC115467 | HEY1 (untagged)-Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Genomic profiling identifies genes and pathways dysregulated by HEY1-NCOA2 fusion and shines a light on mesenchymal chondrosarcoma tumorigenesis
Qi, Rosikiewicz, Yin et al
J Pathol (2022) 257 (5), 579-592 DOI: 10.1002/path.5899
Qi, Rosikiewicz, Yin et al
J Pathol (2022) 257 (5), 579-592 DOI: 10.1002/path.5899
Cited by 25
AI Summary: In this study, OriGene products including the HEY1 cDNA clone (RC209417), NCOA2 cDNA clone (RC211238), and anti-FLAG antibody (TA50011-100) were used to generate and detect expression of HEY1, NCOA2, and HEY1-NCOA2 fusion proteins in iPSC-derived mesenchymal stem cells, enabling functional analysis of the fusion’s role in mesenchymal chondrosarcoma.
Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression
Tsuru, Setoguchi, Matsunoshita et al
Br. J. Cancer (2015) 112 Suppl, 1232-40 DOI: 10.1038/bjc.2015.84
Tsuru, Setoguchi, Matsunoshita et al
Br. J. Cancer (2015) 112 Suppl, 1232-40 DOI: 10.1038/bjc.2015.84
Cited by 40
Showing 1-2 of 2 papers.
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