HEY1 (NM_012258) Human Untagged Clone

SKU SC115467

HEY1 (untagged)-Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: HEY1
• Synonyms: BHLHb31; CHF2; HERP2; HESR1; hHRT1; HRT-1; NERP2; OAF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012258 edited; GAATTCGGCACGAGGCAAAGCGTGAGCGGGATCAGTGTGTGCGGAACGCAAGCAGCCGAG; AGCGGAGAGGCGCCGCTGTAGTTAACTCCTCCCTGCCCGCCGCGCCGACCCTCCCCAGGA; ACCCCCAGGGAGCCAGCATGAAGCGAGCTCACCCCGAGTACAGCTCCTCGGACAGCGAGC; TGGACGAGACCATCGAGGTGGAGAAGGAGAGTGCGGACGAGAATGGAAACTTGAGTTCGG; CTCTAGGTTCCATGTCCCCAACTACATCTTCCCAGATTTTGGCCAGAAAAAGACGGAGAG; GAATAATTGAGAAGCGCCGACGAGACCGGATCAATAACAGTTTGTCTGAGCTGAGAAGGC; TGGTACCCAGTGCTTTTGAGAAGCAGGGATCTGCTAAGCTAGAAAAAGCCGAGATCCTGC; AGATGACCGTGGATCACCTGAAAATGCTGCATACGGCAGGAGGGAAAGGTTACTTTGACG; CGCACGCCCTTGCTATGGACTATCGGAGTTTGGGATTTCGGGAATGCCTGGCAGAAGTTG; CGCGTTATCTGAGCATCATTGAAGGACTAGATGCCTCTGACCCGCTTCGAGTTCGACTGG; TTTCGCATCTCAACAACTACGCTTCCCAGCGGGAAGCCGCGAGCGGCGCCCACGCGGGCC; TCGGACACATTCCCTGGGGGACCGTCTTCGGACATCACCCGCACATCGCGCACCCGCTGT; TGCTGCCCCAGAACGGCCACGGGAACGCGGGCACCACGGCCTCACCCACGGAACCGCACC; ACCAGGGCAGGCTGGGCTCGGCACATCCGGAGGCGCCTGCTTTGCGAGCGCCCCCTAGCG; GCAGCCTCGGACCGGTGCTCCCTGTGGTCACCTCCGCCTCCAAACTGTCGCCGCCTCTGC; TCTCCTCAGTGGCCTCCCTGTCGGCCTTCCCCTTCTCTTTCGGCTCCTTCCACTTACTGT; ATCCCAATGCACTGAGCCCTTCAGCACCCACGCAGGCTGCAAACCTTGGCAAGCCCTATA; GACCTTGGGGGACGGAGATCGGAGCTTTTTAAAGAACTGATGTAGAATGAGGGAGGGGAA; AGTTTAAAATCCCAGCTGGGCTGGACTGTTGCCAACATCACCTTAAAGTCGTCAGTAAAA; GTAAAAAGGAAAAAGGTACACTTTCAGATAATTTTTTTTTTAAAGACTAAAGGTTTGTTG; GTTTACTTTTATCTTTTTTAATGTTTTTTTCATCATGTCATGTATTAGCAGTTTTTAAAA; ACTAGTTGTTAAATTTTGTTCAAGACATTAAATTGAAATAGTGAGTATAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012258 unedited; GCACGAGGCAAAGCGTGAGCGGGATCAGTGTGTGCGGAACGCAAGCAGCCGAGAGCGGAG; AGGCGCCGCTGTAGTTAACTCCTCCCTGCCCGCCGCGCCGACCCTCCCCAGGAACCCCCA; GGGAGCCAGCATGAAGCGAGCTCACCCCGAGTACAGCTCCTCGGACAGCGAGCTGGACGA; GACCATCGAGGTGGAGAAGGAGAGTGCGGACGAGAATGGAAACTTGAGTTCGGCTCTAGG; TTCCATGTCCCCAACTACATCTTCCCAGATTTTGGCCAGAAAAAGACGGAGAGGAATAAT; TGAGAAGCGCCGACGAGACCGGATCAATAACAGTTTGTCTGAGCTGAGAAGGCTGGTACC; CAGTGCTTTTGAGAAGCAGGGATCTGCTAAGCTAGAAAAAGCCGAGATCCTGCAGATGAC; CGTGGATCACCTGAAAATGCTGCATACGGCAGGAGGGAAAGGTTACTTTGACGCGCACGC; CCTTGCTATGGACTATCGGAGTTTGGGATTTCGGGAATGCCTGGCAGAAGTTGCGCGTTA; TCTGAGCATCATTGAAGGACTAGATGCCTCTGACCCGCTTCGAGTTCGACTGGTTTCGCA; TCTCAACAACTACGCTTCCCAGCGGGAAGCCGCGAGCGGCGCCCACGCGGGCCTCGGACA; CATTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012258 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACTCAC; TATTTCAATTTAATGTCTTGAACAAAATTTAACAACTAGTTTTTAAAAACTGCTAATACA; TGACATGATGAAAAAAACATTAAAAAAGATAAAAGTAAACCAACAAACCTTTAGTCTTTA; AAAAAAAAATTATCTGAAAGGGTACCTTTTTCCTTTTTACTTTTACTGACGACTTTAAGG; GGATGTTGGCAACAGTCCAGCCCAGCTGGGATTTTAAACTTTCCCCTCCCTCATTCTACA; TCAGTTCTTTAAAAAGCTCCGATCTCCGTCCCCCAAGGTCTATAGGGCTTGCCAAGGTTT; GCAGCCTGCGTGGGTGCTGAAGGGCTCATTGCATTGGGATACAGTAAGTGGAAGGAGCCG; AAAGAAAAGGGGAAGGCCGACAGGGAGGCCACTGAGGAGAGCAAAGGCGGCGACAGTTTG; GAGGCGGAGGTGACCACAGGGAGCACCGGTCCGAGGCTGCCGCTAGGGGGCGCTCGCAAA; GCAGGCGCCTCCGGATGTGCCGAGCCCAGCCTGCCCTGGTGGTGCGGTTCCGTGGGTGAT; GCCGTGGTGCCCGCGTTCCCGTGGCCGTTCTGGGGCATCAACAGCGGGTGCGCGATGTGC; GGGTGATGTCCGAATACAGTCCCCCATGTAATGTGTCCGAAGCCCGCGTGGCCGCCGCTC; GCAGCTTTCCGCTGGGAAGCGTTATTGTTGAGATGCGAAACCAGCGAACTCGAACCGGCA; AAAGCCATCAGCCCCTCAAGGATGCTCAGATACGCGCACTTTTTGCCAGCATTCCCGAAT; CCCAACTCCGATAGCCCTACCAGGGCGGGCCCGCAAATACCTTTTCTTCTCGCGTAGCAG; ATTTTAGGGATCCAGGCCTCGGAGGACTGGGTTTTTTACTTACAAAACCGGCTTCTAACC; AG;
• Restriction Sites: NotI-NotI
• ACCN: NM_012258
• Insert Size: 1310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012258.2, NP_036390.2
• RefSeq Size: 2223 bp
• RefSeq ORF: 915 bp
• Locus ID: 23462
• UniProt ID: Q9Y5J3
• Cytogenetics: 8q21.13
• Domains: HLH, ORANGE
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the predominant isoform (a).