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POMGNT1 (NM_017739) Human Tagged ORF Clone

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SKU
RC200176
POMGNT1 (Myc-DDK-tagged)-Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $614.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POMGNT1
Synonyms gnT-I.2; GNTI.2; GnT I.2; LGMD2O; LGMDR15; MEB; MGAT1.2; RP76
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200176 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACTGGAAGCCCAGCCCCCTCATCAAGCCCTTTGGGGCTCGGAAGAAGCGGAGCTGGTACCTTA
CCTGGAAGTATAAACTGACAAACCAGCGGGCCCTGCGGAGATTCTGTCAGACAGGGGCCGTGCTTTTCCT
GCTGGTGACTGTCATTGTCAATATCAAGTTGATCCTGGACACTCGGCGAGCCATCAGTGAAGCCAATGAA
GACCCAGAGCCAGAGCAAGACTATGATGAGGCCCTAGGCCGCCTGGAGCCCCCACGGCGCAGAGGCAGTG
GTCCCCGGCGGGTCCTGGACGTAGAGGTGTATTCAAGTCGCAGCAAAGTATATGTGGCAGTGGATGGCAC
CACGGTGCTGGAGGATGAGGCCCGGGAGCAGGGCCGGGGCATCCATGTCATTGTCCTCAACCAGGCCACG
GGCCACGTGATGGCAAAACGTGTGTTTGACACGTACTCACCTCATGAGGATGAGGCCATGGTGCTATTCC
TCAACATGGTAGCGCCCGGCCGAGTGCTCATCTGCACTGTCAAGGATGAGGGCTCCTTCCACCTCAAGGA
CACAGCCAAGGCTCTGCTGAGGAGCCTGGGCAGCCAGGCTGGCCCTGCCCTGGGCTGGAGGGACACATGG
GCCTTCGTGGGACGAAAAGGAGGTCCTGTCTTCGGGGAGAAACATTCTAAATCACCTGCCCTCTCTTCCT
GGGGGGACCCAGTCCTGCTGAAGACAGATGTGCCATTGAGCTCAGCAGTAGAGGCAGAGTGCCACTGGGC
AGACACAGAGCTGAACCGTCGCCGCCGGCGCTTCTGCAGCAAAGTTGAGGGCTATGGAAGTGTATGCAGC
TGCAAGGACCCCACACCCATCGAGTTCAGCCCTGACCCACTCCCAGACAACAAGGTCCTCAATGTGCCTG
TGGCTGTCATTGCAGGGAACCGACCCAATTACCTGTACAGGATGCTGCGCTCTCTGCTTTCAGCCCAGGG
GGTGTCTCCTCAGATGATAACAGTTTTCATTGACGGCTACTATGAGGAACCCATGGATGTGGTGGCACTG
TTTGGTCTGAGGGGCATCCAGCATACTCCCATCAGCATCAAGAATGCCCGCGTGTCTCAGCACTACAAGG
CCAGCCTCACTGCCACTTTCAACCTGTTTCCGGAGGCCAAGTTTGCTGTGGTTCTGGAAGAGGACCTGGA
CATTGCTGTGGATTTTTTCAGTTTCCTGAGCCAATCCATCCACCTACTGGAGGAGGATGACAGCCTGTAC
TGCATCTCTGCCTGGAATGACCAGGGGTATGAACACACGGCTGAGGACCCAGCACTACTGTACCGTGTGG
AGACCATGCCTGGGCTGGGCTGGGTGCTCAGGAGGTCCTTGTACAAGGAGGAGCTTGAGCCCAAGTGGCC
TACACCGGAAAAGCTCTGGGATTGGGACATGTGGATGCGGATGCCTGAACAACGCCGGGGCCGAGAGTGC
ATCATCCCTGACGTTTCCCGATCCTACCACTTTGGCATCGTCGGCCTCAACATGAATGGCTACTTTCACG
AGGCCTACTTCAAGAAGCACAAGTTCAACACGGTTCCAGGTGTCCAGCTCAGGAATGTGGACAGTCTGAA
GAAAGAAGCTTATGAAGTGGAAGTTCACAGGCTGCTCAGTGAGGCTGAGGTTCTGGACCACAGCAAGAAC
CCTTGTGAAGACTCTTTCCTGCCAGACACAGAGGGCCACACCTACGTGGCCTTTATTCGAATGGAGAAAG
ATGATGACTTCACCACCTGGACCCAGCTTGCCAAGTGCCTCCATATCTGGGACCTGGATGTGCGTGGCAA
CCATCGGGGCCTGTGGAGATTGTTTCGGAAGAAGAACCACTTCCTGGTGGTGGGGGTCCCGGCTTCCCCC
TACTCAGTGAAGAAGCCACCCTCAGTCACCCCAATTTTCCTGGAGCCACCCCCAAAGGAGGAGGGAGCCC
CAGGAGCCCCAGAACAGACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200176 protein sequence
Red=Cloning site Green=Tags(s)

MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAVLFLLVTVIVNIKLILDTRRAISEANE
DPEPEQDYDEALGRLEPPRRRGSGPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQAT
GHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTW
AFVGRKGGPVFGEKHSKSPALSSWGDPVLLKTDVPLSSAVEAECHWADTELNRRRRRFCSKVEGYGSVCS
CKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVAL
FGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLY
CISAWNDQGYEHTAEDPALLYRVETMPGLGWVLRRSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGREC
IIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEVHRLLSEAEVLDHSKN
PCEDSFLPDTEGHTYVAFIRMEKDDDFTTWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVVGVPASP
YSVKKPPSVTPIFLEPPPKEEGAPGAPEQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017739
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017739.4
RefSeq Size 2753 bp
RefSeq ORF 1983 bp
Locus ID 55624
UniProt ID Q8WZA1
Cytogenetics 1p34.1
Domains GNT-I
Protein Pathways O-Mannosyl glycan biosynthesis
MW 75.2 kDa
Summary This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha linked terminal mannose. Mutations in this gene may be associated with muscle-eye-brain disease and several congenital muscular dystrophies. Alternatively spliced transcript variants that encode different protein isoforms have been described. provided by RefSeq, Feb 2014
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Documents
Resources
"NM_017739" in other vectors (4)
SKU Description Size Price
RC200176L3 Lenti ORF clone of Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1, Myc-DDK-tagged 10 ug
$914.00
RC200176L4 Lenti ORF clone of Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1, mGFP tagged 10 ug
$914.00
RG200176 POMGNT1 (tGFP-tagged) - Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1 10 ug
$489.00 $814.00
SC319371 POMGNT1 (untagged)-Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1 10 ug
$615.00

Plasmid Map for RC200176

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200176
Circular map for RC200176

file-alt Citations

Visualizing intra-Golgi localization and transport by side-averaging Golgi ministacks
Tie, Mahajan, Lu
J Cell Biol (2022) 221 (6) DOI: 10.1083/jcb.202109114
Cited by 43
AI Summary: OriGene products B4GALT7-Myc (#RC200258), B3GALT6-Myc (#MR204731), POMGNT1-Myc (#RC200176), SLC35C1-Myc (#RC200101), and SMS1-Myc (#MR206639) were used as tagged Golgi protein constructs to visualize and analyze the cisternal organization and intra-Golgi transport in this study.
Snippet: … B4GALT7-Myc (#RC200258), B3GALT6-Myc (#MR204731), POMGNT1-Myc (#RC200176), SLC35C1-Myc (#RC200101), and SMS1-Myc (#MR206639) were purchased from Origene. …B4GALT7-Myc (#RC200258), B3GALT6-Myc (#MR204731), POMGNT1-Myc (#RC200176), SLC35C1-Myc (#RC200101), and SMS1-Myc (#MR206639) were purchased from Origene. …
The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack
Tie, Ludwig, Sandin et al
Elife (2018) 7 DOI: 10.7554/eLife.41301
AI Summary: In this study, OriGene products SLC35C1-Myc (Cat. No.: RC200101), β3GalT6-Myc (Cat. No.: MR204731), and β4GalT7-Myc (Cat. No.: RC200258) were used as recombinant DNA reagents to label and study specific Golgi components, enabling the authors to investigate the spatial organization of processing and transport functions within the Golgi complex.
Snippet: le 3 Recombinant DNA reagent β4GalT3-Myc this paper See Supplementary file 3 Recombinant DNA reagent GalT-mCherry PMID: 26764092 Recombinant DNA reagent SLC35C1-Myc OriGene Technologies Inc. … No.: RC200101 Recombinant DNA reagent β3GalT6-Myc OriGene Technologies Inc. … No.: MR204731 Recombinant DNA reagent β4GalT7-Myc OriGene Te;Recombinant DNA reagent SLC35C1-Myc OriGene Technologies Inc. … No.: RC200101 Recombinant DNA reagent β3GalT6-Myc OriGene Technologies Inc. … No.: MR204731 Recombinant DNA reagent β4GalT7-Myc OriGene Technologies Inc. … No.: RC200258
FigureFigureFigure
Showing 1-2 of 2 papers.
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