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POMGNT1 (NM_017739) Human Untagged Clone

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SKU
SC319371
POMGNT1 (untagged)-Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1
  $615.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol POMGNT1
Synonyms gnT-I.2; GNTI.2; GnT I.2; LGMD2O; LGMDR15; MEB; MGAT1.2; RP76
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_017739.2 GGCCGGGGCGGGGCCGCAAGCGGCATGGAGGAGGCGGAGGCCGCGGCGAGCCGGGCCGAG
CAGTGAGGGCCCTAGCGGGGCCCGAGCGGGGCCCGGGGCCCCTAAGCCATTCCTGAAGTC
ATGGGCTGGCCAGGACATTGGTGACCCGCCAATCCGGTATGGACGACTGGAAGCCCAGCC
CCCTCATCAAGCCCTTTGGGGCTCGGAAGAAGCGGAGCTGGTACCTTACCTGGAAGTATA
AACTGACAAACCAGCGGGCCCTGCGGAGATTCTGTCAGACAGGGGCCGTGCTTTTCCTGC
TGGTGACTGTCATTGTCAATATCAAGTTGATCCTGGACACTCGGCGAGCCATCAGTGAAG
CCAATGAAGACCCAGAGCCAGAGCAAGACTATGATGAGGCCCTAGGCCGCCTGGAGCCCC
CACGGCGCAGAGGCAGTGGTCCCCGGCGGGTCCTGGACGTAGAGGTGTATTCAAGTCGCA
GCAAAGTATATGTGGCAGTGGATGGCACCACGGTGCTGGAGGATGAGGCCCGGGAGCAGG
GCCGGGGCATCCATGTCATTGTCCTCAACCAGGCCACGGGCCACGTGATGGCAAAACGTG
TGTTTGACACGTACTCACCTCATGAGGATGAGGCCATGGTGCTATTCCTCAACATGGTAG
CGCCCGGCCGAGTGCTCATCTGCACTGTCAAGGATGAGGGCTCCTTCCACCTCAAGGACA
CAGCCAAGGCTCTGCTGAGGAGCCTGGGCAGCCAGGCTGGCCCTGCCCTGGGCTGGAGGG
ACACATGGGCCTTCGTGGGACGAAAAGGAGGTCCTGTCTTCGGGGAGAAACATTCTAAAT
CACCTGCCCTCTCTTCCTGGGGGGACCCAGTCCTGCTGAAGACAGATGTGCCATTGAGCT
CAGCAGTAGAGGCAGAGTGCCACTGGGCAGACACAGAGCTGAACCGTCGCCGCCGGCGCT
TCTGCAGCAAAGTTGAGGGCTATGGAAGTGTATGCAGCTGCAAGGACCCCACACCCATCG
AGTTCAGCCCTGACCCACTCCCAGACAACAAGGTCCTCAATGTGCCTGTGGCTGTCATTG
CAGGGAACCGACCCAATTACCTGTACAGGATGCTGCGCTCTCTGCTTTCAGCCCAGGGGG
TGTCTCCTCAGATGATAACAGTTTTCATTGACGGCTACTATGAGGAACCCATGGATGTGG
TGGCACTGTTTGGTCTGAGGGGCATCCAGCATACTCCCATCAGCATCAAGAATGCCCGCG
TGTCTCAGCACTACAAGGCCAGCCTCACTGCCACTTTCAACCTGTTTCCGGAGGCCAAGT
TTGCTGTGGTTCTGGAAGAGGACCTGGACATTGCTGTGGATTTTTTCAGTTTCCTGAGCC
AATCCATCCACCTACTGGAGGAGGATGACAGCCTGTACTGCATCTCTGCCTGGAATGACC
AGGGGTATGAACACACGGCTGAGGACCCAGCACTACTGTACCGTGTGGAGACCATGCCTG
GGCTGGGCTGGGTGCTCAGGAGGTCCTTGTACAAGGAGGAGCTTGAGCCCAAGTGGCCTA
CACCGGAAAAGCTCTGGGATTGGGACATGTGGATGCGGATGCCTGAACAACGCCGGGGCC
GAGAGTGCATCATCCCTGACGTTTCCCGATCCTACCACTTTGGCATCGTCGGCCTCAACA
TGAATGGCTACTTTCACGAGGCCTACTTCAAGAAGCACAAGTTCAACACGGTTCCAGGTG
TCCAGCTCAGGAATGTGGACAGTCTGAAGAAAGAAGCTTATGAAGTGGAAGTTCACAGGC
TGCTCAGTGAGGCTGAGGTTCTGGACCACAGCAAGAACCCTTGTGAAGACTCTTTCCTGC
CAGACACAGAGGGCCACACCTACGTGGCCTTTATTCGAATGGAGAAAGATGATGACTTCA
CCACCTGGACCCAGCTTGCCAAGTGCCTCCATATCTGGGACCTGGATGTGCGTGGCAACC
ATCGGGGCCTGTGGAGATTGTTTCGGAAGAAGAACCACTTCCTGGTGGTGGGGGTCCCGG
CTTCCCCCTACTCAGTGAAGAAGCCACCCTCAGTCACCCCAATTTTCCTGGAGCCACCCC
CAAAGGAGGAGGGAGCCCCAGGAGCCCCAGAACAGACATGAGACCTCCTCCAGGACCCTG
CGGGGCTGGGTACTGTGTACCCCCAGGCTGGCTAGCCCTTCCCTCCATCCTGTAGGATTT
TGTAGATGCTGGTAGGGGCTGGGGCTACCTTGTTTTTAACATGAGACTTAATTACTAACT
CCAAGGGGAGGGTTCCCCTGCTCCAACACCCCGTTCCTGAGTTAAAAGTCTATTTATTTA
CTTCCTTGTTGGAGAAGGGCAGGAGAGTACCTGGGAATCATTACGATCCCTAGCAGCTCA
TCCTGCCCTTTGAATACCCTCACTTTCCAGGCCTGGCTCAGAATCTAACCTATTTATTGA
CTGTCCTGAGGGCCTTGAAAACAGGCCGAACCTGGAGGGCCTGGATTTCTTTTTGGGCTG
GAATGCTGCCCTGAGGGTGGGGCTGGCTCTTACTCAGGAAACTGCTGTGCCCAACCCATG
GACAGGCCCAGCTGGGGCCCACATGCTGACACAGACTCACTCAGAGACCCTTAGACACTG
GACCAGGCCTCCTCTCAGCCTTCTCTTTGTCCAGATTTCCAAAGCTGGATAAGTTGGTCA
TTGATTAAAAAAGGAGAAGCCCTCTGAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_017739
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017739.2, NP_060209.2
RefSeq Size 2753 bp
RefSeq ORF 1983 bp
Locus ID 55624
UniProt ID Q8WZA1
Cytogenetics 1p34.1
Domains GNT-I
Protein Pathways O-Mannosyl glycan biosynthesis
Summary This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha linked terminal mannose. Mutations in this gene may be associated with muscle-eye-brain disease and several congenital muscular dystrophies. Alternatively spliced transcript variants that encode different protein isoforms have been described. provided by RefSeq, Feb 2014
Transcript Variant: This variant (1) represents the predominant transcript and encodes the shorter isoform (1).
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Documents
Resources
"NM_017739" in other vectors (4)
SKU Description Size Price
RC200176 POMGNT1 (Myc-DDK-tagged)-Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1 10 ug
$614.00
RC200176L3 Lenti ORF clone of Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1, Myc-DDK-tagged 10 ug
$914.00
RC200176L4 Lenti ORF clone of Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1, mGFP tagged 10 ug
$914.00
RG200176 POMGNT1 (tGFP-tagged) - Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1 10 ug
$489.00 $814.00

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