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IMP3 (NM_018285) Human Tagged ORF Clone

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SKU
RC200112
IMP3 (Myc-DDK-tagged)-Human IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IMP3
Synonyms BRMS2; C15orf12; MRPS4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200112 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCGGAAGCTTAAGTTCCACGAGCAGAAGCTGCTGAAGCAGGTGGACTTCCTGAACTGGGAGGTCA
CCGACCACAACCTGCACGAGCTGCGCGTGCTGCGGCGTTACCGGCTGCAGCGGCGGGAGGACTACACGCG
CTACAACCAGCTGAGCCGTGCCGTGCGTGAGCTGGCGCGGCGCCTGCGCGACCTGCCCGAACGCGACCAG
TTCCGCGTGCGCGCTTCGGCCGCGCTGCTGGACAAGCTGTATGCTCTCGGCTTGGTGCCCACGCGCGGTT
CGCTGGAGCTCTGCGACTTCGTCACGGCCTCGTCCTTCTGCCGCCGCCGCCTCCCCACCGTGCTCCTCAA
GCTGCGCATGGCGCAGCACCTTCAGGCTGCAGTGGCCTTTGTGGAGCAAGGGCACGTACGCGTGGGCCCT
GACGTGGTTACCGACCCCGCCTTCCTTGTCACGCGCAGCATGGAGGACTTTGTCACTTGGGTGGACTCGT
CCAAGATCAAGCGGCACGTGCTAGAGTACAATGAGGAGCGCGATGACTTCGATCTGGAAGCC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200112 protein sequence
Red=Cloning site Green=Tags(s)

MVRKLKFHEQKLLKQVDFLNWEVTDHNLHELRVLRRYRLQRREDYTRYNQLSRAVRELARRLRDLPERDQ
FRVRASAALLDKLYALGLVPTRGSLELCDFVTASSFCRRRLPTVLLKLRMAQHLQAAVAFVEQGHVRVGP
DVVTDPAFLVTRSMEDFVTWVDSSKIKRHVLEYNEERDDFDLEA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_018285
ORF Size 552 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018285.1
RefSeq Size 1255 bp
RefSeq ORF 555 bp
Locus ID 55272
UniProt ID Q9NV31
Cytogenetics 15q24.2
Domains S4
MW 21.9 kDa
Summary This gene encodes the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the U3 snoRNP complex. The protein contains an S4 domain. provided by RefSeq, Jul 2008
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"NM_018285" in other vectors (4)
SKU Description Size Price
RC200112L3 Lenti ORF clone of Human IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP3), Myc-DDK-tagged 10 ug
$600.00
RC200112L4 Lenti ORF clone of Human IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP3), mGFP tagged 10 ug
$600.00
RG200112 IMP3 (tGFP-tagged) - Human IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP3) 10 ug
$489.00 $500.00
SC113595 IMP3 (untagged)-Human IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP3) 10 ug
$300.00

Plasmid Map for RC200112

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200112
Circular map for RC200112

file-alt Citations

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