BFAR (NM_016561) Human Tagged ORF Clone

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SKU
RC200078
BFAR (Myc-DDK-tagged)-Human bifunctional apoptosis regulator (BFAR)
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BFAR
Synonyms BAR; RNF47
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200078 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAACCTCAGAAAAGCTATGTGAACACAATGGACCTTGAGAGAGATGAACCTCTCAAAAGCACCG
GCCCTCAGATTTCTGTTAGTGAATTTTCTTGCCACTGCTGCTACGACATCCTGGTTAACCCCACCACCTT
GAACTGTGGGCACAGCTTCTGCCGTCACTGCCTTGCTTTATGGTGGGCATCTTCAAAGAAAACAGAATGT
CCAGAATGCAGAGAAAAATGGGAAGGTTTCCCCAAAGTCAGTATTCTCCTCAGGGATGCCATTGAAAAGT
TATTTCCTGATGCCATTAGACTGAGATTTGAAGACATTCAGCAGAATAATGACATAGTCCAAAGTCTTGC
AGCCTTTCAGAAATATGGGAATGATCAGATTCCTTTAGCTCCTAACACAGGCCGAGCGAATCAGCAGATG
GGAGGGGGATTCTTTTCCGGTGTGCTCACAGCTTTAACTGGAGTGGCAGTGGTCCTGCTCGTCTATCACT
GGAGCAGCAGGGAATCTGAACACGACCTCCTGGTCCACAAGGCTGTGGCCAAATGGACGGCGGAAGAAGT
TGTCCTCTGGCTGGAGCAGCTGGGCCCTTGGGCATCTCTTTACAGGGAAAGGTTTTTATCTGAACGAGTA
AATGGAAGGTTGCTTTTAACTTTGACAGAGGAAGAATTTTCCAAGACGCCCTATACCATAGAAAACAGCA
GCCACAGGAGAGCCATCCTCATGGAGCTAGAACGTGTCAAAGCATTAGGCGTGAAGCCCCCCCAGAATCT
CTGGGAATATAAGGCTGTGAACCCAGGCAGGTCCCTGTTCCTGCTATACGCCCTCAAGAGCTCCCCCAGG
CTGAGTCTGCTCTACCTGTACCTGTTTGACTACACCGACACCTTCCTACCTTTCATCCACACCATCTGCC
CTCTGCAAGAAGACAGCTCTGGGGAGGACATCGTCACCAAGCTTCTGGATCTTAAGGAGCCTACGTGGAA
GCAGTGGAGAGAGTTCCTGGTCAAATACTCCTTCCTTCCATACCAGCTGATTGCTGAGTTTGCTTGGGAC
TGGTTGGAGGTCCATTACTGGACATCACGGTTTCTCATCATCAATGCTATGTTACTCTCAGTTCTGGAAT
TATTCTCCTTTTGGAGAATCTGGTCGAGAAGTGAACTGAAGACCGTGCCTCAGAGGATGTGGAGCCATTT
CTGGAAAGTATCAACGCAGGGGCTTTTTGTGGCCATGTTCTGGCCCCTCATCCCTCAGTTTGTTTGCAAC
TGTTTGTTTTACTGGGCCCTGTACTTTAACCCAATTATTAACATTGATCTTGTGGTCAAGGAACTCCGGC
GGCTGGAAACCCAGGTGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200078 protein sequence
Red=Cloning site Green=Tags(s)

MEEPQKSYVNTMDLERDEPLKSTGPQISVSEFSCHCCYDILVNPTTLNCGHSFCRHCLALWWASSKKTEC
PECREKWEGFPKVSILLRDAIEKLFPDAIRLRFEDIQQNNDIVQSLAAFQKYGNDQIPLAPNTGRANQQM
GGGFFSGVLTALTGVAVVLLVYHWSSRESEHDLLVHKAVAKWTAEEVVLWLEQLGPWASLYRERFLSERV
NGRLLLTLTEEEFSKTPYTIENSSHRRAILMELERVKALGVKPPQNLWEYKAVNPGRSLFLLYALKSSPR
LSLLYLYLFDYTDTFLPFIHTICPLQEDSSGEDIVTKLLDLKEPTWKQWREFLVKYSFLPYQLIAEFAWD
WLEVHYWTSRFLIINAMLLSVLELFSFWRIWSRSELKTVPQRMWSHFWKVSTQGLFVAMFWPLIPQFVCN
CLFYWALYFNPIINIDLVVKELRRLETQVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016561
ORF Size 1350 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016561.3
RefSeq Size 3060 bp
RefSeq ORF 1353 bp
Locus ID 51283
UniProt ID Q9NZS9
Cytogenetics 16p13.12
Domains RING, SAM
Protein Families Druggable Genome, Transmembrane
MW 52.7 kDa
Summary Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016561" in other vectors (6)
SKU Description Size Price
RC200078L1 Lenti ORF clone of Human bifunctional apoptosis regulator (BFAR), Myc-DDK-tagged 10 ug
$757.00
RC200078L2 Lenti ORF clone of Human bifunctional apoptosis regulator (BFAR), mGFP tagged 10 ug
$757.00
RC200078L3 Lenti ORF clone of Human bifunctional apoptosis regulator (BFAR), Myc-DDK-tagged 10 ug
$757.00
RC200078L4 Lenti ORF clone of Human bifunctional apoptosis regulator (BFAR), mGFP tagged 10 ug
$757.00
RG200078 BFAR (tGFP-tagged) - Human bifunctional apoptosis regulator (BFAR) 10 ug
$489.00 $657.00
SC319484 BFAR (untagged)-Human bifunctional apoptosis regulator (BFAR) 10 ug
$457.00

Plasmid Map for RC200078

Download sequence for

Cloning scheme for RC200078

Circular map for RC200078

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