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SND1 (NM_014390) Human Tagged ORF Clone

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SKU
RC200059
SND1 (Myc-DDK-tagged)-Human staphylococcal nuclease and tudor domain containing 1 (SND1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $833.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SND1
Synonyms p100; TDRD11; Tudor-SN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200059 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCTCCGCGCAGAGCGGCGGCTCCTCCGGGGGACCCGCGGTCCCCACCGTGCAGCGGGGCATCA
TCAAGATGGTCCTCTCAGGGTGCGCCATCATTGTCCGAGGTCAGCCTCGTGGTGGGCCTCCTCCTGAGCG
GCAGATCAACCTCAGCAACATTCGTGCTGGAAATCTTGCTCGCCGGGCAGCCGCCACACAACCTGATGCA
AAGGATACCCCTGATGAGCCCTGGGCATTTCCAGCTCGAGAGTTCCTTCGAAAGAAGCTGATTGGGAAGG
AAGTCTGTTTCACGATAGAAAACAAGACTCCCCAGGGGCGAGAGTATGGCATGATCTACCTTGGAAAAGA
TACCAATGGGGAAAACATTGCAGAATCACTGGTTGCAGAGGGCTTAGCCACCCGGAGAGAAGGCATGAGA
GCTAATAATCCTGAGCAGAACCGGCTTTCAGAATGTGAAGAACAAGCAAAGGCAGCCAAGAAAGGGATGT
GGAGTGAGGGGAACGGTTCACATACTATCCGGGATCTCAAGTATACCATTGAAAACCCAAGGCACTTTGT
GGACTCACACCACCAGAAGCCTGTTAATGCTATCATCGAGCATGTGCGGGACGGCAGTGTGGTCAGGGCC
CTGCTCCTCCCAGATTACTACCTGGTTACAGTCATGCTGTCAGGCATCAAGTGCCCAACTTTTCGACGGG
AAGCAGATGGCAGTGAAACTCCAGAGCCTTTTGCTGCAGAAGCCAAATTTTTCACTGAGTCGCGACTGCT
TCAGAGAGATGTTCAGATCATTCTGGAGAGCTGCCACAACCAGAACATTCTGGGTACCATCCTTCATCCA
AATGGCAACATCACAGAGCTCCTCCTGAAGGAAGGTTTCGCACGCTGTGTGGACTGGTCGATTGCAGTTT
ACACCCGGGGCGCAGAAAAGCTGAGGGCGGCAGAGAGGTTTGCCAAAGAGCGCAGGCTGAGAATATGGAG
AGACTATGTGGCTCCCACAGCTAATTTGGACCAAAAGGACAAGCAGTTTGTTGCCAAGGTGATGCAGGTT
CTGAATGCTGATGCCATTGTTGTGAAGCTGAACTCAGGCGATTACAAGACGATTCACCTGTCCAGCATCC
GACCACCGAGGCTGGAGGGGGAGAACACCCAGGATAAGAACAAGAAACTGCGTCCCCTGTATGACATTCC
TTACATGTTTGAGGCCCGGGAATTTCTTCGAAAAAAGCTTATTGGGAAGAAGGTCAATGTGACGGTGGAC
TACATTAGACCAGCCAGCCCAGCCACAGAGACAGTGCCTGCCTTTTCAGAGCGTACCTGTGCCACTGTCA
CCATTGGAGGAATAAACATTGCTGAGGCTCTTGTCAGCAAAGGTCTAGCCACAGTGATCAGATACCGGCA
GGATGATGACCAGAGATCATCACACTACGATGAACTGCTTGCTGCAGAGGCCAGAGCTATTAAGAATGGC
AAAGGATTGCATAGCAAGAAGGAAGTGCCTATCCACCGTGTTGCAGATATATCTGGGGATACCCAAAAAG
CAAAGCAGTTCCTGCCTTTTCTTCAGCGGGCAGGTCGTTCTGAAGCTGTGGTGGAATACGTCTTCAGTGG
TTCTCGTCTCAAACTCTATTTGCCAAAGGAAACTTGCCTTATCACCTTCTTGCTTGCAGGCATTGAATGC
CCCAGAGGAGCCCGAAACCTCCCAGGCTTGGTGCAGGAAGGAGAGCCCTTCAGCGAGGAAGCTACACTTT
TCACCAAGGAACTGGTGCTGCAGCGAGAGGTGGAGGTGGAGGTGGAGAGCATGGACAAGGCCGGCAACTT
TATCGGCTGGCTGCACATCGACGGTGCCAACCTGTCCGTCCTGCTGGTGGAGCACGCGCTCTCCAAGGTC
CACTTCACCGCCGAACGCAGCTCCTACTACAAGTCCCTGCTGTCTGCCGAGGAGGCCGCAAAGCAGAAGA
AAGAGAAGGTCTGGGCCCACTATGAGGAGCAGCCCGTGGAGGAGGTGATGCCAGTGCTGGAGGAGAAGGA
GCGATCTGCTAGCTACAAGCCCGTGTTTGTGACCGAGATCACTGATGACCTGCACTTCTACGTGCAGGAT
GTGGAGACCGGCACCCAGTTGGAGAAGCTGATGGAGAACATGCGCAATGACATTGCCAGTCACCCCCCTG
TAGAGGGCTCCTATGCCCCCCGCAGGGGAGAGTTCTGCATTGCCAAATTTGTAGATGGAGAATGGTACCG
TGCCCGAGTAGAGAAAGTCGAGTCTCCTGCCAAAATACATGTCTTCTACATTGACTACGGCAACAGAGAG
GTCCTGCCATCCACCCGCCTGGGTACCCTATCACCTGCCTTCAGCACTCGGGTGCTGCCAGCTCAAGCCA
CGGAGTATGCCTTCGCCTTCATCCAGGTGCCCCAAGATGATGATGCCCGCACGGACGCCGTGGACAGCGT
AGTTCGGGATATCCAGAACACTCAGTGCCTGCTCAACGTGGAACACCTGAGTGCCGGCTGCCCCCATGTC
ACCCTGCAGTTTGCAGATTCCAAGGGCGATGTGGGGCTGGGCTTGGTGAAGGAAGGGCTGGTCATGGTGG
AGGTGCGCAAGGAGAAACAGTTCCAGAAAGTGATCACAGAATACCTGAATGCCCAAGAGTCAGCCAAGAG
CGCCAGGCTGAACCTGTGGCGCTATGGAGACTTTCGAGCTGATGATGCAGACGAATTTGGCTACAGCCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200059 protein sequence
Red=Cloning site Green=Tags(s)

MASSAQSGGSSGGPAVPTVQRGIIKMVLSGCAIIVRGQPRGGPPPERQINLSNIRAGNLARRAAATQPDA
KDTPDEPWAFPAREFLRKKLIGKEVCFTIENKTPQGREYGMIYLGKDTNGENIAESLVAEGLATRREGMR
ANNPEQNRLSECEEQAKAAKKGMWSEGNGSHTIRDLKYTIENPRHFVDSHHQKPVNAIIEHVRDGSVVRA
LLLPDYYLVTVMLSGIKCPTFRREADGSETPEPFAAEAKFFTESRLLQRDVQIILESCHNQNILGTILHP
NGNITELLLKEGFARCVDWSIAVYTRGAEKLRAAERFAKERRLRIWRDYVAPTANLDQKDKQFVAKVMQV
LNADAIVVKLNSGDYKTIHLSSIRPPRLEGENTQDKNKKLRPLYDIPYMFEAREFLRKKLIGKKVNVTVD
YIRPASPATETVPAFSERTCATVTIGGINIAEALVSKGLATVIRYRQDDDQRSSHYDELLAAEARAIKNG
KGLHSKKEVPIHRVADISGDTQKAKQFLPFLQRAGRSEAVVEYVFSGSRLKLYLPKETCLITFLLAGIEC
PRGARNLPGLVQEGEPFSEEATLFTKELVLQREVEVEVESMDKAGNFIGWLHIDGANLSVLLVEHALSKV
HFTAERSSYYKSLLSAEEAAKQKKEKVWAHYEEQPVEEVMPVLEEKERSASYKPVFVTEITDDLHFYVQD
VETGTQLEKLMENMRNDIASHPPVEGSYAPRRGEFCIAKFVDGEWYRARVEKVESPAKIHVFYIDYGNRE
VLPSTRLGTLSPAFSTRVLPAQATEYAFAFIQVPQDDDARTDAVDSVVRDIQNTQCLLNVEHLSAGCPHV
TLQFADSKGDVGLGLVKEGLVMVEVRKEKQFQKVITEYLNAQESAKSARLNLWRYGDFRADDADEFGYSR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014390
ORF Size 2730 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014390.4
RefSeq Size 3522 bp
RefSeq ORF 2733 bp
Locus ID 27044
UniProt ID Q7KZF4
Cytogenetics 7q32.1
Domains SNase, TUDOR
Protein Families Druggable Genome, Transcription Factors
MW 102 kDa
Summary This gene encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC). provided by RefSeq, Jul 2016
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Documents
Resources
"NM_014390" in other vectors (8)
SKU Description Size Price
RC200059L1 Lenti ORF clone of Human staphylococcal nuclease and tudor domain containing 1 (SND1), Myc-DDK-tagged 10 ug
$1,133.00
RC200059L2 Lenti ORF clone of Human staphylococcal nuclease and tudor domain containing 1 (SND1), mGFP tagged 10 ug
$1,133.00
RC200059L3 Lenti ORF clone of Human staphylococcal nuclease and tudor domain containing 1 (SND1), Myc-DDK-tagged 10 ug
$1,133.00
RC200059L4 Lenti ORF clone of Human staphylococcal nuclease and tudor domain containing 1 (SND1), mGFP tagged 10 ug
$1,133.00
RG200059 SND1 (tGFP-tagged) - Human staphylococcal nuclease and tudor domain containing 1 (SND1) 10 ug
$1,033.00
SC111238 SND1 (untagged)-Human staphylococcal nuclease and tudor domain containing 1 (SND1) 10 ug
$811.00
SC318380 SND1 (untagged)-Human staphylococcal nuclease and tudor domain containing 1 (SND1) 10 ug
$917.00
SC320306 SND1 (untagged)-Human staphylococcal nuclease and tudor domain containing 1 (SND1) 10 ug
$834.00

Plasmid Map for RC200059

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200059
Circular map for RC200059

file-alt Citations

Disruption of SND1-MTDH Interaction by a High Affinity Peptide Results in SND1 Degradation and Cytotoxicity to Breast Cancer Cells In Vitro and In Vivo
Li, He, Chen et al
Mol Cancer Ther (2020)
AI Summary: OriGene’s pCMV6-Entry-SND1-cMyc-DDK plasmid (#RC200059) was used to stably transfect breast cancer cells, enabling the study of SND1 function and its disruption by therapeutic peptides in this research.
Snippet: Transfection 166 Stable transfection of MDA-MB-231-GFP-Red-FLuc cell line with 167 pCMV6-Entry-SND1-cMyc-DDK plasmid (#RC200059, Origene) was 168 conducted using lipofectamine 3000 reagent (Thermo Fisher) according to the 169 instructions of the manufacturers
Staphylococcal nuclease and tudor domain containing 1 (SND1) promotes hepatocarcinogenesis by inhibiting monoglyceride lipase (MGLL)
Rajasekaran, Jariwala, Mendoza et al
J. Biol. Chem. (2016)
AI Summary: In this study, the OriGene FLAG-Myc-tagged SND1 expression construct was used to investigate the role of SND1 in hepatocarcinogenesis by enabling functional and interaction analyses of SND1 in hepatocellular carcinoma cells.
Snippet: Plasmids: FLAG-tagged expression constructs for full-length SND1, and SN and tudor domains of SND1 were kindly provided by Dr. Kirsi Paukku in University of Helsinki. FLAG-Myc- tagged SND1 expression construct was obtained from Origene. Human MGLL cDNA ... Cite SaveSaving
Suppression of miR-184 in malignant gliomas upregulates SND1 and promotes tumor aggressiveness
Emdad, Janjic, Alzubi et al
Neuro-oncology (2014)
Cited by 82
AI Summary: OriGene’s pCMV6-ENTRY SND1 expression vector with C-terminal Myc and FLAG tags was used in this study to investigate the functional role of SND1 in glioma cells.
Snippet: Plasmid Constructions, Transfections, and Stable Clone Generation. SND1 expression vector in pCMV6-ENTRY with C-terminal Myc and FLAG tags was obtained from Origene Technologies. The shRNAs, designed to knock down SND1, were from Santa Cruz ... Cite SaveSaving
Showing 1-3 of 3 papers.
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