YTHDF2 (NM_016258) Human Tagged ORF Clone

SKU
RC200038
YTHDF2 (Myc-DDK-tagged)-Human YTH domain family, member 2 (YTHDF2), transcript variant 1
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Specifications
Product Data
Target Symbol YTHDF2
Synonyms CAHL; DF2; HGRG8; NY-REN-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200038 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCCAGCAGCCTCTTGGAGCAGAGACCAAAAGGTCAAGGAAACAAAGTACAAAATGGATCTGTAC
ATCAAAAGGATGGATTAAACGATGATGATTTTGAACCTTACTTGAGTCCACAGGCAAGGCCCAATAATGC
ATATACTGCCATGTCAGATTCCTACTTACCCAGTTACTACAGTCCCTCCATTGGCTTCTCCTATTCTTTG
GGTGAAGCTGCTTGGTCTACGGGGGGTGACACAGCCATGCCCTACTTAACTTCTTATGGACAGCTGAGCA
ACGGAGAGCCCCACTTCCTACCAGATGCAATGTTTGGGCAACCAGGAGCCCTAGGTAGCACTCCATTTCT
TGGTCAGCATGGTTTTAATTTCTTTCCCAGTGGGATTGACTTCTCAGCATGGGGAAATAACAGTTCTCAG
GGACAGTCTACTCAGAGCTCTGGATATAGTAGCAATTATGCTTATGCACCTAGCTCCTTAGGTGGAGCCA
TGATTGATGGACAGTCAGCTTTTGCCAATGAGACCCTCAATAAGGCTCCTGGCATGAATACTATAGACCA
AGGGATGGCAGCACTGAAGTTGGGTAGCACAGAAGTTGCAAGCAATGTTCCAAAAGTTGTAGGTTCTGCT
GTTGGTAGCGGGTCCATTACTAGTAACATCGTGGCTTCCAATAGTTTGCCTCCAGCCACCATTGCTCCTC
CAAAACCAGCATCTTGGGCTGATATTGCTAGCAAGCCTGCAAAACAGCAACCTAAACTGAAGACCAAGAA
TGGCATTGCAGGGTCAAGTCTTCCGCCACCCCCGATAAAGCATAACATGGATATTGGAACTTGGGATAAC
AAGGGTCCCGTTGCAAAAGCCCCCTCACAGGCTTTGGTTCAGAATATAGGTCAGCCAACCCAGGGGTCTC
CTCAGCCTGTAGGTCAGCAGGCTAACAATAGCCCACCAGTGGCTCAGGCATCAGTAGGGCAACAGACACA
GCCATTGCCTCCACCTCCACCACAGCCTGCCCAGCTTTCAGTCCAGCAACAGGCAGCTCAGCCAACCCGC
TGGGTAGCACCTCGGAACCGTGGCAGTGGGTTCGGTCATAATGGGGTGGATGGTAATGGAGTAGGACAGT
CTCAGGCTGGTTCTGGATCTACTCCTTCAGAACCCCACCCAGTGTTGGAGAAGCTTCGGTCCATTAATAA
CTATAACCCCAAAGATTTTGACTGGAATCTGAAACATGGCCGGGTTTTCATCATTAAGAGCTACTCTGAG
GACGATATTCACCGTTCCATTAAGTATAATATTTGGTGCAGCACAGAGCATGGTAACAAGAGACTGGATG
CTGCTTATCGTTCCATGAACGGGAAAGGCCCCGTTTACTTACTTTTCAGTGTCAACGGCAGTGGACACTT
CTGTGGCGTGGCAGAAATGAAATCTGCTGTGGACTACAACACATGTGCAGGTGTGTGGTCCCAGGACAAA
TGGAAGGGTCGTTTTGATGTCAGGTGGATTTTTGTGAAGGACGTTCCCAATAGCCAACTGCGACACATTC
GCCTAGAGAACAACGAGAATAAACCAGTGACCAACTCTAGGGACACTCAGGAAGTGCCTCTGGAAAAGGC
TAAGCAGGTGTTGAAAATTATAGCCAGCTACAAGCACACCACTTCCATTTTTGATGACTTCTCACACTAT
GAGAAACGCCAAGAGGAAGAAGAAAGTGTTAAAAAGGAACGTCAAGGTCGTGGGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200038 protein sequence
Red=Cloning site Green=Tags(s)

MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSL
GEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQ
GQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSA
VGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDN
KGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTR
WVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSE
DDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDK
WKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHY
EKRQEEEESVKKERQGRGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_016258
ORF Size 1737 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016258.3
RefSeq Size 3073 bp
RefSeq ORF 1740 bp
Locus ID 51441
UniProt ID Q9Y5A9
Cytogenetics 1p35.3
Domains YTH
MW 62.3 kDa
Summary This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]
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Citations

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