YTHDF2 (NM_016258) Human Tagged ORF Clone
SKU
RC200038
YTHDF2 (Myc-DDK-tagged)-Human YTH domain family, member 2 (YTHDF2), transcript variant 1
$539.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | YTHDF2 |
| Synonyms | CAHL; DF2; HGRG8; NY-REN-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016258 |
| ORF Size | 1737 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016258.3 |
| RefSeq Size | 3073 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 51441 |
| UniProt ID | Q9Y5A9 |
| Cytogenetics | 1p35.3 |
| Domains | YTH |
| MW | 62.3 kDa |
| Summary | This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. provided by RefSeq, Oct 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016258" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200038L3 | Lenti ORF clone of Human YTH domain family, member 2 (YTHDF2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$839.00
|
|
| RC200038L4 | Lenti ORF clone of Human YTH domain family, member 2 (YTHDF2), transcript variant 1, mGFP tagged | 10 ug |
$839.00
|
|
| RG200038 | YTHDF2 (tGFP-tagged) - Human YTH domain family, member 2 (YTHDF2), transcript variant 1 | 10 ug |
$489.00
$739.00
|
|
| SC317991 | YTHDF2 (untagged)-Human YTH domain family, member 2 (YTHDF2), transcript variant 1 | 10 ug |
$594.00
|
|
| SC319366 | YTHDF2 (untagged)-Human YTH domain family, member 2 (YTHDF2), transcript variant 1 | 10 ug |
$540.00
|
|
Citations
YTHDF2 enhances proliferation and metastasis of nasopharyngeal carcinoma by mediating m6A modification in destabilizing FOXO1 mRNA
Yu, Wei, Peng et al
Cancer Biol Ther (2025) 26 (1), 2582349 DOI: 10.1080/15384047.2025.2582349
Yu, Wei, Peng et al
Cancer Biol Ther (2025) 26 (1), 2582349 DOI: 10.1080/15384047.2025.2582349
AI Summary: OriGene products contributed to this study by providing the PCMV6-entry-YTHDF2 plasmid and the empty vector plasmid (PS100001), which were used to manipulate YTHDF2 expression in nasopharyngeal carcinoma cells to investigate its role in cancer progression.
Snippet: ibco (America, C11995500BT), and FBS was acquired from Sigma (Australia, F8318). PCMV6-entry-YTHDF2 plasmid (China, Wuxi, RC200038) and empty vector plasmid (China, Wuxi, PS100001) were obtained from OriGene Technologies. FOXO1 antibody (America, 2880) was procured from CST, and YTHDF2 antibody (China, Wuhan, Polyclonal Antibody for WB, IHC, IP, 24744−1-AP) and GAPDH antibody (China, Wuhan, Mono
Cap-independent mRNA translation is upregulated in long-lived endocrine mutant mice
Ozkurede, Kala, Johnson et al
J. Mol. Endocrinol. (2019) 63 (2), 123-138 DOI: 10.1530/JME-19-0021
Ozkurede, Kala, Johnson et al
J. Mol. Endocrinol. (2019) 63 (2), 123-138 DOI: 10.1530/JME-19-0021
YTH domain family 2 orchestrates epithelial-mesenchymal transition/proliferation dichotomy in pancreatic cancer cells
Chen, Sun, Xu et al
Cell Cycle (2017)
Chen, Sun, Xu et al
Cell Cycle (2017)
Cited by 213
AI Summary: OriGene’s pCMV6-Entry plasmid was used as a vector for cloning in this study, enabling the researchers to manipulate gene expression and investigate the role of YTHDF2 in pancreatic cancer cell proliferation and migration.
Snippet: GTTTTCCCACGACCTTGACGTTCCTT-3 0 , and then inserted into the AsiSI and MluI site of the pCMV6-Entry plas- mid (Origene) and ligated into the vector
Showing 1-3 of 3 papers.
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