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EMC9 (NM_016049) Human Tagged ORF Clone

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SKU
RC200034
EMC9 (Myc-DDK-tagged)-Human family with sequence similarity 158, member A (FAM158A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EMC9
Synonyms C14orf122; CGI-112; FAM158A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200034 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGGTGGAGATCTCGGCCCTGGCCTACGTGAAGATGTGCCTGCATGCTGCCCGGTACCCACACG
CCGCAGTCAACGGGCTGTTTTTGGCGCCAGCGCCGCGGTCTGGAGAATGCCTGTGCCTCACCGACTGTGT
GCCCCTCTTCCACAGCCACCTGGCCCTGTCCGTCATGTTGGAGGTCGCCCTCAACCAGGTGGATGTGTGG
GGAGCACAGGCCGGTCTGGTGGTGGCTGGTTACTACCATGCCAATGCAGCTGTGAACGATCAGAGCCCTG
GGCCCCTGGCCTTGAAAATTGCTGGGCGAATTGCAGAATTCTTCCCTGATGCAGTACTTATTATGTTGGA
TAATCAGAAACTGGTGCCTCAGCCTCGTGTGCCCCCGGTCATCGTCCTGGAGAACCAAGGTCTCCGCTGG
GTCCCTAAGGATAAGAACTTAGTGATGTGGAGGGACTGGGAAGAGTCACGGCAGATGGTGGGAGCTCTAC
TGGAAGATCGGGCCCACCAGCACCTTGTGGACTTTGACTGCCACCTTGATGACATCCGGCAGGACTGGAC
CAACCAGCGGCTCAACACTCAAATCACCCAGTGGGTTGGTCCCACTAATGGAAATGGAAATGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200034 protein sequence
Red=Cloning site Green=Tags(s)

MGEVEISALAYVKMCLHAARYPHAAVNGLFLAPAPRSGECLCLTDCVPLFHSHLALSVMLEVALNQVDVW
GAQAGLVVAGYYHANAAVNDQSPGPLALKIAGRIAEFFPDAVLIMLDNQKLVPQPRVPPVIVLENQGLRW
VPKDKNLVMWRDWEESRQMVGALLEDRAHQHLVDFDCHLDDIRQDWTNQRLNTQITQWVGPTNGNGNA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016049
ORF Size 624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016049.4
RefSeq Size 896 bp
RefSeq ORF 627 bp
Locus ID 51016
UniProt ID Q9Y3B6
Cytogenetics 14q12
Domains UPF0172
MW 23.1 kDa
Summary Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).UniProtKB/Swiss-Prot Function
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"NM_016049" in other vectors (4)
SKU Description Size Price
RC200034L3 Lenti ORF clone of Human family with sequence similarity 158, member A (FAM158A), Myc-DDK-tagged 10 ug
$600.00
RC200034L4 Lenti ORF clone of Human family with sequence similarity 158, member A (FAM158A), mGFP tagged 10 ug
$600.00
RG200034 EMC9 (tGFP-tagged) - Human family with sequence similarity 158, member A (FAM158A) 10 ug
$489.00 $500.00
SC108275 EMC9 (untagged)-Human family with sequence similarity 158, member A (FAM158A) 10 ug
$300.00

Plasmid Map for RC200034

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200034
Circular map for RC200034

file-alt Citations

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