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TPPP3 (NM_016140) Human Tagged ORF Clone

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SKU
RC200020
TPPP3 (Myc-DDK-tagged)-Human tubulin polymerization-promoting protein family member 3 (TPPP3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TPPP3 or NM_016140"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TPPP3
Synonyms CGI-38; p20; p25gamma; TPPP/p20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200020 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCGAGCACAGACATGGCTGGGCTGGAGGAGAGCTTCCGCAAGTTTGCCATCCATGGTGACCCCA
AGGCCAGTGGGCAAGAGATGAATGGCAAGAACTGGGCCAAGCTGTGCAAGGACTGCAAGGTGGCTGACGG
AAAGTCCGTGACAGGGACCGATGTGGACATCGTCTTCTCCAAAGTCAAGGGGAAGTCTGCTCGGGTCATC
AACTATGAGGAGTTCAAGAAGGCCCTGGAAGAGCTGGCGACCAAGAGATTCAAGGGGAAGAGCAAGGAGG
AGGCCTTCGATGCCATCTGCCAGCTGGTGGCAGGCAAAGAGCCAGCCAATGTGGGCGTCACTAAAGCAAA
AACAGGGGGTGCTGTAGACCGGCTGACGGACACCAGCAGATACACGGGCTCCCACAAGGAGCGCTTCGAT
GAGAGCGGCAAGGGCAAGGGCATTGCGGGACGGCAGGACATCCTGGACGACAGTGGCTACGTGAGCGCCT
ACAAGAATGCAGGCACCTACGATGCCAAGGTGAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200020 protein sequence
Red=Cloning site Green=Tags(s)

MAASTDMAGLEESFRKFAIHGDPKASGQEMNGKNWAKLCKDCKVADGKSVTGTDVDIVFSKVKGKSARVI
NYEEFKKALEELATKRFKGKSKEEAFDAICQLVAGKEPANVGVTKAKTGGAVDRLTDTSRYTGSHKERFD
ESGKGKGIAGRQDILDDSGYVSAYKNAGTYDAKVKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016140
ORF Size 528 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016140.4
RefSeq Size 1159 bp
RefSeq ORF 531 bp
Locus ID 51673
UniProt ID Q9BW30, A0A024R702
Cytogenetics 16q22.1
MW 19 kDa
Summary Binds tubulin and has microtubule bundling activity. May play a role in cell proliferation and mitosis.UniProtKB/Swiss-Prot Function
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"NM_016140" in other vectors (4)
SKU Description Size Price
RC200020L3 Lenti ORF clone of Human tubulin polymerization-promoting protein family member 3 (TPPP3), Myc-DDK-tagged 10 ug
$600.00
RC200020L4 Lenti ORF clone of Human tubulin polymerization-promoting protein family member 3 (TPPP3), mGFP tagged 10 ug
$600.00
RG200020 TPPP3 (tGFP-tagged) - Human tubulin polymerization-promoting protein family member 3 (TPPP3) 10 ug
$489.00 $500.00
SC319358 TPPP3 (untagged)-Human tubulin polymerization-promoting protein family member 3 (TPPP3) 10 ug
$300.00

Plasmid Map for RC200020

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200020
Circular map for RC200020

file-alt Citations

Modulation Of Microtubule Acetylation By The Interplay Of TPPP/p25, SIRT2 And New Anticancer Agents With Anti-SIRT2 Potency
Szabó, Oláh, Szunyogh et al
Sci Rep (2017) 7 (1), 17070 DOI: 10.1038/s41598-017-17381-3
AI Summary: OriGene products, specifically the TPPP/p25 and α-synuclein constructs, were used for transfection experiments to study the competitive interactions affecting microtubule acetylation in HeLa cells.
Snippet: For the evaluation of the Venus signal, HeLa cells were transfected with the full length Venus (Addgene) or its fragments VN1–173 and VC155–238 (empty Venus) or VC-TPPP/p25 and VN-SIRT2 (0.3 μg of each plasmid), in the case of competition with an additional 0.1 µg TPPP/p2541 or α-synuclein construct (OriGene);
FigureFigureFigure
Showing 1-1 of 1 papers.
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